| Literature DB >> 27718551 |
Yihao Zhang1,2, Long Qian3, Weijia Wei1,4,5, Yu Wang1, Beining Wang1, Pingping Lin1, Wenchao Liu1, Luze Xu1, Xiang Li1, Dongming Liu1, Sida Cheng1, Jiaofeng Li1, Yixuan Ye1, Hang Li1, Xiaohan Zhang1, Yiming Dong2, Xuejin Zhao4, Cuihua Liu6, Haoqian M Zhang2, Qi Ouyang2,7, Chunbo Lou4,5.
Abstract
We developed an in vitro DNA detection system using a pair of dCas9 proteins linked to split halves of luciferase. Luminescence was induced upon colocalization of the reporter pair to a ∼44 bp target sequence defined by sgRNAs. We used the system to detect Mycobacterium tuberculosis DNA with high specificity and sensitivity. The reprogrammability of dCas9 was further leveraged in an array design that accesses sequence information across the entire genome.Entities:
Keywords: DNA detection; dCas9; in vitro pathogenic detection; paired design; split luciferase; tuberculosis
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Year: 2016 PMID： 27718551 DOI： 10.1021/acssynbio.6b00215
Source DB: PubMed Journal: ACS Synth Biol ISSN： 2161-5063 Impact factor: 5.110