A C S Marinho1, F C Martinho1, L M Gonçalves2, H R C Rabang1, B P F A Gomes1. 1. Department of Restorative Dentistry, Endodontics Division, Piracicaba Dental School - State University of Campinas- UNICAMP, Piracicaba, SP, Brazil. 2. Postgraduate Program in Dentistry, Ceuma University-UniCEUMA, São Luis, MA, Brazil.
Abstract
AIM: To evaluate the effectiveness of Reciproc for the removal of cultivable bacteria and endotoxins from root canals in comparison with multifile rotary systems. METHODOLOGY: The root canals of forty human single-rooted mandibular pre-molars were contaminated with an Escherichia coli suspension for 21 days and randomly assigned to four groups according to the instrumentation system: GI - Reciproc (VDW); GII - Mtwo (VDW); GIII - ProTaper Universal (Dentsply Maillefer); and GIV -FKG Race(™) (FKG Dentaire) (n = 10 per group). Bacterial and endotoxin samples were taken with a sterile/apyrogenic paper point before (s1) and after instrumentation (s2). Culture techniques determined the colony-forming units (CFU) and the Limulus Amebocyte Lysate assay was used for endotoxin quantification. Results were submitted to paired t-test and anova. RESULTS: At s1, bacteria and endotoxins were recovered in 100% of the root canals investigated (40/40). After instrumentation, all systems were associated with a highly significant reduction of the bacterial load and endotoxin levels, respectively: GI - Reciproc (99.34% and 91.69%); GII - Mtwo (99.86% and 83.11%); GIII - ProTaper (99.93% and 78.56%) and GIV - FKG Race(™) (99.99% and 82.52%) (P < 0.001). No statistical difference were found amongst the instrumentation systems regarding bacteria and endotoxin removal (P > 0.01). CONCLUSION: The reciprocating single file, Reciproc, was as effective as the multifile rotary systems for the removal of bacteria and endotoxins from root canals.
AIM: To evaluate the effectiveness of Reciproc for the removal of cultivable bacteria and endotoxins from root canals in comparison with multifile rotary systems. METHODOLOGY: The root canals of forty human single-rooted mandibular pre-molars were contaminated with an Escherichia coli suspension for 21 days and randomly assigned to four groups according to the instrumentation system: GI - Reciproc (VDW); GII - Mtwo (VDW); GIII - ProTaper Universal (Dentsply Maillefer); and GIV -FKG Race(™) (FKG Dentaire) (n = 10 per group). Bacterial and endotoxin samples were taken with a sterile/apyrogenic paper point before (s1) and after instrumentation (s2). Culture techniques determined the colony-forming units (CFU) and the Limulus Amebocyte Lysate assay was used for endotoxin quantification. Results were submitted to paired t-test and anova. RESULTS: At s1, bacteria and endotoxins were recovered in 100% of the root canals investigated (40/40). After instrumentation, all systems were associated with a highly significant reduction of the bacterial load and endotoxin levels, respectively: GI - Reciproc (99.34% and 91.69%); GII - Mtwo (99.86% and 83.11%); GIII - ProTaper (99.93% and 78.56%) and GIV - FKG Race(™) (99.99% and 82.52%) (P < 0.001). No statistical difference were found amongst the instrumentation systems regarding bacteria and endotoxin removal (P > 0.01). CONCLUSION: The reciprocating single file, Reciproc, was as effective as the multifile rotary systems for the removal of bacteria and endotoxins from root canals.
Authors: Frederico C Martinho; Bruna J M Corazza; Rayana D Khoury; Esteban I F Orozco; Cassia C Toia; Felipe P Machado; Marcia C Valera Journal: Clin Oral Investig Date: 2022-06-20 Impact factor: 3.573
Authors: Gustavo G Nascimento; Diego G D Rabello; Bruna J M Corazza; Ana P M Gomes; Eduardo G Silva; Frederico C Martinho Journal: Sci Rep Date: 2021-01-13 Impact factor: 4.379
Authors: Ezequiel S Gabrielli; Augusto R Lima; Priscila A Francisco; Daniel R Herrera; Adriana de-Jesus-Soares; Caio C R Ferraz; José Flávio A Almeida; Marina A Marciano; Brenda P F A Gomes Journal: Clin Oral Investig Date: 2021-06-11 Impact factor: 3.573