Isolation and Simultaneous Quantification of Nine Triterpenoids from Rosa davurica Pall.

Rosa davurica Pall. has been used as a traditional Oroqen medicine to treat dyspepsia, gastroenterologia, menoxenia and other diseases. In this study, the chemical constituents research on the fruit pulp of R. davurica led to the isolation and identification of nine bioactive pentacyclic triterpenoids and five of them were isolated from the plant for the first time. Subsequently a simple and rapid high-performance liquid chromatography (HPLC) method was established for the simultaneous quantification of the nine triterpenoids. The separation was performed on a Merges reverse phase (RP) C18 (250 × 4.6 mm, 5 μm) column through 40 min gradient delivery of 0.05% phosphoric acid aqueous solution and acetonitrile at a flow rate of 1.2 mL/min at 30°C and the detection wavelength selected was 210 nm. All of the calibration curves showed good linearity (R2 > 0.9990) in the tested ranges. The limit of detection and the limit of quantitation were in the range of 0.21-1.27 μg/mL and 0.63-3.80 μg/mL, respectively. The established method also showed good precision, repeatability and recovery and can be used to the routine quality control of R. davurica and other herbs containing pentacyclic triterpenoids.