Kaïs Rtibi1,2, Slimen Selmi2, Dhekra Grami2, Khouloud Saidani2, Hichem Sebai2, Mohamed Amri1, Bruno Eto3, Lamjed Marzouki1,2. 1. Laboratoire de Neurophysiologie Fonctionnelle et Pathologies, Département des Sciences Biologiques, Faculté des Sciences de Tunis, 2092 Tunis, Tunisia. 2. Laboratoire de Nutrition et Physiologie Animale, Institut Supérieur de Biotechnologie de Béja, BP, 382-9000 Béja, Tunisia. 3. Laboratoires TransCell-Lab, Faculté de Médecine Xavier Bichât, 75018 Paris, France.
Abstract
BACKGROUND: This study was designed to investigate the effects of immature carob pod aqueous extract (ICPAE) on intestinal glucose absorption in vitro and in vivo using an oral glucose tolerance test (OGTT) as well as the potential antidiabetic effect in alloxan-induced diabetic rats. OGTT was carried by administration of glucose (2 g kg-1 , p.o.) and after treatment with extract (50, 100 and 200 mg kg-1 body weight). Diabetes was induced by single intraperitoneal injection of alloxan (150 mg kg-1 ). However, the extracts at various doses or glibenclamide (GLB, 10 mg kg-1 body weight) were given by oral administration for 2 weeks. RESULTS: ICPAE (50-2000 µg mL-1 ) exerted dose-dependent reduction of sodium-dependent glucose transport across isolated mice jejunum and the maximal inhibition exceeded 50%.The ICPAE treatment improved glucose tolerance. More importantly, ICPAE at various doses showed a significant reduction in blood glucose and biochemical profiles in diabetic rats. CONCLUSION: Our findings confirm that the degree of maturity of carob characterized by a different phytochemical composition may be responsible for these actions. Therefore, these compounds may be used as a food supplement in hyperglycemia and diabetes treatments.
BACKGROUND: This study was designed to investigate the effects of immature carob pod aqueous extract (ICPAE) on intestinal glucose absorption in vitro and in vivo using an oral glucose tolerance test (OGTT) as well as the potential antidiabetic effect in alloxan-induced diabeticrats. OGTT was carried by administration of glucose (2 g kg-1 , p.o.) and after treatment with extract (50, 100 and 200 mg kg-1 body weight). Diabetes was induced by single intraperitoneal injection of alloxan (150 mg kg-1 ). However, the extracts at various doses or glibenclamide (GLB, 10 mg kg-1 body weight) were given by oral administration for 2 weeks. RESULTS: ICPAE (50-2000 µg mL-1 ) exerted dose-dependent reduction of sodium-dependent glucose transport across isolated mice jejunum and the maximal inhibition exceeded 50%.The ICPAE treatment improved glucose tolerance. More importantly, ICPAE at various doses showed a significant reduction in blood glucose and biochemical profiles in diabeticrats. CONCLUSION: Our findings confirm that the degree of maturity of carob characterized by a different phytochemical composition may be responsible for these actions. Therefore, these compounds may be used as a food supplement in hyperglycemia and diabetes treatments.
Authors: María de la Fuente-Fernández; Daniel González-Hedström; Sara Amor; Antonio Tejera-Muñoz; Nuria Fernández; Luis Monge; Paula Almodóvar; Laura Andrés-Delgado; Luis Santamaría; Marin Prodanov; Antonio Manuel Inarejos-García; Angel Luis García-Villalón; Miriam Granado Journal: Antioxidants (Basel) Date: 2020-04-21