The safety of nanostructured synthetic amorphous silica (SAS) as a food additive (E 551).

KEY MESSAGES: Particle sizes of E 551 products are in the micrometre range. The typical external diameters of the constituent particles (aggregates) are greater than 100 nm. E 551 does not break down under acidic conditions such as in the stomach, but may release dissolved silica in environments with higher pH such as the intestinal tract. E 551 is one of the toxicologically most intensively studied substances and has not shown any relevant systemic or local toxicity after oral exposure. Synthetic amorphous silica (SAS) meeting the specifications for use as a food additive (E 551) is and has always been produced by the same two production methods: the thermal and the wet processes, resulting in E 551 products consisting of particles typically in the micrometre size range. The constituent particles (aggregates) are typically larger than 100 nm and do not contain discernible primary particles. Particle sizes above 100 nm are necessary for E 551 to fulfil its technical function as spacer between food particles, thus avoiding the caking of food particles. Based on an in-depth review of the available toxicological information and intake data, it is concluded that the SAS products specified for use as food additive E 551 do not cause adverse effects in oral repeated-dose studies including doses that exceed current OECD guideline recommendations. In particular, there is no evidence for liver toxicity after oral intake. No adverse effects have been found in oral fertility and developmental toxicity studies, nor are there any indications from in vivo studies for an immunotoxic or neurotoxic effect. SAS is neither mutagenic nor genotoxic in vivo. In intact cells, a direct interaction of unlabelled and unmodified SAS with DNA was never found. Differences in the magnitude of biological responses between pyrogenic and precipitated silica described in some in vitro studies with murine macrophages at exaggerated exposure levels seem to be related to interactions with cell culture proteins and cell membranes. The in vivo studies do not indicate that there is a toxicologically relevant difference between SAS products after oral exposure. It is noted that any silicon dioxide product not meeting established specifications, and/or produced to provide new functionality in food, requires its own specific safety and risk assessment.

Introduction

Synthetic amorphous silica (SAS), also known as synthetic amorphous silicon dioxide, has been used as a direct food additive for decades. Both the Joint FAO/WHO Expert Committee on Food Additives (JECFA) and the EU Scientific Committee on Food (SCF, now EFSA) have previously evaluated silicon dioxide as a food additive (E 551, INS 551) and established an acceptable daily intake (ADI) “not specified” which generally refers to substances of very low toxicity. The more recent designation of SAS as a nanostructured material has, however, raised concerns with regard to its safety as a food additive and has prompted several new investigations as well as safety and risk assessments by various research groups. This article has therefore been written to specifically address these new safety concerns, including the possibility of low-dose effects, toxicity of potentially released nanoparticles, liver toxicity, and immunotoxicity.

Background: SAS as a direct food additive (E 551, INS 551)

Specific purity criteria are defined for the use of SAS as a food additive. In the European Union (EU), the specific purity criteria are defined in Commission Regulation (EU) No 231/2012. SAS meeting these criteria is permitted under the name of “Silicon dioxide” or “E 551” as direct food additive in accordance with Annex II and Annex III to Regulation (EC) No. 1333/2008 (as amended) as an anti-caking agent and a carrier. The specific criteria in Commission Regulation (EU) No. 231/2012 also contain the two production methods with which SAS is made: the thermal process and the wet process. These processes result in solid SAS products of identical chemical composition either as anhydrous products (pyrogenic SAS, produced by the thermal route) or as hydrated products (precipitated silica, silica gel, or hydrous silica; all produced by the wet route). An overview of SAS products which are used as a food additive (E 551) is presented in Table 1.

Table 1

Overview on synthetic amorphous silica products used as a food additive (E 551)

Product	EU name (Reg. 231/2012)	EINECS no	CAS no., generic	CAS no., specific	Chemical abstracts index name	JRCa name
Pyrogenic silica	Fumed silica	231-545-4	7631-86-9	112945-52-5	Silica, amorphous, fumed; crystalline-free	NM-202 NM-203
Hydrated silica	Precipitated silica	231-545-4	7631-86-9	112926-00-8	Synthetic amorphous silica, precipitated; crystalline-free	NM-200 NM-201 NM-204
	Silica gel, hydrous silica	231-545-4	7631-86-9	112926-00-8	Synthetic amorphous silica, gel; crystalline-free

a JRC EU Joint Research Centre

SAS as used as a food additive (E 551, INS 551) is marketed as a white fluffy powder or as granules which, in the case of hydrated silica, may contain surface- or pore-bound water. Importantly, E 551 is not marketed as a suspension of stabilised nanoparticles (colloidal silica). Since the beginning of its commercialisation in the 1950s (ECETOC 2006), SAS is produced by the same two manufacturing processes, i.e. the thermal process and the wet process. These two production methods have been described earlier (EC 2007). The resulting products are chemically identical; products made by the wet process may contain sodium salts as impurities resulting from the manufacturing process based on sodium silicates. E 551 is placed on the market in solid form only and should therefore not be confused with stabilised suspensions of silica nanoparticles (often referred to as colloidal silica). The latter are manufactured by different processes, e.g. the Stöber method, and do not meet the EU specifications for E 551. These colloidal silica suspensions are not regulated as direct food additive (E 551) in the EU, but may be used as processing aid in EU countries under national legislation and be marketed as food grade.

E 551 is not produced in a nano- and non-nanoform, nor does it exist in a nano- and non-nanoform. E 551 is not engineered to have novel properties, and the particle size distributions of aggregates and agglomerates of today’s products are in fact identical to those produced in earlier decade. Since the technical function of E 551 is to act as a spacer between food components in order for them to remain in a free state, nanosized particles are actually not desired because they are too small to enable this effect. The spacer function can only be achieved by the silica aggregates and agglomerates having size ranges which are typically greater than 100 nm (it is noted that there are studies published, where almost spherical corn starch host particles with a smooth surface were used and where adsorbate diameters of anti-caking agents down to 40–50 nm are mentioned (Kurfeβ et al. 2005; Müller et al. 2008; Ruppel et al. 2009). Such powders do, however, not represent the typical host powder where larger spacers are required to fill cavities). Regulatory aspects relating to the EU food sector have recently been summarised and are therefore not re-iterated here (Amenta et al. 2015).

E 551 particle morphology and size

E 551 is produced as fluffy fine powder or granules in the micrometre size range. Figure 1 (left) shows a ca. 20–30 micrometre granule as typically contained in products delivered to the customer. Powders and granules consist of agglomerated aggregates (see Fig. 1, middle). Agglomerates can be separated into aggregates (see Fig. 1, right) by applying high energy or shear force techniques such as prolonged ultrasonication or mixing.

Fig. 1

SEM images of pyrogenic silica granule, agglomerate, and aggregate (©Evonik Resource Efficiency GmbH)

These aggregates are the smallest discrete entities in E 551; they are three-dimensional units with a high degree of branching. The typical external size of SAS aggregates is greater than 100 nm; separation of aggregates into “primary particles” is impossible (Dünisch 2005; ECETOC 2006; Gray and Muranko 2006; Ma-Hock et al. 2007; Maier et al. 2006). The aggregates can therefore be designated as the constituent particles of E 551. Primary particles—according to the ISO definition “the original source particles of agglomerates or aggregates or mixtures of the two”—are not discernible anymore in E 551 after completion of the synthesis process. All primary particles have been fused together to form the three-dimensional aggregates. No inner boundaries are visible within SAS aggregates (Albers et al. 2015), see Fig. 2.

Fig. 2

TEM images of part of a pyrogenic silica aggregate (left) and enlarged view of inner structures at the nanometre scale (middle, right) (©Evonik Resource Efficiency GmbH)

Because there are no discernible “primary particles” anymore in the E 551 product, it is impossible to determine their size or size distribution. With regard to aggregate sizes and aggregate size distributions, several techniques are usually necessary for a reliable volume- or mass-based size determination. Generally, external aggregate sizes are greater than 100 nm (data on file), but sample preparation methods and the analytical method have a profound influence on the results (Barahona et al. 2016). If not used in combination with other techniques, 2-dimensional ultrascopy methods (scanning electron microscopy, SEM, or transmission electron microscopy, TEM), in particular, are not suitable to determine SAS aggregate size distributions. In the case of branched aggregates, such as SAS, 2-dimensional SEM and TEM generate so-called equivalent diameters and an apparent (i.e. not a real) property of SAS, namely that it would contain small isolated particles in the nanosize range. The European Commission JRC report on the requirements on measurements for the implementation of the European Commission definition of the term “nanomaterial” reads (page 22 of the report) (Linsinger et al. 2012): “…It has been suggested that electron microscopy (EM) [in particular, transmission electron microscopy (TEM)] provides the most accurate particle size values. This, however, relies on the assumption that a projected area equivalent size is the ‘true size’. Unfortunately, this size is deduced from a 2D image and is independent of the thickness of the particle in the third dimension. A second disadvantage is that it does not correspond to common understanding: the area equivalent size of a highly branched particle may be very small, whereas it can extend widely, reaching a large external dimension”. It is furthermore known that the preparation of representative samples is a major source of uncertainty, and that the automated counting of digitally processed TEM and SEM images generates artificially high numbers of particles in the nanosize range when agglomerates are present. Reports based solely on TEM/SEM methodology and claiming that 100 % of E 551 would be in the nanosize range are therefore misleading (Agir pour l’environnement 2016). Currently, there are no reliable standardised analytical methods available to characterise the number weighted particle size distribution of SAS in the nanosize range below 100 nm. Efforts to establish such distributions suffer from the assumption of a spherical particle shape, see, for example, (Barahona et al. 2016; Contado et al. 2016), which is not correct in the case of E 551 as the E 551 aggregate is not a sphere.

Exposure and intake estimates

Information on relevant food categories and use levels of E 551 were recently collected by members of the Association of Synthetic Amorphous Silica Producers (ASASP) from customers and from food associations. This was the only way for the raw material suppliers to gain this information. According to the survey, the uses covered were all direct uses and carry-over into foods. The collected data were used to generate intake estimates with the Comprehensive European Dietary Exposure Model (CEDEM) (Tennant 2016) and resulted in population average intake estimates of silicon dioxide from its use as a food additive (E 551) ranging between 0.28 and 4.53 mg E 551/kg bw/day. The highest intake estimate was at 12.7 mg/kg bw/day for children in Bulgaria which is likely to be an overestimate because it assumes 100 % occurrence of E 551 in all food categories. Earlier, a daily exposure of 9.4 mg/kg bw/day was estimated for the Dutch population based on expert judgement of consumption frequencies and amounts (Dekkers et al. 2011).

For the total dietary intake of E 551, not only the amount of E 551 in food has to be estimated, but also potential intake from other sources. In addition to its use as a food additive, E 551 is also used in cosmetics (e.g. as abrasion additive in toothpastes), in pharmaceuticals (e.g. as free-flow additive, carrier, or retardant agent and as tableting aid), and in dietary supplements (e.g. as dispersive medium for vitamins). Some naturally occurring foods, particularly of plant origin, contain high amounts of biogenic silicon dioxide, amongst them cereals, particularly oats and barley, wheat flour, rice, and, especially, beer (see, for example, EFSA 2009; Jugdaohsingh 2007; Powell et al. 2005). The difficulty in analysing food matrices for E 551 particles and to differentiate these from the high natural silicon and silicon dioxide (silica) content of many foods make a direct measurement difficult. Usually dissolved silicon is extracted and measured, but there are currently no validated routine methods for the quantitative determination of silicon dioxide particles in food (Singh et al. 2014). Current laboratory methods for silica particle analysis are only validated for the analysis in relatively simple and defined matrices and often have a low sensitivity in the size range below 200 nm and a low reproducibility.

In food supplements, silica is added up to a level of 700 mg silicon/day (EFSA 2009). Estimates on the intake of E 551 contained in pharmaceutical formulations are not available. Very low levels (<0.2 %) are required when used as a glidant for tablets, the most common medicinal application. Toothpaste may, however, contain relatively high amounts of E 551, which in part may be swallowed.

An overview of intakes from different sources is provided in Table 2.

Table 2

Silicon and Silicon dioxide (silica particle) intakes from different sources

	Source	Daily intake		References
Silicon (Si)		mg/day	mg/kg bw/day
	Food (naturally and from additives), Western population	20–50	0.3–0.8	EFSA (2004), Jugdaohsingh (2007) and Jurkić et al. (2013)
	Food (mainly plant based)	140–204		Cited in Jugdaohsingh (2007)
	Food (BE)	18.6 ± 8.5		Robberecht et al. (2009)
	Beer (1 litre)	6.4–56.5		Casey and Bamforth (2010)
	Dietary supplements	1–75, up to 700	0.017–1.5, up to 12	EFSA (2004, 2009)
Silica (SiO2)
	E 551 in food (NL)	658124 (“nanosilica”)	9.4 (“dissolved”)1.8 (“nanosilica”a)	Dekkers et al. (2011)
	E 551 in food		0.28–4.53	(FCRA 2016)
	Dietary supplements	up to 1500	up to 25	EFSA (2009)
	Mixed silicate particles in food	35 (0–254)		Lomer et al. (2004)
	Toothpaste containing 30 % SAS	41	0.65	Using intake data from (SCCS 2015)b
	E 551 in medicines	0.2		Based on two oral tablets/day à 0.5 g with 0.02 % E 551

aParticle size range 1–200 nm

b(SCCS 2015): daily intake, toothpaste = 138 mg or 2.16 mg/kg bw

Bioavailability after oral intake

Due to the changing and complex conditions in the gastrointestinal tract, including different pH environments, the influence of food matrices, microflora, mucus, and peristaltic movements, it is difficult to fully predict the fate of E 551 particles after oral ingestion. Due to analytical difficulties mainly because of high environmental background silica levels, no studies are available in which unlabelled silica particles were quantitatively analysed in body fluids and organs after oral exposure. Results of in vitro digestion studies (Maier et al. 2013; Peters et al. 2012; Sakai-Kato et al. 2014) have shown a resistance of silica agglomerates and aggregates to break down under conditions of low pH such as in the stomach. Dissolution may increase in the more alkaline pH environments of the small intestine and in the colon, and values of up to 20 wt% have been reported for the dissolution in the intestinal content (van der Zande et al. 2014). These findings are in agreement with biodurability tests performed with SAS in Caco-2 medium by the European Joint Research Centre (JRC 2013). A fraction of E 551 may therefore be taken up in form of orthosilicic acid by the intestinal tract. If absorbed, particles are usually sequestered in Peyer’s patch macrophages, and those that escape sequestration are transported by lymph rather than by portal blood. It has been suggested that the nanosized fraction of ingested particles could directly pass through regular epithelial cells to underlying dendritic cells (Howe et al. 2014), but there are no studies demonstrating this for SAS. Given the high exposure to natural silica in foods, and the lack of any signs of an immunotoxic potential of SAS in repeated-dose animal studies, an adverse effect by this mechanism is unlikely.

In an oral 28-d study in rats with food-grade precipitated silica, mesenteric lymph nodes, liver, and kidney were investigated by electron microscopy for silica particles. Occasionally, cells of the mesenteric lymph nodes, liver, and kidneys of all animals of the untreated group and of the amorphous silica-treated group showed electron dense structures. These electron dense structures were found in vacuoles in the cytoplasm and were characterised as “irregular homogenous to fine granular material”. The granular structures measured only few nanometres, but did not show the shape or appearance of amorphous silica. These results show the presence of particulate matter in the nanosize range in ALL animals, i.e. including those NOT exposed to precipitated silica (CEFIC 2011). Silica particles could also not be found in the livers of rats exposed for 28 or 84 days to food-grade pyrogenic silica (van der Zande et al. 2014). Silicon (Si) concentrations were not increased in the investigated organs (liver, kidney, testis, brain) even after high-dose exposure for up to 84 days. An increase in Si levels was only found in the spleen of rats exposed to an oral dose of 2500 mg/kg bw/day (for 84 days) (van der Zande et al. 2014), a dose level that exceeds the current recommendations in OECD guidelines by a factor of 2.5. If taken up by cells, SAS particles are usually located in vesicles and endocytic compartments, and also along actin fibres and nuclear invaginations (Tarantini et al. 2015b); they are, however, never found in the nucleus. Reports to the contrary always refer to dye-labelled or otherwise modified silicon dioxide. Such materials, however, are not permitted for use as a food additive. These results cannot be read across to E 551, and it is not appropriate to conclude from them that unlabelled and unmodified silica particles would translocate in the cell nucleus. Several researchers have also relied on dye-labelled or modified silicon dioxide particles to trace the fate of silica in the body; these particles are, however, different from E 551 with regard to their physico-chemical properties (in particular different particle sizes, surface area, porosities, and stabilities), and the influence of dyes on the biodistribution, stability, and toxicity of silica particles is not sufficiently studied. Data generated from studies with these engineered materials cannot therefore be read across to E 551. Often results from intravenous studies are employed to derive biodistribution of SAS after oral exposure, e.g. by van Kesteren et al. (2015). Patterns of distribution can, however, be expected to be different, because of the different mode of application (local bolus directly into the intravenous compartment versus a slow absorption pattern by the large area of the intestinal tract after oral exposure), resulting also in different surface modifications (different corona formation) according to the route of entry (Inlivetox 2012). As already suggested earlier (NANOGENOTOX 2013), it can be concluded that the systemic availability of silica particles is low after oral exposure. The dissolved form might be absorbed by the intestinal tract.

Toxicity

Core areas defined by EFSA for the assessment of food additive toxicity include genotoxicity, subchronic and chronic toxicity, carcinogenicity, and reproductive and developmental toxicity as well as neurotoxicity, immunotoxicity, and endocrine-mediated effects (EFSA 2012). These areas are therefore addressed in the following sections.

Genotoxicity

Food-grade silicon dioxide was neither mutagenic nor genotoxic in standard in vitro test systems (Ames, HPRT, mouse lymphoma, and chromosome aberration studies) (CEFIC 2012a, b; ECETOC 2006). In vitro micronuclei tests were negative in human lymphocytes and BEAS2B cells, inconclusive in undifferentiated Caco-2 cells, and positive in some lung-derived cells at cytotoxic concentrations (NANOGENOTOX 2013; Tarantini et al. 2015b; Tavares et al. 2014; Zijno et al. 2016). The findings of in vitro indicator tests were negative for pyrogenic and hydrated silica under non-cytotoxic conditions (cf. Table 3 for details).

Table 3

Genotoxicity of silica in vitro (including data of non-food-grade and colloidal SAS)

Test system	Test substance	Particle size and/or SSA	Source	Method/treatment/parameters studied	Results	References
In gene mutation—bacteria
S. typhimurium TA98, TA100, TA1535, TA1537, TA1538	Pyrogenic SAS (CAB-O-SIL® EH-5)	385 m2/g	Cabot	Standard plate, agar plate, only with S-9	Negative	ECETOC (2006)
S. typhimurium TA98, TA100, TA1535, TA1537, TA1538	Pyrogenic SAS (CAB-O-SIL® M-5)	200 m2/g	Cabot	nr, ± S-9 (not defined)	Negative
S. typhimurium TA98, TA100, TA1535, TA1537, TA1538	Silica gel (Silcron G-910)	nr	nr	Standard plate, agar plate	Negative
Escherichia coli WP2	Silica gel (Silcron G-910)	nr	nr	Standard plate, agar plate	Negative
S. typhimurium TA1530, G-46	Silica gel (Syloid 244)	2.5–3.7 µm	nr	Spot test	Negative
S. typhimurium TA98, TA100, TA1535, TA1537, WP2uvrA	Colloidal silica*	20, 100 nm	E&B Nanotech Co Ltd	OECD TG 471, GLP	Negative	Kwon et al. (2014)
Gene mutation—mammalian cells
CHO cells	Pyrogenic SAS (CAB-O-SIL® EH-5)	385 m2/g	Cabot	OECD TG 476, GLP	Negative	ECETOC (2006)
L5178Y mouse lymphoma cells	Precipitated SAS (NM-200)	190 m2/g	JRC	OECD TG 476, GLP	Negative	CEFIC (2012a)
L5178Y mouse lymphoma cells	Pyrogenic and precipitated SAS (NM-200, -201, -202, -203)	10–22 nm	JRC	OECD TG 476	Negative	NANOGENOTOX (2013)
V79 hamster lung fibroblasts, HPRT	2 pyrogenic SAS, 1 precipitated SAS, 2 precipitated colloids*	20 and 25/70 nm (pyrogenic), 20 nm (precip.), 15, 40/80 nm (colloid)/50–200 m2/g	Commercial	12.5, 25, 50, 100 mg/L, 24 h	Negative	Guichard et al. (2015a)
Mouse embryonic fibroblast (MEF-LacZ cells)	Stöber silica without stabiliser*	10, 30, 80, 400 nm (actual sizes 11, 34, 34 and 248 nm); SSA nr	Glantreo	4, 40, 400 mg/L, 16 h	10, 80, 400 nm: negative30 nm: dose related ↑(2-threefold) at 40, 100, 400 mg/L; ↓viability (80 % at 85 mg/L): no particles in nucleus, implying secondary effect	Park et al. (2011)
Mouse lung epithelial (FE1) cells derived from Muta™ Mouse	Colloidal SAS* and 2 µm SAS	12, 5–15, 10–20 nm, 2 µm	Sigma-Aldrich, NIST	12.5 mg/L for 8 consecutive times	Negative, concentrations higher than 12.5 mg/L were cytotoxic and not included in the analysis	Decan et al. (2016)
In vitro micronuclei induction
BEAS2B, 16HBE, A549, Caco-2	Pyrogenic and precipitated SAS (NM-200, -201, -202, -203)	10–22 nm	JRC	OECD TG 487, 24 h, then cytB added	A549: positive for NM-201 and NM-202;Caco-2: inconclusive (1 exp positive, 1 exp negative); BEAS2B, 16HBE: mostly negative	NANOGENOTOX (2013)
BEAS2B	Precipitated silica (NM-200) and pyrogenic silica (NM-203)	22 nm, 190 m2/g	JRC	OECD TG 487, 0.1–100 mg/L, 48 h, cytB added 6 h after the beginning of treatment	Negative	Zijno et al. (2016)
Caco-2	Colloidal silica (Levasil® 50, Levasil® 200)*	15, 55 nm	HC Starck	OECD TG 487, 24 h treatment, then cytB added	15 nm: 1.5-fold↑ at 16 µg/mL and threefold↑ at 32 and 64 µg/mL in the presence of ↓RI (remained above 55 %); addition of FCS reduced effect by 50 %55 nm: no effect; addition of	Tarantini et al. (2015b)
Human lymphocytes	Pyrogenic and precipitated SAS (NM-200, -201, -202, -203)	10–22 nm, 160–230 m2/g	JRC	OECD TG 487, up to 1,250 mg/L, 24 h	Negative	Tavares et al. (2014)
Human lymphocytes	Colloidal silica (Levasil® 50, Levasil® 200)*	15, 55 nm/200, 50 m2/g	HC Starck	OECD TG 487, 31.6–1000 mg/L	Negative	Downs et al. (2012)
Balb/3T3 mouse fibroblasts	Precipitated silica (NM-200) and pyrogenic silica (NM-203); andcolloidal silica*	5–90 nm/50–200 m2/g	JRC	OECD TG 487, 100 mg/L, 24 h, then cytB added	Negative	Uboldi et al. (2012)
V79 hamster lung fibroblasts	2 pyrogenic, 1 precipitated, and 2 precipitated colloids*	20 and 25/70 nm (pyrogenic), 20 nm (precip.), 15, 40/80 nm (colloid)/50–200 m2/g	Commercial	12.5, 25, 50, 100 mg/L, 24 h	Negative	Guichard et al. (2015)
V79 hamster lung fibroblasts	Silica gel (Spherisorb® 5 µm)*	nr	nr	24 h, 20–160 µg/cm2	Weak but significant induction of micronuclei at cytotoxic doses	Liu et al. (1996)
Mouse embryonic fibroblast (MEF-LacZ cells)	Stöber silica without stabiliser*	10, 30, 80, 400 nm (actual sizes 11, 34, 34 and 248 nm); SSA nr	Glantreo	4, 40, 400 mg/L, 16 h	10, 30, 400 nm: negative80 nm: positive; no particles in nucleus, implying secondary effect	Park et al. (2011)
A549 human epithelial lung carcinoma cells	Stöber silica*	12–174 nm	Laboratory	OECD TG 487; 40 h	No significant induction of micronuclei; other weak chromosomal effects were observed, but again without reaching statistical significance; no cytotoxicity	Gonzalez et al. (2010, 2014)
In vitro chromosome aberration studies
Chromosome aberrations, CHO cells	Pyrogenic SAS (CAB-O-SIL® EH-5)	385 m2/g	Cabot	Equivalent to OECD TG 473, GLP-S9: 16 h, 38–300 µg/ml;+S9: 2 h, 250–1000 µg/mL	Negative	Cabot 1990 as cited in ECETOC (2006)
Chromosome aberrations,V79 cells	Precipitated silica (NM-200)	190 m2/g	JRC	OECD TG 473, GLP	Negative	CEFIC (2012b)
Chromosome aberrations,V79 cells	Colloidal silica*	20, 100 nm	E&B Nanotech Co Ltd	OECD TG 473, GLP	Negative	Kwon et al. (2014)
Chromosome aberrations, human embryonic lung cells (Wi-38)	Micronized silica gel (Syloid® 244)	2.5–3.7 µm	nr	24 h (presumably), only in the absence of S9, 1–1000 µg/mL	Negative	US-FDA 1974 as cited in ECETOC (2006)
In vitro UDS assays
Primary rat hepatocytes	Pyrogenic SAS (CAB-O-SIL® EH-5)	385 m2/g	Cabot	0.3–1000 µg/ml, with and without S9, exposure time 18–20 h	Negative	Cabot 1989 as cited in ECETOC (2006)
In vitro comet assays
HT-29 human colon carcinoma cell line	Pyrogenic SAS (AEROSIL® 200, AEROSIL® Ox50)	12, 40 nm, 200, 50 m2/g	Evonik Industries	Cytotoxicity (±FCS, 1 and 10 %, 0.03–156.3 µg/cm2); comet assay with and without Fpg	Negative, no oxidative DNA damage	Gehrke et al. (2013)
Human Caco-2 intestinal cells (undifferentiated)	Pyrogenic silica*	14 nm, SSA 200 m2/g	Sigma	20, 80 µg/cm2; 4 and 24 h, cytotoxicity (LDH and WST-1); 20 µg/cm2 for Fpg–comet assay (4 h treatment); glutathione	Positive (20 µg/cm2) in the presence of cytotoxicity (cytotoxic at 20 µg/cm2 after 24 (LDH) or 4 h (WST-1); DNA damage only with Fpg; ↓glutathione	Gerloff (2010) and Gerloff et al. (2009)
A549, HT29, and HaCat	Colloidal silica (Ludox SM-30)*	14 nm, agglomerated to 500 nm in medium	Sigma	24 h, 0.01–10 µg/mL	Significant increases in DNA damage at ≥0.1 mg/L in all tested cell types; cytotoxicity ≥1 mg/L	Mu et al. (2012)
3T3-L1 fibroblasts	Colloidal silica (LUDOX® CL and CL-X and non-stabilised SAS particles)*	20, 30, 80, 400 nm	Commercial and laboratory	4 and 40 μg/mL in DMEM; 3, 6, and 24 h incubation	Negative	Barnes et al. (2008)
SH-SY5Y neuronal cell line	Colloidal silica (LUDOX® AS-20, CL and AM, polygon)*	12 nm (nominal)	Commercial	48 h, up to 1000 ppm	Inconclusive	Kim et al. (2010)
Primary rat alveolar macrophages	Precipitated silica (NM-200)	230 m2/g	JRC	4 and 24 h incubation; 0, 0.01, 0.05, 0.25, 2.5, and 10 (10 only for 4 h incubations) µg/cm2; positive control D12 (25 µg/cm2; 4 h)	Negative, no oxidative DNA lesions; cytotoxic at highest dose level tested	CEFIC (2012c),
A549	Colloidal silica (Levasil®)*	9, 15, 30, 55 nm	AkzoNobel	Alkaline unwinding, 100–300 µg/mL	30, 55 nm: >50 µg/mL DNA damage; 9, 15 nm: at higher concentrations, at 100 µg/mL oxidative damage	Maser et al. (2015)
Rat, lung	Colloidal silica (Levasil®)*	15, 55 nm	AkzoNobel	Precision cut slices, 10–300 µg/mL	15, 55 nm: >100 µg/mL DNA damage; no overt cytotoxicity	Maser et al. (2015)
V79 hamster lung fibroblasts	Colloidal silica (Levasil®)*	15, 55 nm	AkzoNobel	Alkaline comet assay, alkaline unwinding assay,100–300 µg/mL, ± Fpg	15 nm: ↑strand breaks at 100 µg/mL (>twofold); no oxidative damage; no cytotoxicity55 nm: ↑strand breaks at 300 µg/mL (>twofold); no oxidative damage; no cytotoxicity	Maser et al. (2015)
V79 hamster lung fibroblasts	Silica gel (Spherisorb®)*	5 µm	Commercial	3 h treatment	Positive at ≥68.9 µg/cm2	Zhong et al. (1997)
V79 hamster lung fibroblasts	2 pyrogenic, 1 precipitated, and 2 precipitated colloids*	20 and 25/70 nm (pyrogenic), 20 nm (precip.), 15, 40/80 nm (colloid)/50–200 m2/g	Commercial	12.5, 25, 50, 100 mg/L, 24 h, comet assay with and without Fpg	Positive only with Pyr20 and Col15 in the presence of cytotoxicity and with Fpg, but no change in ROS; indicating indirect mechanisms	Guichard et al. (2015)
Phosphorylated gamma-H2Ax foci
Caco-2 cell line	Colloidal silica (Levasil® 50, Levasil® 200)*	15, 55 nm	HC Starck	Phosphorylated gamma-H2Ax foci, 24 h, 4–64 µg/mL/1.25–20 µg/cm2	15 nm: threefold↑ at 32 µg/mL and fivefold↑ at 64 µg/mL; likely a result of apoptosis as the caspase was also↑55 nm: no effect	Tarantini et al. (2015b)
Human HT-29 intestinal epithelial cell line	Mesoporous silica, core dye doped with two different labels*	25, 100 nm	Laboratory	Phosphorylated gamma-H2Ax foci, 24 h, 10, 50, 150 µg/mL	25 nm: 10, 50 (↑), 150 ↑100 nm: 10, 50 ↑, 150 no effect	Sergent et al. (2012)

cytB Cytochalasin B, HPRT hypoxanthine guanine phosphoribosyltransferase, NIST National Institute of Standards and Technology (USA), ROS reactive oxygen species

* Substance which does not fulfil the current EU criteria for E 551 (no star does, however, not implicate that the substance would be in compliance with EU E 551 specifications)

Standard oral in vivo genotoxicity tests were all negative (bone marrow micronucleus test, chromosome aberration test (NANOGENOTOX 2013; Tarantini et al. 2015a). Also, the pig-a test and the in vivo comet assay were negative (Guichard et al. 2015; NANOGENOTOX 2013; NANoREG 2015; Tarantini et al. 2015a). The organs/tissues studied in the comet assay were liver, kidney, blood, bone marrow, stomach, duodenum, and colon. It is noted that “significant increased DNA damage” in the comet assay was reported in bone marrow and spleen of male rats, and in ovary cells, all without a dose–response relationship, after daily gavage exposure for 45 days within a 90-day study with pyrogenic food-grade SAS. The low doses employed (maximum dose 50 mg/kg bw/day) which are below the normal range of rat diet silicon content (see, for example, Jugdaohsingh et al. 2015; van der Zande et al. 2014), the lack of a dose–response, and the known variability in comet results indicate that these observations are within the normal physiological range. No effects were found in testes, liver, kidney, intestines, and colon (Fessard et al. 2016; NANoREG 2015). Additionally, a non -standard colon micronucleus test was performed following repeated oral administration of precipitated and pyrogenic SAS on three consecutive days. No induction of micronuclei was found with precipitated SAS, but a slight increase in borderline statistical significance was noted in colon samples of rats treated with the lowest dose (5 mg/kg bw/day) of pyrogenic SAS (NANOGENOTOX 2013; Tarantini et al. 2015a). The preliminary genotoxicity data from the ongoing 90-day study in rats do, however, not show any genotoxic effects on the gastrointestinal tract (NANoREG 2015); in particular, no micronuclei were found in colon samples (Fessard et al. 2016) again indicating that the observations are within the normal physiological range and not substance related. The very low genotoxic potential is also evidences by studies employing the intravenous route, when genotoxicity (micronuclei) was only found at doses exceeding the maximum tolerated dose (MTD) level (Downs et al. 2012; NANOGENOTOX 2013).

The available genotoxicity studies are summarised in Table 3 (in vitro studies) and Table 4 (in vivo studies).

Table 4

Genotoxicity of silica in vivo (including data of non-food-grade and colloidal SAS)

Species, exposure route, dose levels	Test substance	particle size and/or SSA	Source	Method	Result	References
Ex vivo gene mutation tests
F-344 rat, inhalation, 50 mg/m3, 13 weeks	Pyrogenica silica (AEROSIL® 200)	200 m2/g	Degussa	HPRT mutations in alveolar type II cells	Negative, highly cytotoxic	Johnston et al. (2000)
In vivo micronucleus tests
Sprague–Dawley rat, m (up to 5/group); gavage, 5, 10, 20 mg/kg bw/day on 3 consecutive days	Pyrogenic silica (NM-202, -203)precipitated silica (NM-200, -201)	10–22 nm	JRC	OECD TG 474 (1997) (combined with comet assay), bone marrow	Negative	Nanogenotox (2013), (NANOGENOTOX (2013) and Tarantini et al. (2015a)
				Colon micronucleus assay (combined with comet assay)	Negative (NM-200, -201),borderline at lowest dose (NM-202,-203)
I CR mouse, gavage, 500, 1000, 2000 mg/kg bw, in 10 mL distilled water/kg	Colloidal silica*	20, 100 nm	E&B Nanotech Co Ltd	OECD TG 474 (1997), GLP; bone marrow	Negative	Kwon et al. (2014)
Sprague–Dawley rat, m (up to 5/group); 3, 6, 12 mg/kg bw/day by intratracheal instillation and 5, 10, 20 mg/kg bw/day by iv injection (iv only NM-203); on 3 consecutive days	Pyrogenic and precipitated silica (NM-200, -201, -202, -203)	10–22 nm	JRC	OECD TG 474 (1997) (combined with comet assay), bone marrow	Negative (intratracheal instillation)equivocal (iv, at highest dose corresponding to LD50)	Nanogenotox (2013), NANOGENOTOX (2013) and Guichard et al. (2015b)
Wistar rat, m, f, inhalation 1, 5, 25 mg/m3, 14 day + 14 day recovery	Precipitated silica (NM-200)	190 m2/g	JRC	OECD TG 474, polychromatic bone marrow erythrocytes, GLP	Negative	Knebel et al. (2014)
CR rat, m (5/group), inhalation (nose only), 7x10e7 and 1.8x10e8 particles/cm3 (1.8 and 86 mg/m3) for 1 or 3 day	Pyrogenic silica, de novo synthesised*	37, 83 nm	Laboratory	Micronucleus assay in peripheral blood cells by flow cytometry; lung pathology and inflammatory parameters	Negative, no adverse effects on lung, no inflammation	Sayes et al. (2010)
Wistar rat, m (4–8/group), iv, 25, 50, 125 (55 nm only) mg/kg bw/day for 3 days	Colloidal silica (Levasil® 200, Levasil® 50)*	15, 55 nm; 200, 50 m2/g	HC Starck	Combined micronucleus/comet assay; micronuclei in peripheral blood; test substance diluted and neutralised before injection into tail vein	Small increase in micronucleated reticulocytes at MTD, but not at lower doses	Downs et al. (2012)
In vivo chromosome aberration
Sprague–Dawley rat, m (1 and 5 × 1.4–5000 mg/kg bw, oral)	Silica gel(Syloid® 244)	2.5–3.7 µm	nr	Chromosome aberration in bone marrow cells; animals killed 6, 24, or 48 h after single administration or 6 h after last administration in the repeated-dose experiment	Negative	US-FDA 1974 as cited in ECETOC (2006)
In vivo comet assays
Sprague–Dawley rat, m (up to 5/group); 5, 10, 20 mg/kg bw/day by gavage and iv (iv only for NM-203); up to 12 mg/kg bw/day by instillation; on 3 consecutive days	Pyrogenic and precipitated silica (NM-200, -201, -202, -203)	10–22 nm	JRC	Combined comet/micronucleus assay; liver, kidney, blood, bone marrow; for the oral route in addition: duodenum and colon;For the instillation route in addition: lung, BAL fluid.	Negative (in all organs and tissues), no overt toxicity except for iv route (LD50)	Nanogenotox (2013), Tarantini et al. (2015a), and Guichard et al. (2015b)
Sprague–Dawley rat, gavage, 500, 1000, 2000 mg/kg bw, at 0, 24 and 45 h before killing	Colloidal silica*	20, 100 nm	E&B Nanotech Co Ltd	OECD TG 489, GLP; liver, stomach	Negative	Kwon et al. (2014)
Wistar rat, m,f, inhalation 1, 5, 25 mg/m3, 14 day + 14 day recovery	Precipitated silica (NM-200)	190 m2/g	JRC	Ex vivo comet assay (± hGOOG1) in alveolar macrophages from BAL; immunohistochemistry in lung epithelial cells	Macrophages: small, concentration-dependent increase in DNA damage, particularly after the recovery period; no oxidative damage; particle aggregates/agglomerates in cytoplasm of intraalveolar macrophages; in lung epithelial cells slight, but significant increase in 8-OH-dG positive nuclei at d1 and d14 post-exposure	Knebel et al. (2014)
Wistar rat, m (4–8/group), iv, 25, 50, 125 (55 nm only) mg/kg bw/day for 3 days	Colloidal silica* (Levasil® 200, Levasil® 50)	15, 55 nm; 200, 50 m2/g	HC Starck	Combined comet/micronucleus assay; test substance diluted and neutralised before injection into tail vein; organs examined: liver, lung, white blood cells	15 nm: small increase in DNA damage at 50 mg/kg bw55 nm: ↑DNA damage (1.5–1.7 ×) at 125 mg/kg in liver (MTD)	Downs et al. (2012)
Rat, single intratracheal instillation, 360 µg	Colloidal silica* (Levasil®)	15, 55 nm;	Akzo Nobel	Lung, bone marrow, 3 day after instillation	Negative, pulmonary inflammation (more pronounced with 15 nm)	Maser et al. (2015)
Drosophila tests
Drosophila melanogaster	Colloidal silica* (Levasil®)	6, 15, 30, 55 nm and micron-sized, 50–450 m2/g	HC Starck	Wing-spot and comet assay (± FPG) in larvae haemocytes: larvae were fed 0.1–10 mM	Negative (no significant increases in the frequencies of somatic and recombination mutants);>5 mM: DNA damage (both ± FPG)	Demir et al. (2015)

BAL bronchoalveolar lavage, FPG formamido pyrimidine glycosylase, HPRT hypoxanthine phosphoribosyltransferase-encoding gene mutation, iv intravenous, m male, MTD maximum tolerated dose, nr not reported)

* Substance which does not fulfil the current EU criteria for E 551 (no star does, however, not implicate that the substance would be in compliance with EU E 551 specifications)

aIn publication erroneously described as “precipitated silica”

Subchronic and chronic toxicity

The available oral studies include 28-day gavage and feeding studies with pyrogenic silica, a 28-day gavage study with precipitated silica (CEFIC 2011; van der Zande et al. 2014), an old 90-day feeding study with pyrogenic silica (ECETOC 2006), and 84- and 90-day feeding studies with precipitated silica (ECETOC 2006; van der Zande et al. 2014). A low-dose 90-day gavage study with pyrogenic silica (NM-203) is still ongoing (NANoREG 2015). Details on the older studies have been summarised in the documents by the IARC (IARC 1997), the OECD (OECD 2004), the ECETOC (ECETOC 2006), the EFSA (EFSA 2004, 2009) and been published within the OECD Programme on Nanomaterials.1

An overview of the available oral studies and their main results is presented in Table 5. Because colloidal silica is chemically identical apart from stabilisers and possibly additives, results with colloidal silica are also included in the table, although it is not permitted to be used as E 551.

Table 5

Repeated-dose oral toxicity studies in rodents with food-grade SAS (including colloidal silica)

Substance, SSAa	No. and sex/group	Exposure	Dose levels	Effects	References
Pyrogenic silica
Rat
NM-203	5 m, 5f	90 day, gavage	0, 2, 5, 10, 20, or 50 mg/kg bw/day; silicic acid (50 mg/kg bw/day) as comparator	Preliminary data: alterations in blood count, PHA-induced lymphocyte proliferation (spleen, MLN), and LPS-induced NO and cytokine production (peritoneal resident macrophages). Clinical biochemistry: no alterations in liver biomarkers, but alterations in creatinine in males at ≥10 mg/kg and in females at ≥2 mg/kg	NANoREG (2015)
Food-grade silica, 380 m2/g	5 m	28 day (high dose also 84 day), diet	100, 1000, 2500 mg/kg bw/day	28 day: no effects except small but significant increase in villus heights and crypt depths, but no significant differences in the ratio between the villus height and crypt depth; 84 day: no significant effects; ↑Si in spleen	van der Zande et al. (2014)
NM-202, 200 m2/g	5 m	28 day (high dose also 84 day), diet	100, 500, 1000 mg/kg bw/day	28 day: no effects except small but significant increase in villus heights and crypt depths, but no significant differences in the ratio between the villus height and crypt depth; 84 day: ↑periportal fibrosis in the liver; induced gene expression in a fibrosis-related gene set	van der Zande et al. (2014)
Pyrogenic silica, SSA nr	Rat	90 day, gavage and diet	Up to 100 mg/kg bw/day	No toxic effects	Shumakova et al. (2014a, b, 2015)
HDK® V15, 130–170 m2/g	20 rats	5 day/week for 4 weeks (gavage)	0 (vehicle control); 500 mg/kg bw in water	No effects on appearance, behaviour, food consumption, and body weight	Klosterkoetter (1969)
CAB-O-SIL® Fluffy (very similar to CAB-O-SIL® M5), 200 m2/g	15 m, 15f	90 day (in diet)	0, 1, 3, 5 % in diet (ca. 0, 1000, 3000, 5000 mg/kg bw/day); 3 % cosmetic talc as positive control	No signs of systemic toxicity, no effect on growth rate, food consumption, or survival; no gross or microscopic pathological changes	Cabot 1958 as cited in ECETOC (2006)
Hydrated silica
Rat
NM-200, 230 m2/g	5 m	28 day + 14 day recovery (gavage)	0, 100, 300, 1000 mg/kg bw/day	NOEL 1000 mg/kg bw/day, GLP	CEFIC (2011)
Levasil® 200, 40 % dispersion*	5 m, 5f	28 day	0, 1000 mg/kg bw/day	NOEL 1000 mg/kg bw/day	Buesen et al. (2014)
Colloidal silica (20 and 100 nm)*	10–15/sex	90 day (gavage)	0, 500, 1000, 2000 mg/kg bw/day	NOEL 2000 mg/kg bw/day, GLP	Kim et al. (2014)
Sipernat®22, 190 m2/g	10 m, 10f	90 day (in diet)	0, 0.5. 2, 8 % in diet (ca. 0, 250, 1000, 4000 mg/kg bw/day)	No effects at 250 and 1000; at 4000: increased food intake with decreased food efficiency, abs, and rel. caecum weights↑ (not a toxic effect); no gross or histopathological changes	Degussa 1981, as cited in ECETOC (2006)
Syloid® 244	5 m, 5f	14 day (in diet)	0, 5–20 % in diet (5 % day 1–10, 20 % day 11–14)	No clinical signs, no effects on food or water consumption, bw gain, behaviour	Grace, 1974 as cited in ECETOC (2006)
Syloid® 244	5 m, 5f	6 m (in diet)	0, 3.2, 10 % in diet (m: 2170, 7950 mg/kg bw/day; f: 2420, 8980 mg/kg bw/day)	No clinical signs, no effects on food or water consumption, bw gain, behaviour, survival, haematology, clinical chemistry; no gross pathological or microscopic findings	Grace 1975 as cited in ECETOC (2006)
Syloid® 244	20 m, 20f	103 wk (in diet)	0, 1.25, 2.5, 5 % in diet; the top dose corresponds to 2500 mg/kg bw/day	No effects on bw, food consumption, clinical signs, clinical chemistry; no gross or microscopic findings. Liver weights↓ (f) in mid- and high-dose groups. The OECD derived from this study a NOAEL for chronic oral administration at approximately 2500 mg/kg bw/day	Takizawa et al. (1988), EFSA (2004 and OECD (2004)
Mouse
Syloid® 244	20 m, 20f(B6C3F1)	Up to 21 months (in diet)	0, 1.25, 2.5, 5 % in diet; the top dose corresponds to 7500 mg/kg bw/day	7000 mg/kg bw/day: ↓growth, no other effects; no gross or microscopic findings; occurrence of tumours not different from controls	EFSA (2004), OECD (2004) and Takizawa et al. (1988)

* Substance which does not fulfil the current EU criteria for E 551 (no star does, however, not necessarily implicate that the substance would be in compliance with EU E 551 specifications)

aSSA specific surface area

In the 28-day rat studies, NOAELs of 1000 mg/kg bw/day or above were determined, with no specific target organs identified (CEFIC 2011; van der Zande et al. 2014). In an extended study according to OECD TG 407 (CEFIC 2011), the measurements of the spontaneous locomotor activity and the functional observational battery showed no influence of the treatment up to and including the highest tested dose of 1000 mg/kg bw/day (it is noted that only male animals were used in this study).

Similarly, the NOAELs in the 90-day rat studies were also at 1000 mg/kg bw/day or above with no specific target organs identified (ECETOC 2006; van der Zande et al. 2014). Only in one single rat study, in which two pyrogenic silica products (designated NM-202 and “SAS”) were studied in parallel, mild liver fibrosis was reported after oral exposure to 1000 mg NM-202/kg bw/day, but not after dosing SAS at 2500 mg/kg bw/day. In this study (van der Zande et al. 2014), the occurrence—but not the severity—of periportal liver fibrosis was reported to be significantly increased (p = 0.021 when compared with the control animals). Fibrosis is, however, not visible on the histological images presented in the publication [Fig. 5, F/G; referred to as showing fibrosis by (van der Zande et al. 2014)]. The small amount of connective fibrous tissue represents the normal anatomical situation of periportal fields where bile ducts, arteries, and veins are located within a small amount of fibrous tissue. Hence, the histopathological diagnosis of periportal fibrosis in this study has to be questioned (K. Weber, AnaPath GmbH, personal communication, July 2016). Liver silicon concentrations, liver enzymes, and biochemical markers were unaffected, and “the number of lymphocytic cells and thereby also the number of inflammatory granulomatous foci (the average number of cells in each of the foci) remained unchanged after 28, and 84-days” was unchanged, as evidenced by quantitative histological assessment of livers (van der Zande et al. 2014). There was therefore no evidence found for Kupffer cell activation (which might induce liver re-modelling), or inflammatory granuloma formation as a key event for the induction of liver fibrosis. Results from in vitro studies show that liver cells are not very sensitive towards SAS, and that relevant toxicity is only caused when incubated with Kupffer cells (Chen et al. 2013). Taken together, the lack of significant exposure after oral intake and the absence of inflammation, resident or peripheral macrophage stimulation and granuloma formation in the liver, clearly demonstrate that there is no plausible mechanism for the induction of liver fibrosis by oral intake of E 551.

Carcinogenicity

The carcinogenic potential of silica gel (Syloid® 244) was investigated in an oral study in B6C3F1 mice and F344 rats (20/sex/group/species) after dietary administration at 0, 1.25, 2.5, and 5 % for at least 21 and 24 months, respectively. These concentrations correspond to dose levels of approximately 0, 1800, 3500, and 7000 mg/kg bw/day for mice and 0, 625, 1250, and 2500 mg/kg bw/day for rats. The administration of silica gel was well tolerated. There were no biologically or toxicologically significant changes, and silica gel was therefore considered to be devoid of toxic or carcinogenic potential (Takizawa et al. 1988).

Reproductive and developmental toxicity

No adverse effects on reproduction or development were found in old and limited studies with silica gel as reviewed by the EFSA (2004, 2009). In a dominant lethal test performed with silica gel [US-FDA 1974, as cited by ECETOC (2006)], no effects of treatment (up to 5 × 5000 mg/kg bw, oral) were found on fertility index, total number of implantations of corpora lutea, pre-implantation losses, and dead implants. Results from several oral repeated-dose toxicity studies did not indicate any adverse health effects including the highest tested dose levels.

After intravenous dosing of mice with colloidal 70-nm silica particles, placental dysfunction, foetal resorption, and growth retardation were reported (0.2, 0.4 and 0.8 mg/mouse on gestational day (GD)16 and GD17) (Yamashita et al. 2011). The effects could be prevented by a combined treatment with heparin, and the activation of the complement system was therefore assumed as a potential mechanism. The test material is different from E 551, and it is unlikely that after oral exposure, silica particles would be present in blood and reproductive organs at similarly high levels as achieved by intravenous injection. These findings are therefore not relevant for the assessment of E 551. Modern guidelines studies employing the oral route have not shown any adverse effects of food-grade silicon dioxide on the foetus (see below).

In a recent embryonic stem cell test (EST) performed within the EU FP7-funded MARINA project, precipitated and pyrogenic silicon dioxide as used in food additives (NM-200 and NM-203) was classified as “non-embryotoxic” (Farcal et al. 2015). A study reporting inhibition of embryonic stem (ES) cell differentiation after exposing the D3 murine ES cell line to spherical laboratory-synthesised amorphous silica nanoparticles (Park et al. 2009) is not considered relevant, as such material is not used in food additives. In vitro studies with human placental models, performed within the EU FP7 project NanoTest, were inconclusive, and, as it seems performed with fluorescent dye-labelled material which is different from E 551 (Dusinska and Tran 2015; Poulsen et al. 2015).

Within the Cefic LRI testing programme, precipitated synthetic amorphous silica has recently been tested in GLP and guideline studies for its potential effects on reproduction and development. These studies are summarised in the following sections.

A GLP oral two-generation reproductive toxicity study was recently performed in the Wistar rat according to OECD guideline 416 (Wolterbeek et al. 2015). The administration by gavage of precipitated synthetic amorphous silica (NM-200, purity 96.5 %) at doses up to 1000 mg/kg bw/day had no adverse effects on the reproductive performance of rats or on the growth and development of the offspring into adulthood for two consecutive generations. The NOAEL was 1000 mg/kg bw/day. The mean hydrodynamic diameter of the silica particles in the 10 g/L study samples (dispersion in 0.5% aqueous methylhydroxypropyl cellulose) varied between 1076 and 1664 nm and for the 30 g/L study samples between 876 and 1216 nm, respectively. The measured size of the 100 g/L study samples appeared to be the smallest (409–703 nm), but due to the high concentration of the particles in the samples, the particles sedimented and aggregated. The animals received the test substance during a premating period of 10 weeks, during mating, gestation and lactation until sacrifice. Dams were allowed to raise one litter. At the end of the lactation period, pups were weaned and selected for the next generation. F0- and F1-dams were sacrificed at or shortly after weaning, and F0- and F1-males after mating. F1-pups were dosed by gavage at the same dose levels as their parents from post-natal day 21 until sacrifice. Reproductive parameters (including oestrus cycle, evaluation, and sperm analysis) and developmental parameters (including sexual maturation) were measured. At sacrifice, reproductive organs and tissues were sampled for histopathological analysis. In both generations, no treatment-related effects were observed on body weights, body weight changes, and food consumption. In both generations, no treatment-related effects were observed on oestrus cycle parameters of the female animals and on sperm parameters of the male animals. In both generations, no effects of the test item were observed on mating, female fecundity, male- and female fertility, and gestation indices. Pre-coital time, duration of gestation, and the incidence of dams with stillborn pups or implantation loss were not affected. No test substance-related adverse effects were observed on the mean number of pups delivered, the incidences of live born- and stillborn pups, the number of pups lost during the lactation period, the sex ratio, clinical observations, nor necropsy findings. In both generations, there were no effects on pup weights and pup weight changes. No statistically significant differences were found amongst the various groups in timing of testes descent, preputial separation, and vaginal opening. There were no gross pathological findings in the stillborn pups, pups that died during lactation, and in pups necropsied at post-natal day 21. Organ weights of pups and of parents of both generations were not affected. There were also no gross or histopathological changes in the F0- and F1-generation animals.

In a GLP study on rats in compliance with OECD TG 414, orally administered precipitated silica (NM-200) had no adverse effects on development; the NOEL was at 1000 mg/kg bw/day, the highest dose tested (Hofmann et al. 2015; Schneider et al. 2011). The test substance, dispersed in water with 10 % foetal bovine serum, was administered at doses of 0, 100, 300, and 1000 mg/kg bw/day from GD 6 through GD 19 by oral gavage. On GD 20, all females were assessed by gross pathology, including weight determinations of the uterus and the placentas. The corpora lutea were counted, and the number and distribution of implants (resorptions, live and dead foetuses) were determined. The foetuses were removed from the uterus, their sex determined, weighed, and investigated for external findings. Half of the foetuses of each litter were examined for soft tissue findings and the remaining foetuses for skeletal (and cartilage) findings.

No differences were found between the treatment groups and controls with regard to clinical observations, body weights, and food consumption of the dams. No effects were observed on number of corpora lutea, implants, pre- and post-implantation losses, or number and viability of offspring. External, soft tissue and skeletal examination showed no test substance-related abnormalities. The No Observed Effect Level (NOEL) for maternal and developmental toxicity was therefore derived at 1000 mg/kg bw/day, the highest dose tested.

The results of this modern guideline study are in line with earlier findings in limited prenatal developmental toxicity studies performed with silica gel (Syloid® 244) by the oral route in rats, mice, hamsters, and rabbits. The NOELs for maternal and developmental toxicity in these studies were at 1340–1600 mg/kg bw/day, the highest doses tested (US-FDA 1973. as cited in ECETOC 2006).

Neurotoxicity, immunotoxicity, endocrine-mediated effects

The available information from repeated-dose, developmental, and reproductive toxicity studies does not indicate any adverse effects on the nervous, immune, or endocrine systems. Dedicated studies for neurotoxic, immunotoxic, or endocrine-mediated effects were therefore not performed. A functional observation battery (FOB) was integrated in the 28-day repeated-dose toxicity study (CEFIC 2011), and lymphoid tissue and immunological parameters were investigated in 28- and 84-day studies (van der Zande et al. 2014). In none of these investigations, was any indication of neurological or immunological effects found.

With two food-grade pyrogenic silicon dioxide products (NM-203 and SAS), no effects were found on antibody levels in blood (IgG and IgM), or on cytokine levels produced by proliferating T and B cells, that were isolated from spleen and mesenteric lymph nodes. Proliferation of the isolated T and B cells and the activity of NK cells isolated from spleen were also examined after 28 days of exposure, but remained unaffected. Immunotoxicity parameters were also studied in vitro and in vivo within the 90-day gavage study performed with NM-203 in the low-dose range (Di Felice et al. 2016; NANoREG 2015). The parameters studied in vitro included inflammatory and functional endpoints in the murine macrophage cell line RAW264.7, apoptosis/necrosis, cytokine secretion, and NO production. No significant effects were found in vitro, but an “impaired lymphocyte response to mitogen associated with reduced numbers of circulating white blood cells, and enhanced inflammatory response by peritoneal macrophages” were reported (Maranghi et al. 2016). In the absence of any other biochemical changes indicating inflammatory processes or adverse effects, these findings and the reported statistically significant increase in the incidence of intralobular lymphoid infiltration in livers of females are not considered to have toxicological relevance. Since the silica doses employed in this study are within the normal silica content range of usual laboratory rat diets*, it is very unlikely that an adverse effect would be caused by the doses employed. [*up to 5000 µg Si/g food ((De Jong 2012), resulting in up to 400 mg Si/kg bw assuming a food intake of 20 g/day for a 250 g rat.].

Information from in vitro studies

Many have investigated the in vitro effects of silica particles in a wide variety of cell types. While previously cells of the respiratory tract were the focus of the studies, more recently also cells relevant, or potentially relevant, for the oral exposure route have been investigated. E 551 exerts its function as anti-caking agent mainly by covering the surface of host powders and thus acting as a spacer, and through its water adsorption capacity. Similarly, SAS particles may adsorb to cellular surfaces and proteins and may thus affect biological membrane structures and integrity. The in vitro results vary with the cell type and the extent of direct contact with silica particles. Except for red blood cells, macrophages were shown to be the most sensitive cell type after in vitro exposure to pyrogenic and precipitated silica. Alveolar macrophages were more sensitive than peritoneal macrophages (Di Cristo et al. 2016; Farcal et al. 2015; Fruijtier-Pölloth 2012). This is consistent with the physiological function of macrophages to clear particulate matter from the body. In vitro studies in cell types with relevance to the oral exposure route are summarised in Table 6.

Table 6

In vitro studies assessing cytotoxicity, ROS production, and inflammatory effects of silica (including data of non-food-grade and colloidal SAS but excluding pulmonary system)

Cell system	Test substance	Particle size/SSA	Source	Treatment/parameters studied	Results	References
Liver
Human hepatic L-02 cell line	Colloidal SiO2*	21. 48 and 86 nm/225, 106, 39 m2/g	Center of Analysis and Test Research (East China, Shanghai)	200–1000 mg/L for 12, 24, 36, and 48 h; cytotoxicity (LDH release, MTT), ROS, and ultrastructure (TEM); glutathione, lipid peroxidation, apoptosis	≥400 mg/L: 21-nm particles were cytotoxic and induced oxidative stress, lipid peroxidation, GSH depletion, apoptosis, and upregulated levels of p53 and Bax-Bcl-2 ratio	Ye et al. (2010b)
Human HepG2 hepatoma cell line and L-02 cells	SAS (not specified in publication)	7, 20, 50 nm/380, 150, 65 m2/g	Shanghai Cabot Chemical Co Ltd	20, 40, 80, 160, 320, 640 mg/L;48 h; MTT, apoptosis, oxidative stress, p53, caspase-3, Bcl-2, procaspase-9, Bax	↓Viability at ≥80 mg/L in HepG2 and ≥320 mg/L in L-02 (7,20 nm); 50 nm: not cytotoxic; apoptosis and oxidative stress in HepG2 at ≥160 mg/L; possibly activation of mitochondrial-dependent pathway by oxidative stress; no effects in L-02 cells	Lu et al. (2011)
Human HepG2 hepatoma	SAS, purity 99.5 %	15 nm/640 m2/g	Nanostructured& Amorphous Materials, Inc. (Houston, TX)	1–200 mg/L, 72 h; MTT, NRU, ROS production, lipid peroxidation, GSH depletion; PCR, immunoblotting	↓Viability at ≥25 mg/L, oxidative stress and apoptosis; mRNA and protein expressions of cell cycle checkpoint gene p53 and apoptotic genes (bax and caspase-3) were upregulated while the anti-apoptotic gene bcl-2 was down-regulated; attenuated by vitamin C addition	Ahmad et al. (2012)
Human HepG2 hepatoma	Stöber silica*	20, 40, 60, 80, 100, 150, 200 nm	Laboratory	10–500 mg/L. 24, 72 h, viability, MTT, LDH	No size dependent changes in viability, except for highest dose of 60-nm particles, ↑LDH leakage at 500 mg/L, viability after 72 h not significantly reduced in comparison with 24 h of exposure	Kim et al. (2015)
Human HuH-7 cells	Not specified (“nanosilica”)*	10–20, 40–50, 90–110 nm, and 45 μm/SSA nr	Gift from Prof. Hee Kwon Chae	5 concentrations, (0.24 µg/L to 2.4 mg/L), 72 h; MTT, DNA content, GSH, oxidative stress	Smallest particles: 15 % increase in glutathione production; otherwise no size dependency; no other effects	Cha and Myung (2007)
Kupffer cells (KC); Buffalo rat liver cells (BRL) + supernatant from SiO2-treated KC	Colloidal silica*	15 nm, aggregated to 92 nm in culture medium (RPMI 1640)/SSA nr	Sigma	50–800 mg/L, 24 h, TNF-α and H2O2 in supernatant; LDH, AST in BRL cells	≥200 mg/L: KCs released NO; at ≥400 mg/L: ↑ROS, ↑TNF-α≥100 mg/L ↓viability of BRL cells with supernatants of SiO2 NP-stimulated KCs; ≥400 mg/L ↑AST	Chen et al. (2013)
Gastrointestinal tract
Human TR146 buccal epithelial cells	Not specified (“nanosilica”)*	14 nm/SSA nr	Sigma	0–1250 µM apoptosis, cell cycle, ROS production, inflammatory response	No effects on apoptosis and cell cycle; ≥500 µM: oxidative stress, ↑IL-6, ↑TNF-α	Tay et al. (2013)
Human MKN-1 stomach cells	Not specified (“nanosilica”)*	10–20, 40–50, 90–110 nm, and 45 μm, not specified/SSA nr	Gift from Prof. Hee Kwon Chae	5 concentrations, (0.24 µg/L to 2.4 mg/L); 72 h MTT, DNA content, GSH, oxidative stress	No effects	Cha and Myung (2007)
DLD-1, SW480 and NCM460 cells (G2/M synchronised)	Not specified (“nanosilica”)*	14 nm/SSA nr	Sigma	0, 62.5, 250, 1000 µM for 24 h, morphology, cell cycle, ROS production	Minimal biological responses from the intestinal cells	Setyawati et al. (2015)
Human gastric adenocarcinoma (MKN-28), human colon carcinoma (HT-29)	Precipitated from sodium silicate or TEOS*	21, 80 nm; hydrodynamic size 188.3 and 236.3/SSA nr	Laboratory	Up to 667 mg/L. 48 h, MTT, LDH	Slightly cytotoxic at high concentrations, IC20 in HT-29: 508–510 mg/L; MKN-28: 443–572 mg/L)	Chang et al. (2007)
Human Caco-2 intestinal cells	Pyrogenic SAS*	14 nm/SSA 200 m2/g	Sigma	5 µg/cm2; 4 and 24 h treatment; WST-1 assay with native and digestion simulated particle suspensions; both in undifferentiated and in differentiated cells	No effect of particle pre-treatment on cytotoxicity (WST-1 assay) or IL-8 expression; ↓viability only in undifferentiated cells after 24 h (IC50 9 and 10 µg/cm2 for pristine and pre-treated silica);	Gerloff et al. (2013)
Human Caco-2 intestinal cells	Pyrogenic silica (AEROSIL 300, 380), precipitated silica (Tixosil 43, 73)	300, 380 m2/g (pyrogenic), 8–10 µm (precipitated)	Evonik, Rhodia	100 mg/L, MTS, LDH	Only slightly cytotoxic (undifferentiated cells), independent of dispersion method (ultrasonication, mechanical stirring)	Contado et al. (2016)
Human Caco-2 intestinal cell line	Colloidal silica* (Levasil®)	15, 55 nm/200, 50 m2/g	HC Starck	4–256 µg/mL/1.25–80 µg/cm2; Cytotoxicity (XTT assay), caspase activity, intracellular ROS (DCFH-DA fluorescence); IL-8	55 nm:↓viability (30 %) at 256 µg/mL; no effect on ROS15 nm: IC50 43 µg/mL; at 64 µg/mL caspase↑; at 32 and 64 µg/mL fluorescence↑; at 32 µg/mL IL-8↑	Tarantini et al. (2015b)
Human Caco-2BBe1 cell line	SAS*	12 nm, 175–225 m2/g pristine or treated with digestive enzymes; purity 99.8 %	Sigma	10 µg/cm2, 24 h either single or repeated weekly; necrosis, apoptosis, membrane damage, and mitochondrial activity;	10 µg/cm2: no decrease in viability or mitochondrial activity; no significant toxicities after long-term treatment	McCracken (2015) and McCracken et al. (2013, 2016)
Human Caco-2BBe1 cell line	6 samples of pyrogenic SAS	9–26 nm/SSA nr	Commercial vendors in China and USA	0.01, 0.1, 1 mg/L medium, 24 h; ROS	“Brush border disruption”: up to 43 % loss of microvilli at 1 mg/L	Yang et al. (2016) [11]
Human gastric epithelial cell (GES-1), colorectal adenocarcinoma cells (Caco-2)	2 pyrogenic SAS (AEROSIL®), two precipitated SAS	151–357m2/g	Vendors in China	Up to 600 mg/L, 4–72 h, cytotoxicity (WST, LDH) cellular uptake, ROS, cell cycle, apoptosis,	100 mg/L: no effects, ≥200 mg/L cell cycle arrest and reduced cell growth, but no apoptosis or necrosis; no passage through Caco-2 cell layer (after 4 h)	Yang et al. (2014) [134]
Cardiovascular system
Red blood cells	Pyrogenic silica	200 m2/g	Cabot	30 min at RT with 40–2000 mg/L in Dulbecco’s phosphate-buffered saline	Silanol groups might be involved in the lysis process	Pandurangi et al. (1990)
Human red blood cells	Precipitated SAS (Zeofree 80, purity 98 %)	1–3 µm/77.7 m2/g	Commercial	Haemolysis of human RBCs (tested at 0.1–26.5 µg/cm2), neg control PBS, pos control 1 % Triton-X 100	Haemolysis at ≥0.1 µg/cm2	Sayes et al. (2007)
Human red blood cells	Stöber silica*, mesoporous silica*	115 nm (Stöber), 120 nm (mesoporous)	Laboratory	0–500 mg/L, 24 h	Nonporous Stöber silica showed highest cellular association; cellular association was directly linked to plasma membrane damage	Yu et al. (2011)
Rabbit red blood cells	Pyrogenic silica*, mesoporous silica*	16 nm (pyrogenic silica)/mesoporous silica: 1138 m2/g	Sigma-Aldrich	20, 40, 60 and 100 mg/L	Haemolysis related to the number ofsilanol groups accessible to the cell membranes of red blood cells	Slowing et al. (2009)
Mouse red blood cells	Pyrogenic silica (AEROSIL); Stöber silica*	16 nm (pyrogenic silica), nr for Stöber silica	Sigma; laboratory	25, 50, 100 mg/L, 2 h	Pyrogenic, but not Stöber silica caused haemolysis	Zhang et al. (2012)
Endothelial cell line EAHY926	Colloidal silica*	14, 15, 16, 19, and 60 nm monodisperse, spherical particles; 104, 335 nm	Laboratory	LDH, MTT	↓Cell viability, EC50: 33–47 µg/cm2 for 14,15, and 16 nm, 89 and 254 µg/cm2 for 19 and 60 nm; >1 mg/cm2 for 104 and 335 nm;	Napierska et al. (2009)
Myocardial H9c2(2–1) cells	Colloidal silica*	21 and 48 nm/225 and 106 m2/g	Center of Analysis and Test Research (East China. Shanghai)	100–1600 mg/L for 12, 24, 36, and 48 h; cytotoxicity (LDH release, MTT), ROS, glutathione, lipid peroxidation, apoptosis	↑Oxidative stress, lipid peroxidation, GSH depletion, apoptosis, and upregulated levels of p53 and Bax-Bcl-2 ratio	Ye et al. (2010a)
Primary human umbilical vein endothelial cells (HUVECs)	Stöber silica*	62 nm; Ru-labelled for cellular uptake studies	Laboratory	0, 25, 50, 75, 100 mg/L for 6, 12 and/or 24 h; LDH, MTT, ROS, apoptosis, Comet, cellular uptake, cell cycle arrest, mitochondrial membrane potential	≥25 mg/L: ↑LDH, necrosis, ↑ROS, cell cycle arrest; changes in mitochondrial membrane potential≥50 mg/L: ↑MTT(24 h), apoptosis, DNA damage	Duan et al. (2013)
Macrophages
Mouse peritoneal macrophage cell line RAW 264.7	SAS*	14 nm (SSA 77.7 m2/g); “mild” aggregation (not specified further)	Sigma	0.0052, 0.052, 0.52, 5.2, 52, and 520 µg/cm2). 24 h/cytotoxicity (MTT, LDH), apoptosis; serum-free medium	≥5.2 µg/cm2 cytotoxicity; 2x52 µg/cm2 for 24 h: apoptosis (DNA fragmentation); annexin V binding after 6 h	Kim et al. (2009)
RAW 264.7	SAS, purity 99.8 %*	12 nm/SSA nr	Commercial	5–40 ppm	ROS↑ intracellular GSH↓nitric oxide↑	Park and Park (2009)
RAW 264.7	Stöber silica*, mesoporous silica*	115 nm (Stöber), 120 nm (mesoporous)	Laboratory	100, 250, 500 mg/L, 24 h, WST-8	Cytotoxic at lowest dose tested (100 mg/L)	Yu et al. (2011)
RAW 264.7	NM-200, NM-203	14 nm (NM-200), 13 nm (NM-203)/189.2 m2/g and 203.9 m2/g	JRC	1–100 mg/L, 24, 48, 72 h viability (LDH)	NM-200: IC50 (72 h) >100 mg/L;NM-203: IC50 (72 h) 64–86 mg/L	Farcal et al. (2015)
RAW 264.7	NM-200, NM-203	189, 203 m2/g	JRC	2.5, 5, 10, 20, 40, 80 µg/cm2;24,48, 72 h	≥5 µg/m2: ↑inflammatory cytokines (at 10 µg/m2: NM-200 less than NM-203); more efficient cellular uptake of pyrogenic silica	Di Cristo et al. (2016)
Peripheral human blood mononuclear cells (PBMC)	Colloidal silica suspensions*	10, 100 nm	Polysciences, Inc.	50 (500 for 100 nm)-4000 mg/L, 24, 48 h	↓Viability at ≥50 mg/L (10 nm) or 500 mg/L (100 nm), GSH depletion and cytokine changes at ≥250 mg/L (10 nm) or 500–2000 mg/L (100 nm)	Mendoza et al. (2014)
Human acute monocytic leukaemia cells (THP-1)	Pyrogenic silica (AEROSIL); Stöber silica*	16 nm (pyrogenic silica), nr for Stöber silica	Sigma; laboratory	0.4–200 mg/L, 24 h, IL-1beta	≥50 mg/L: ↑IL-1β, activation of Nalp3 inflammasome by pyrogenic silica, but not by Stöber silica; activation was via a non-lysosomal process; few pyrogenic particles were taken up into the cells but collected on the surface membrane; in contrast, most Stöber silica particles were internalised	Zhang et al. (2012)
BALB/C mouse monocyte macrophage J774 cell line	Ludox SM sol*	2–335 nm	Commercial	Cytotoxicity (WST-1), 24 h; aggregationDMEM without serum	IC50 6–9 mg/L toxicity is dependent of surface area, but not state of aggregation (does not change surface area)	Rabolli et al. 2011)
MH-S mouse alveolar macrophages	Pyrogenic SAS (AEROSIL 300); precipitated SAS (FK 320)	298, 176 m2/g	Degussa	0, 5, 10, 20, 40, 80, 100, 150 µg/cm2, 24 h, LDH, ROS	Dose-dependent cytotoxicity ≥10 µg/cm2 (pyrogenic), ≥40 µg/cm2 (precipitated); ROS ≥ 20 µg/cm2 (pyrogenic), ≥ 40 µg/cm2 (precipitated)	Gazzano et al. (2012)
MH-S	NM-200, NM-203	189, 203 m2/g	JRC	2.5, 5, 10, 20, 40, 80 µg/cm2;24,48, 72 h	≥5 µg/m2: ↑inflammatory cytokines (NM-200 less than NM-203)	Di Cristo et al. (2016)
MH-S	NM-200, NM-203	14 nm (NM-200), 13 nm (NM-203)/189.2 m2/g and 203.9 m2/g	JRC	1–100 mg/L, 24, 48, 72 h, viability (LDH),	NM-200: IC50 (72 h) 25–60 mg/L;NM-203: IC50(72 h) < 10 mg/L	Farcal et al. (2015)
Fibroblasts
Mouse 3T3 fibroblasts	Ludox TM50*	38 nm (hydrodynamic diameter, DLS)	Commercial	5–100 mg/L for 24 h, XTT viability assay	Viability decreased to about 60 % at 100 mg/L; in DMEM with FCS increase in particle size due to agglomeration and reduced toxicity	Drescher et al. (2011)
Mouse 3T3 fibroblasts	Ludox SM sol*	2–335 nm, 283–331 m2/g	Commercial	Cytotoxicity (WST-1), 24 h; aggregation; DMEM without serum	IC50 15–22 mg/L; toxicity is dependent of surface area	Rabolli et al. (2011)
Mouse 3T3 fibroblasts	Food-grade SAS	14 nm/191 m2/g	Commercial	0–15 mg/L (6 day); 0–30 mg/L (3 day); [silica was used as negative control]	No effects on MTT conversion and DNA content	Brunner et al. (2006)
Mouse embryonic fibroblast (NIH/3T3)	Stöber silica*	20, 40, 60, 80, 100, 150, 200 nm	Laboratory	10–500 mg/L. 24, 72 h, viability, MTT, LDH	≥100 mg/L: some cytotoxicity; at 500 mg/L, 60 nm particles caused an extreme reduction in viability	Kim et al. (2015)
Human skin and lung fibroblasts (WS1; CCD-966sk; MRC-5 s); Human A549 lung epithelial tumour	Precipitated from sodium silicate or TEOS*	21, 80 (TEOS) nm; hydrodynamic size 188.3 and 236.3 (TEOS)	Laboratory	Up to 667 mg/L, 48 h, MTT, LDH	Slightly cytotoxic at high concentrations (≥138 mg/L); fibroblast cells with long doubling times more susceptible than tumour cells with short doubling times	Chang et al. (2007)
Nerve system
SH-SY5Y neuronal cell line	LUDOX® AS-20*, CL* and AM, polygon*	16.9, 13.3, 15.3 nm; charge (pH, stabiliser): neg (9.1, NH4OH), pos (4.5, NaCl alumina coated, neg (8.9, sodium aluminate)	Commercial	48 h, up to 1000 mg/L	Cell viability↓ at ≥100 mg/L (AS-20, AM) or >1000 mg/L (CL; MTT assay); intracellular ROS↑ at >100 mg/L only in AS-20 and AM-treated cells; Comet assay inconclusive	Kim et al. (2010)
Kidney
Human embryonic kidney (HEK293) cells	Colloidal silica*	20, 50 nm	Laboratory	20–100 mg/L, 24 h; cell viability, mitochondrial function, cell morphology, ROS, GSH, thiobarbituric acid reactive substance (TBARS), cell cycle, apoptosis	IC50 (viability): 80–140 mg/L; ↑ROS ↓GSH, lipid peroxidation, G2/M phase arrest, and ↑apoptotic sub-G1 population	Wang et al. (2009)
Testicular cells
TM3 Leydig and TM4 Sertoli cells	NM-200, NM-203	14 nm (NM-200), 13 nm (NM-203)/189.2 m2/g and 203.9 m2/g	JRC	0.125–200 mg/L, 24, 48, 72 h, viability (WST-1),	IC50 ≥ 100 mg/L in all cases	Farcal et al. (2015)

d Day, DLS dynamic light scattering, DMEM/F-12 Dulbecco’s modified eagle medium/nutrient mixture F-12, FCS foetal calf serum, GSH glutathione, IC 50 concentration causing 50 % inhibition, LDH lactate dehydrogenase, MEM minimum essential medium, na not available, nr not reported, MTS cytotoxicity test based on the reduction in MTS tetrazolium compound by viable cell, MTT 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide test, ROS reactive oxygen species, RT room temperature, SSA specific surface area

* Substance which does not fulfil the current EU criteria for E 551 (no star does, however, not necessarily implicate that the substance would be in compliance with EU E 551 specifications)

Discussion

The smallest constituent particles in E 551 are the aggregates, in which no primary particles are discernible. In order to exert its technological function as anti-caking agent in foods, E 551 must be present in sizes greater than 100 nm, and nanosized particles are therefore actually not desired. The results of aggregate size measurements are much influenced by sample preparation, including dispersion and dilution methods, and show volume weighted external diameters generally greater than 100 nm when using several different particle sizing techniques (data on file). This is in agreement with analytical results from the JRC showing E 551 aggregate sizes of greater than 100 nm when measured by dynamic light scattering (DLS) after applying ultrasonic dispersion at around 3000 Joule and filtering off larger particles (Barahona et al. 2016). Number weighted particle size distributions are currently not measurable with standardised methods, and estimates suffer from the assumption that SAS aggregates are spherical particles, which is inaccurate in the case of E 551. From the results of a recent study within the EU FP7-funded NanoDefine project (http://www.nanodefine.eu/) using several available particle sizing techniques, it has been concluded that “…the determination of the smallest external dimension remains challenging (if possible at all) for several classes of morphology, e.g., for three-dimensional aggregates…” (Babick et al. 2016). In this study, a stabilised suspension of fumed silica particles (colloidal silica) was used, which must not to be confused with E 551.

After oral intake, it might be possible that E 551 disintegrates during its passage through the intestinal tract. Available evidence, however, shows that there is no release of small particles in the acidic environment of the stomach. In more distal parts of the intestines, the more alkaline pH environment may cause some dissolution and formation of orthosilicic acid. As shown by van der Zande (van der Zande et al. 2014), silicon dioxide particles in the nanosize range (defined by the study authors to be below 200 nm) are found in the colon of non-E 551-exposed animals. The finding is also in agreement with biodurability studies performed in Caco-2 medium by the JRC (JRC 2013).

The overall evidence also shows that there is no indication that oral intake of E 551 may lead to liver toxicity. Studies cited in literature that show liver toxicity after amorphous silica exposure either relate to dye-labelled material, to biogenic material, or to exposures by the intravenous route. Studies with dye-labelled material cannot be read across to E 551, since the tested materials are different from E 551, and the influence of the dye on stability, distribution, and toxicity are not sufficiently known. Liver fibrosis could be induced after intravenous injection of amorphous silica particles (though colloidal, and not E 551) at doses of 50 mg/kg bw or even higher. Such exposures have been shown to activate Kupffer cells with an increase in inflammatory markers and subtle biochemical changes, including a slight increase in AST in mice, minor to moderate histopathological changes, and inflammatory liver granulomas in rats (Chen et al. 2013; Downs et al. 2012). Liver granulomas containing resident (Kupffer cells) and recruited macrophages and lymphocytes were also found after intravenous injection of pyrogenic SAS at 7 mg/kg, but there were no changes in biochemical serum marker. This finding was attributed to particle clearance mechanisms including uptake by macrophages and lysosomal degradation (Ivanov et al. 2012). However, systemic exposure levels, and hence also liver tissue exposure levels, that can be achieved by intravenous bolus injection cannot be reached after oral intake or gavage. There is therefore no basis for the assumption of a macrophage-induced liver re-modelling and fibrosis after oral exposure, a pathway that theoretically might be possible, see, for example, (Ju and Tacke 2016).

In none of the several other available studies, including an ongoing 90-day study with pyrogenic SAS in the low-dose range (NANoREG 2015), was any sign of liver fibrosis seen, nor any biochemical changes relating to liver toxicity found. To derive effect levels or calculating benchmark doses for E 551 based on the reported liver fibrosis in a single study (Jacobs et al. 2015) is therefore not justified.

In none of the available in vivo studies was there any adverse local effect on the gastrointestinal tract reported. Small but significant increase in villus heights and crypt depths in the jejunum, but no significant differences in the ratio between the villus height and crypt depth were found by van der Zande et al. (van der Zande et al. 2014). According to the study authors, long villi and a high villus:crypt ratio indicate a highly differentiated and active tissue. Gene set enrichment analysis on microarray data of jejunal epithelial samples from either the 28 or 84 days of exposure to both SAS and NM-202 did not show differences in gene expression profiles between the treatment groups and the controls. This finding is therefore not considered to be adverse.

It has previously been hypothesised that accumulation of non-degradable particles in human Peyer’s patch macrophages could exacerbate inflammation in Crohn’s disease (CD), but this could not be confirmed in human studies (Lomer et al. 2004). In work published in the form of a doctoral thesis, a silica-enriched diet containing 0.1 % SAS (which was not a product with the specifications of E 551) exacerbated the symptoms of irritable bowel disease (IBD) and enhanced mucosal inflammation in murine models of colitis (HA-VILLIN, DSS-induced acute and chronic colitis). SAS had no effects in healthy mice used as controls. Oxidative DNA damage was even decreased in the colon tissue of colitis-induced mice and of healthy mice treated with the SAS-enriched diet when compared to controls (Winter 2010). An upregulation of MHC-II, CD80, and CD86 on dendritic cells (DC) was found in vitro, and pro-inflammatory IL-beta was increased in endotoxin-activated DCs (Winter et al. 2011). In view of the absence in animal studies of any effects indicating immunotoxicity, and given that silicon dioxide particles are amongst the most abundant substances in the environment, including vegetable foods and the fact that the mucosa of the gastrointestinal tract is therefore continuously exposed, it is unlikely that gut-associated lymphoid tissue and immunity could be adversely affected by the presence of silicon dioxide as used for E 551.

In vitro, neither pyrogenic nor precipitated silica affected the cell barrier integrity in Caco-2 intestinal cells, even after long-term exposure (7–21 days); with both pyrogenic and precipitated silica cytotoxic and cytostatic effects were registered only at very high concentrations (≥100 mg/L in human gastric epithelial cells (GES-1) and Caco-2 cells) (Contado et al. 2016; Farcal et al. 2015; Yang et al. 2014). In liver cells, GSH depletion and oxidative stress have been found only at very high test concentrations (≥200 mg/L), or long incubation times (25 mg/L for 72 h); aspartate aminotransferase in buffalo rat liver cells incubated with supernatant from SiO2-stimulated Kupffer cells (resident hepatic macrophages) was only increased at ≥400 mg SiO2/L (Chen et al. 2013). Overall, these results do not demonstrate a particular sensitivity of the gastrointestinal tract or the liver towards silica particles. A reported “brush border disruption” in Caco-2 cells (Yang et al. 2016) after treatment with food-grade silica at concentrations of 0.01, 0.1, and 1 mg/L for 24 h is not considered to be of relevance for the in vivo situation where the mucus layer is the major barrier for absorption (Fröhlich and Roblegg 2012). Furthermore, no adverse local effects on the gastrointestinal mucosa have been found in any of the animal studies. In some in vitro experiments with murine macrophages, pyrogenic silica showed effects at lower concentrations than precipitated silica (Di Cristo et al. 2016; Gazzano et al. 2012; Zhang et al. 2012), while there were no significant differences in other cell types, including Caco-2 intestinal or testicular cells (Farcal et al. 2012). The difference tentatively has been ascribed to the complete coverage of precipitated silica with water which would protect the cells from any effect caused by direct contact between particle surface and cell membrane. From further experiments it appears that also a complete dehydroxylation would reduce silica surface reactivity. Any intermediate situation would be more reactive due to the complex interplay between cell membrane and particle surface (Gazzano et al. 2012). Zhang et al. (2012) see the main factor in an energetic unstable condition with radical formation on the surface of pyrogenic silica. [It is noted that Gazzano et al. did not find evidence of radical formation, neither on the surface of pyrogenic nor that of precipitated silica using electron paramagnetic resonance (EPR)/electron spin resonance (ESR), a suitable method for silica nanomaterials according to an OECD review (OECD 2016)]. Information from in vitro studies with regard to cellular uptake in macrophages is contradictory: in one study it is reported that pyrogenic silica is more efficiently taken up when compared with precipitated silica (Di Cristo et al. 2016); in another study this was not the case (Zhang et al. 2012). Others hypothesise that differences in agglomeration state and solubility are responsible for different biological effects in vitro (Aureli et al. 2012), but there is also evidence to the contrary (Di Cristo et al. 2016; Rabolli et al. 2011). Slight increases in pro-inflammatory cytokines (but no overt toxicity) were observed at ≥5 µg/cm2 in murine macrophages (cf Table 6) . Apart from the fact that the relevance of this in vitro murine model is questionable with regard to humans, see, for example, (Erbel et al. 2013; Mestas and Hughes 2004), the exposure levels were unrealistically high. Even under the assumption of direct contact with the intestinal epithelium (which in fact is protected by a mucous layer and is composed of different cell types) and an intake of up to 1500 mg/day (EFSA 2009), the amount of E 551 in the intestinal lumen would not reach an in vitro effective dose level (taking into account that the mean total mucosal surface of the digestive tract interior averages 32 m2 (Helander and Fandriks 2014), up to 4.6 µg/cm2 could theoretically be estimated for the intestinal lumen). The described differences in vitro are therefore not relevant for the in vivo situation after oral exposure to E 551. This is supported by the lack of effects in oral animal studies.

Conclusion

The systemic availability of E 551 is very low. There is no evidence for the release of nanosized particles in the stomach, but some dissolution and formation of orthosilicic acid may occur in the lower intestinal tract. None of the tested E 551 products caused any adverse local or systemic effect in oral repeated-dose, fertility, and developmental toxicity studies. In particular, there were no signs indicative of liver toxicity or macrophage-induced liver re-modelling even at very high oral doses. There were also no indications of immunotoxicity or neurotoxicity. Reported effects after low-dose exposure to pyrogenic SAS are all within the normal physiological range and cannot be considered as adverse. SAS was not mutagenic or genotoxic in vivo. In intact cells, a direct interaction of unlabelled and unmodified SAS with DNA was never found. Differences in the magnitude of biological responses between pyrogenic and precipitated silica described in some in vitro studies with murine macrophages at exaggerated exposures seem to be related to interactions with cell culture proteins and cell membranes. The available toxicological evidence in vivo shows that there is no difference in the toxicity of E 551 products, independent of their manufacturing method. Based on the available evidence, it is concluded that silicon dioxide used as a food additive (E 551) is a substance of very low toxicity which based on the total dietary intake (from its use as a food additive, and its use in dietary supplements) does not represent a human health risk. Any new or novel forms of silicon dioxide that do not comply with established specifications, or are produced to perform a new technological function in food, would require specific safety and risk assessments.