Puralae Channabasavaiah Jagadish1, Kotehal Parameshwarappa Latha2, Jayesh Mudgal3, Gopalan Kutty Nampurath4. 1. Department of Pharmaceutical Quality Assurance, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal 576104, Karnataka, India. 2. Department of Chemistry, P.G. Studies and Research in Chemistry, Sahyadri Science College (Autonomous), Shivamogga 577201, Karnataka, India. 3. Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal 576104, Karnataka, India. 4. Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal 576104, Karnataka, India. Electronic address: ng.kutty@manipal.edu.
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE: The rhizomes of an acaulescent perennial herb, Kaempferia galanga Linn (Family: Zingiberaceae), used as traditional ayurvedic herb to get relief from indigestion, swelling, pain, high blood pressure and dyslipidemia. AIM OF THE STUDY: To prepare and characterize various extracts of Kaempferia galanga (K. galanga) for their comparative evaluation for the identification of the most efficacious extract and its possible pharmacological implication in acute and chronic inflammatory paradigm. MATERIALS AND METHODS: Dried and powdered rhizome of K. galanga was subjected to alcoholic extraction as well as successive extractions with various solvents. After phytochemical characterization, all the extracts were standardized for the presence of ethyl-p-methoxycinnamate. The extracts, and the isolated compound, were tested against carrageenan-induced acute inflammation in rats. The most promising extract was tested against adjuvant-induced chronic inflammation in rats. Further, local myeloperoxidase (MPO) levels were investigated to establish the possible mechanism of action. RESULTS: Among the extracts, petroleum ether extract (SKG-1) and crude alcoholic extract (KG) had the maximum quantity of ethyl-p-methoxycinnamate. SKG-1 (300mg/kg) was found effective against acute inflammation in rats. Further, SKG-1 (100mg/kg) reversed the inflammation and elevated MPO levels found in the chronic model. CONCLUSIONS: The results suggest that among all the extracts of K. galanga, SKG-1 effectively suppresses the progression of acute and chronic inflammation in rats by inhibition of neutrophil infiltration.
ETHNOPHARMACOLOGICAL RELEVANCE: The rhizomes of an acaulescent perennial herb, Kaempferia galanga Linn (Family: Zingiberaceae), used as traditional ayurvedic herb to get relief from indigestion, swelling, pain, high blood pressure and dyslipidemia. AIM OF THE STUDY: To prepare and characterize various extracts of Kaempferia galanga (K. galanga) for their comparative evaluation for the identification of the most efficacious extract and its possible pharmacological implication in acute and chronic inflammatory paradigm. MATERIALS AND METHODS: Dried and powdered rhizome of K. galanga was subjected to alcoholic extraction as well as successive extractions with various solvents. After phytochemical characterization, all the extracts were standardized for the presence of ethyl-p-methoxycinnamate. The extracts, and the isolated compound, were tested against carrageenan-induced acute inflammation in rats. The most promising extract was tested against adjuvant-induced chronic inflammation in rats. Further, local myeloperoxidase (MPO) levels were investigated to establish the possible mechanism of action. RESULTS: Among the extracts, petroleum ether extract (SKG-1) and crude alcoholic extract (KG) had the maximum quantity of ethyl-p-methoxycinnamate. SKG-1 (300mg/kg) was found effective against acute inflammation in rats. Further, SKG-1 (100mg/kg) reversed the inflammation and elevated MPO levels found in the chronic model. CONCLUSIONS: The results suggest that among all the extracts of K. galanga, SKG-1 effectively suppresses the progression of acute and chronic inflammation in rats by inhibition of neutrophil infiltration.
Authors: Abdelsamed I Elshamy; Tarik A Mohamed; Ahmed F Essa; Ahmed M Abd-ElGawad; Ali S Alqahtani; Abdelaaty A Shahat; Tatsuro Yoneyama; Abdel Razik H Farrag; Masaaki Noji; Hesham R El-Seedi; Akemi Umeyama; Paul W Paré; Mohamed-Elamir F Hegazy Journal: Nutrients Date: 2019-10-07 Impact factor: 5.717
Authors: Angela Ladurner; Martin Zehl; Ulrike Grienke; Christoph Hofstadler; Nadina Faur; Fátima C Pereira; David Berry; Verena M Dirsch; Judith M Rollinger Journal: Front Pharmacol Date: 2017-07-17 Impact factor: 5.810