Simone Stauffacher1, Adrian Lussi1, Sandor Nietzsche2, Klaus W Neuhaus3, Sigrun Eick4. 1. Department of Preventive, Restorative and Pediatric Dentistry, University of Bern, Freiburgstrasse 7, CH-3010, Bern, Switzerland. 2. Center of Electron Microscopy, University Hospital of Jena, Jena, Germany. 3. Department of Preventive, Restorative and Pediatric Dentistry, University of Bern, Freiburgstrasse 7, CH-3010, Bern, Switzerland. klaus.neuhaus@zmk.unibe.ch. 4. Laboratory of Oral Microbiology, Department of Periodontology, University of Bern, Bern, Switzerland.
Abstract
OBJECTIVES: We wanted to investigate differences in invasiveness into radicular dentinal tubules by monocultured and co-cultured bacteria frequently found in infected root canals. METHODS: Fifty-one human roots were incubated for 8 weeks with monocultured Streptococcus gordonii ATCC 10558, Streptococcus sanguinis ATCC 10556, and with five capnophiles/anaerobes as well as with capnophiles/anaerobes co-cultured with a streptococcal species. Thereafter, bacterial samples were cultured from the inner, middle, and outer third of the root dentine of longitudinally broken teeth (n = 5). In addition, scanning electron microscopy (SEM) images were obtained. RESULTS: Single gram-positive species were able to penetrate into the middle and outer third of the root dentine. Fusobacterium nucleatum ATCC 25586 was not found in any of the dentine specimens. Prevotella intermedia ATCC 25611 and Porphyromonas gingivalis ATCC 33277 were found in the inner and middle third. The bacterial load of streptococci was higher in all thirds in co-cultures compared to single infections. In co-cultures with streptococci, Actinomyces oris ATCC 43146 was found in the outer third in 9/10 samples, whereas P. intermedia ATCC 25611 was not detectable inside dentine. Co-culture with S. sanguinis ATCC 10556 enabled F. nucleatum ATCC 25586 to invade dentine; SEM images showed that F. nucleatum ATCC 25586 had a swollen shape. CONCLUSIONS: Invasiveness of bacteria into dentinal tubules is species-specific and may change depending on culturing as a single species or co-culturing with other bacteria. CLINICAL RELEVANCE: Oral streptococci may promote or inhibit invasion of capnophiles/anaerobes into radicular dentine.
OBJECTIVES: We wanted to investigate differences in invasiveness into radicular dentinal tubules by monocultured and co-cultured bacteria frequently found in infected root canals. METHODS: Fifty-one human roots were incubated for 8 weeks with monocultured Streptococcus gordoniiATCC 10558, Streptococcus sanguinis ATCC 10556, and with five capnophiles/anaerobes as well as with capnophiles/anaerobes co-cultured with a streptococcal species. Thereafter, bacterial samples were cultured from the inner, middle, and outer third of the root dentine of longitudinally broken teeth (n = 5). In addition, scanning electron microscopy (SEM) images were obtained. RESULTS: Single gram-positive species were able to penetrate into the middle and outer third of the root dentine. Fusobacterium nucleatumATCC 25586 was not found in any of the dentine specimens. Prevotella intermedia ATCC 25611 and Porphyromonas gingivalis ATCC 33277 were found in the inner and middle third. The bacterial load of streptococci was higher in all thirds in co-cultures compared to single infections. In co-cultures with streptococci, Actinomyces oris ATCC 43146 was found in the outer third in 9/10 samples, whereas P. intermedia ATCC 25611 was not detectable inside dentine. Co-culture with S. sanguinis ATCC 10556 enabled F. nucleatumATCC 25586 to invade dentine; SEM images showed that F. nucleatumATCC 25586 had a swollen shape. CONCLUSIONS: Invasiveness of bacteria into dentinal tubules is species-specific and may change depending on culturing as a single species or co-culturing with other bacteria. CLINICAL RELEVANCE: Oral streptococci may promote or inhibit invasion of capnophiles/anaerobes into radicular dentine.
Authors: B P F A Gomes; E T Pinheiro; C R Gadê-Neto; E L R Sousa; C C R Ferraz; A A Zaia; F B Teixeira; F J Souza-Filho Journal: Oral Microbiol Immunol Date: 2004-04
Authors: Adriana L Santos; José F Siqueira; Isabela N Rôças; Ederson C Jesus; Alexandre S Rosado; James M Tiedje Journal: PLoS One Date: 2011-11-21 Impact factor: 3.240
Authors: Janir Alves Soares; Maria Auxiliadora Roque de Carvalho; Suelleng Maria Cunha Santos Soares; Rodrigo Dantas Pereira; Manoel Brito-Júnior; Paula Prazeres Magalhães; Isabel Alessandra Miranda Nunes; Rudys Rodolpho de Jesus Tavarez; Claudia de Castro Rizzi; Rafaela Nogueira Moreira; Luiz de Macêdo Farias; Etevaldo Matos Maia Filho Journal: ScientificWorldJournal Date: 2019-01-01