Eunice Molinar-Toribio1,2, Elisabet Fuguet3,4, Sara Ramos-Romero1, Núria Taltavull5, Lucía Méndez6, M Rosa Nogués5, Isabel Medina6, Josep Lluís Torres1, Jara Pérez-Jiménez7,8. 1. Institute of Advanced Chemistry of Catalonia (IQAC-CSIC), Barcelona, Spain. 2. Department of Biochemistry, Faculty of Natural, Exact Sciences and Technology, University of Panama, Panama City, Panama. 3. Departament de Química Analítica, Universitat de Barcelona, Barcelona, Spain. 4. Serra-Húnter Programme, Generalitat de Catalunya, Barcelona, Spain. 5. Unidad de Farmacología, Facultad de Medicina y Ciencias de la Salud, Universidad Rovira i Virgili, Reus, Spain. 6. Instituto de Investigaciones Marinas (IIM-CSIC), Vigo, Spain. 7. Institute of Advanced Chemistry of Catalonia (IQAC-CSIC), Barcelona, Spain. jara.perez@ictan.csic.es. 8. Department of Metabolism and Nutrition, Institute of Food Science, Technology and Nutrition (ICTAN-CSIC), José Antonio Novais 10, 28040, Madrid, Spain. jara.perez@ictan.csic.es.
Abstract
PURPOSE: Polyphenol metabolites are key mediators of the biological activities of polyphenols. This study aimed to evaluate the long-term effects of a high-fat high-sucrose (HFHS) diet on the metabolism of proanthocyanidins from grape seed extract (GSE). METHODS: Adult female Wistar-Kyoto rats were fed a standard (STD) or HFHS diet supplemented or not with GSE for 16 weeks. PA metabolites were determined by targeted HPLC-MS/MS analysis. RESULTS: A lower concentration of total microbial-derived PA metabolites was present in urine and the aqueous fraction of faeces in the HFHS + GSE group than in the STD + GSE group. In contrast, a tendency towards the formation of conjugated (epi)catechin metabolites in the HFHS + GSE group was observed. CONCLUSIONS: These results show that a HFHS diet significantly modifies PA metabolism, probably via: (1) a shift in microbial communities not counteracted by the polyphenols themselves; and (2) an up-regulation of hepatic enzymes.
PURPOSE:Polyphenol metabolites are key mediators of the biological activities of polyphenols. This study aimed to evaluate the long-term effects of a high-fat high-sucrose (HFHS) diet on the metabolism of proanthocyanidins from grape seed extract (GSE). METHODS: Adult female Wistar-Kyoto rats were fed a standard (STD) or HFHS diet supplemented or not with GSE for 16 weeks. PA metabolites were determined by targeted HPLC-MS/MS analysis. RESULTS: A lower concentration of total microbial-derived PA metabolites was present in urine and the aqueous fraction of faeces in the HFHS + GSE group than in the STD + GSE group. In contrast, a tendency towards the formation of conjugated (epi)catechin metabolites in the HFHS + GSE group was observed. CONCLUSIONS: These results show that a HFHS diet significantly modifies PA metabolism, probably via: (1) a shift in microbial communities not counteracted by the polyphenols themselves; and (2) an up-regulation of hepatic enzymes.
Authors: Peter J Turnbaugh; Ruth E Ley; Michael A Mahowald; Vincent Magrini; Elaine R Mardis; Jeffrey I Gordon Journal: Nature Date: 2006-12-21 Impact factor: 49.962
Authors: Eunice Molinar-Toribio; Jara Pérez-Jiménez; Sara Ramos-Romero; Livia Gómez; Núria Taltavull; Maria Rosa Nogués; Alberto Adeva; Olga Jáuregui; Jesús Joglar; Pere Clapés; Josep Lluís Torres Journal: Food Funct Date: 2015-07-01 Impact factor: 5.396
Authors: David S Wishart; Dan Tzur; Craig Knox; Roman Eisner; An Chi Guo; Nelson Young; Dean Cheng; Kevin Jewell; David Arndt; Summit Sawhney; Chris Fung; Lisa Nikolai; Mike Lewis; Marie-Aude Coutouly; Ian Forsythe; Peter Tang; Savita Shrivastava; Kevin Jeroncic; Paul Stothard; Godwin Amegbey; David Block; David D Hau; James Wagner; Jessica Miniaci; Melisa Clements; Mulu Gebremedhin; Natalie Guo; Ying Zhang; Gavin E Duggan; Glen D Macinnis; Alim M Weljie; Reza Dowlatabadi; Fiona Bamforth; Derrick Clive; Russ Greiner; Liang Li; Tom Marrie; Brian D Sykes; Hans J Vogel; Lori Querengesser Journal: Nucleic Acids Res Date: 2007-01 Impact factor: 16.971
Authors: Esther García-Díez; Marta Cuesta-Hervás; Ana M Veses-Alcobendas; Óscar Alonso-Gordo; Elena García-Maldonado; Miriam Martínez-Suárez; Beatriz Herranz; M Pilar Vaquero; María Dolores Álvarez; Jara Pérez-Jiménez Journal: Eur J Nutr Date: 2021-01-02 Impact factor: 5.614