Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Autoregulation of retinal homeobox (rax) gene promoter activity through a highly conserved genomic element.

Literature DB >> 27696680

Autoregulation of retinal homeobox (rax) gene promoter activity through a highly conserved genomic element.

Lisa E Kelly¹, Reyna I Martinez-De Luna², Heithem M El-Hodiri^1,2,3.

Abstract

The Retinal homeobox (rax) gene is expressed in vertebrate retinal progenitor and stem cells and is essential for retinal development. In frogs, rax is expressed in the ciliary marginal zone (CMZ), a region containing retinal progenitor and stem cells at the anterior of the eye. Little is known regarding regulation of rax transcription and regulation of transcription of rax targets. We found that three ultra-conserved genomic elements (UCEs) flanking the rax coding region regulate expression of a rax promoter-GFP transgene in Xenopus tadpoles. One of these elements, UCE1, regulates expression of the transgene in the dorsal CMZ. UCE1 contains a Rax binding site, PCE-1. We demonstrate that rax regulates expression of the transgene through the PCE-1 site found in UCE1. Therefore, rax transcription in the CMZ is controlled, in part, by autoregulatory mechanisms.

Entities: Chemical Disease Gene Species

Keywords: Xenopus; ciliary marginal zone; genomic element; retinal homeobox gene; retinal progenitor cells

Mesh：

Substances：

Year: 2016 PMID： 27696680 PMCID： PMC5118134 DOI： 10.1002/dvg.22983

Source DB: PubMed Journal: Genesis ISSN： 1526-954X Impact factor: 2.487

31 in total

1. A simplified method of generating transgenic Xenopus.

Authors: D B Sparrow; B Latinkic; T J Mohun
Journal: Nucleic Acids Res Date: 2000-02-15 Impact factor: 16.971

2. Asymmetric growth and development of the Xenopus laevis retina during metamorphosis is controlled by type III deiodinase.

Authors: N Marsh-Armstrong; H Huang; B F Remo; T T Liu; D D Brown
Journal: Neuron Date: 1999-12 Impact factor: 17.173

3. Xenopus rhodopsin promoter. Identification of immediate upstream sequences necessary for high level, rod-specific transcription.

Authors: S S Mani; S Batni; L Whitaker; S Chen; G Engbretson; B E Knox
Journal: J Biol Chem Date: 2001-05-01 Impact factor: 5.157

4. Characterization of the Xenopus rhodopsin gene.

Authors: S Batni; L Scalzetti; S A Moody; B E Knox
Journal: J Biol Chem Date: 1996-02-09 Impact factor: 5.157

5. Five Nkx5 genes show differential expression patterns in anlagen of sensory organs in medaka: insight into the evolution of the gene family.

Authors: M Adamska; A Wolff; M Kreusler; J Wittbrodt; T Braun; E Bober
Journal: Dev Genes Evol Date: 2001-07 Impact factor: 0.900

6. The proximal promoter of the mouse arrestin gene directs gene expression in photoreceptor cells and contains an evolutionarily conserved retinal factor-binding site.

Authors: T Kikuchi; K Raju; M L Breitman; T Shinohara
Journal: Mol Cell Biol Date: 1993-07 Impact factor: 4.272

Autoregulation of retinal homeobox (rax) gene promoter activity through a highly conserved genomic element.

1. A simplified method of generating transgenic Xenopus.

2. Asymmetric growth and development of the Xenopus laevis retina during metamorphosis is controlled by type III deiodinase.

3. Xenopus rhodopsin promoter. Identification of immediate upstream sequences necessary for high level, rod-specific transcription.

4. Characterization of the Xenopus rhodopsin gene.

5. Five Nkx5 genes show differential expression patterns in anlagen of sensory organs in medaka: insight into the evolution of the gene family.

6. The proximal promoter of the mouse arrestin gene directs gene expression in photoreceptor cells and contains an evolutionarily conserved retinal factor-binding site.

7. The Rx-like homeobox gene (Rx-L) is necessary for normal photoreceptor development.

8. Xrx1, a novel Xenopus homeobox gene expressed during eye and pineal gland development.

9. Regulation of photoreceptor gene expression by the retinal homeobox (Rx) gene product.

10. Wnt-1-dependent regulation of local E-cadherin and alpha N-catenin expression in the embryonic mouse brain.

1. Impact of different promoters, promoter mutation, and an enhancer on recombinant protein expression in CHO cells.

2. The rax homeobox gene is mutated in the eyeless axolotl, Ambystoma mexicanum.

3. The CAG promoter maintains high-level transgene expression in HEK293 cells.