Zhi-Qi Wu1, Hua-Guo Xu1. 1. Department of Laboratory Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu Province, China.
Abstract
OBJECTIVE: The purpose of our study was to analyze the effects of temperature, time delay, and time to centrifugation on the stability of human plasma adrenocorticotropin (ACTH) measurements. METHODS: Twenty-one EDTA whole blood sample pools were centrifuged at 1100 ×g for 10 minutes at 4°C either immediately or after storage for 2, 4, 8, and 24 hours at 4°C or room temperature. Plasma ACTH was then measured either immediately or after 2, 4, 8, and 24 hours storage at 4°C or room temperature. RESULTS: The change in ACTH concentrations was affected significantly (from 8.1±5.0% to 12.4±2.9% at 4 hours, P<.005) by time to centrifugation at room temperature. However, it remained stable (<5% change) up to 8 hours at 4°C in samples both centrifuged immediately and uncentrifuged. CONCLUSIONS: To get accurate values of plasma ACTH concentrations, if the samples cannot be transferred to the laboratory for analysis at room temperature within 2 hours, they should be immediately stored at 4°C, and analyzed within 8 hours.
OBJECTIVE: The purpose of our study was to analyze the effects of temperature, time delay, and time to centrifugation on the stability of human plasma adrenocorticotropin (ACTH) measurements. METHODS: Twenty-one EDTA whole blood sample pools were centrifuged at 1100 ×g for 10 minutes at 4°C either immediately or after storage for 2, 4, 8, and 24 hours at 4°C or room temperature. Plasma ACTH was then measured either immediately or after 2, 4, 8, and 24 hours storage at 4°C or room temperature. RESULTS: The change in ACTH concentrations was affected significantly (from 8.1±5.0% to 12.4±2.9% at 4 hours, P<.005) by time to centrifugation at room temperature. However, it remained stable (<5% change) up to 8 hours at 4°C in samples both centrifuged immediately and uncentrifuged. CONCLUSIONS: To get accurate values of plasma ACTH concentrations, if the samples cannot be transferred to the laboratory for analysis at room temperature within 2 hours, they should be immediately stored at 4°C, and analyzed within 8 hours.
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