Alexandre Lamaro Cardoso1, Maria Márcia Bachion2, Júlia de Miranda Morais3, Marcelo Silva Fantinati4, Vera Lúcia Lima de Almeida5, Ruy Souza Lino6. 1. Fellow Master degree, Faculty of Medicine, Universidade Federal de Goiás (UFG), Goiania-GO, Brazil. Intellectual and scientific content of the study, conception and design of the study, manuscript writing. 2. PhD, Full Professor, Faculty of Nursing UFG, Goiania-GO, Brazil. Acquisition, analysis and interpretation of data. 3. PhD, Associate Professor, Faculty of Medicine, UFG, Goiania-GO, Brazil. Acquisition, analysis and interpretation of data. 4. PhD, Full Professor, ESEFFEGO, Universidade Estadual de Goiás (UEG), Goiania-GO, Brazil. Acquisition, analysis and interpretation of data. 5. Biomedic, Institute of Tropical Pathology and Public Health, Experimental Pathology Laboratory, UFG, Goiania-GO, Brazil. Acquisition of data. 6. PhD, Associate Professor, Institute of Tropical Pathology and Public Health, Experimental Pathology Laboratory, UFG, Goiania-GO, Brazil. Scientific and intellectual content of the study, analysis and interpretation of data, statistical analysis, manuscript preparation.
Abstract
PURPOSE: : To analyze the healing effects of stromal vascular fraction (SVF) application compared to wound dressing with 2% silver sulfadiazine in full thickness burn wounds in rats. METHODS: : Animals were divided into two groups: 2% silver sulfadiazine group and SVF group. Both groups received occlusive bandages while the first one was treated with 2% silver sulfadiazine and the latter was treated with injections of SVF prepared from adipose tissue extracted from an animal donor. The animals were accompanied through 3, 7 and 30 days for evaluation of macroscopic, microscopic and morphometric aspects. RESULTS: : On day three, a significant increase (p<0.05) of infiltration of polymorphonuclear, fibrin formation and fibroblasts migration in SVF group was observed. On the 7th day the mononuclear infiltrate, angiogenesis, collagen and fibroblasts were significantly increased in the SVF group (p<0.05). At 30 days significantly increased collagen deposition was observed in the SVF group (p<0.05) . CONCLUSION: : Adipose tissue derived stromal vascular fraction injections promotes better wound repair than 2% silver sulfadiazine in the treatment of full thickness burn in rats during the evaluated experimental period.
PURPOSE: : To analyze the healing effects of stromal vascular fraction (SVF) application compared to wound dressing with 2% silver sulfadiazine in full thickness burn wounds in rats. METHODS: : Animals were divided into two groups: 2% silver sulfadiazine group and SVF group. Both groups received occlusive bandages while the first one was treated with 2% silver sulfadiazine and the latter was treated with injections of SVF prepared from adipose tissue extracted from an animal donor. The animals were accompanied through 3, 7 and 30 days for evaluation of macroscopic, microscopic and morphometric aspects. RESULTS: : On day three, a significant increase (p<0.05) of infiltration of polymorphonuclear, fibrin formation and fibroblasts migration in SVF group was observed. On the 7th day the mononuclear infiltrate, angiogenesis, collagen and fibroblasts were significantly increased in the SVF group (p<0.05). At 30 days significantly increased collagen deposition was observed in the SVF group (p<0.05) . CONCLUSION: : Adipose tissue derived stromal vascular fraction injections promotes better wound repair than 2% silver sulfadiazine in the treatment of full thickness burn in rats during the evaluated experimental period.