Literature DB >> 10648800

A simplified method of generating transgenic Xenopus.

D B Sparrow1, B Latinkic, T J Mohun.   

Abstract

Currently transgenic frog embryos are generated using restriction-enzyme-mediated integration (REMI) on decondensed sperm nuclei followed by nuclear transplantation into unfertilized eggs. We have developed a simplified version of this protocol that has the potential to increase the numbers of normally developing transgenic embryos.

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Year:  2000        PMID： 10648800      PMCID： PMC102591          DOI： 10.1093/nar/28.4.e12

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  6 in total

1.  Mammalian transgenesis by intracytoplasmic sperm injection.

Authors:  A C Perry; T Wakayama; H Kishikawa; T Kasai; M Okabe; Y Toyoda; R Yanagimachi
Journal:  Science       Date:  1999-05-14       Impact factor: 47.728

2.  Persistence and replication of plasmid DNA microinjected into early embryos of Xenopus laevis.

Authors:  N J Marini; L D Etkin; R M Benbow
Journal:  Dev Biol       Date:  1988-06       Impact factor: 3.582

3.  Interactions between Xwnt-8 and Spemann organizer signaling pathways generate dorsoventral pattern in the embryonic mesoderm of Xenopus.

Authors:  J L Christian; R T Moon
Journal:  Genes Dev       Date:  1993-01       Impact factor: 11.361

4.  Metamorphosis is inhibited in transgenic Xenopus laevis tadpoles that overexpress type III deiodinase.

Authors:  H Huang; N Marsh-Armstrong; D D Brown
Journal:  Proc Natl Acad Sci U S A       Date:  1999-02-02       Impact factor: 11.205

5.  Transgenic Xenopus embryos from sperm nuclear transplantations reveal FGF signaling requirements during gastrulation.

Authors:  K L Kroll; E Amaya
Journal:  Development       Date:  1996-10       Impact factor: 6.868

6.  Upstream sequences required for tissue-specific activation of the cardiac actin gene in Xenopus laevis embryos.

Authors:  T J Mohun; N Garrett; J B Gurdon
Journal:  EMBO J       Date:  1986-12-01       Impact factor: 11.598
  6 in total
  43 in total

1.  FLP and Cre recombinase function in Xenopus embryos.

Authors:  D Werdien; G Peiler; G U Ryffel
Journal:  Nucleic Acids Res       Date:  2001-06-01       Impact factor: 16.971

2.  Tagging muscle cell lineages in development and tail regeneration using Cre recombinase in transgenic Xenopus.

Authors:  Gerhart U Ryffel; Dagmar Werdien; Gülüzar Turan; Andrea Gerhards; Stefan Goosses; Sabine Senkel
Journal:  Nucleic Acids Res       Date:  2003-04-15       Impact factor: 16.971

3.  A cell-specific transgenic approach in Xenopus reveals the importance of a functional p24 system for a secretory cell.

Authors:  Gerrit Bouw; Rick Van Huizen; Eric J R Jansen; Gerard J M Martens
Journal:  Mol Biol Cell       Date:  2003-12-29       Impact factor: 4.138

4.  A functional survey of the enhancer activity of conserved non-coding sequences from vertebrate Iroquois cluster gene deserts.

Authors:  Elisa de la Calle-Mustienes; Cármen Gloria Feijóo; Miguel Manzanares; Juan J Tena; Elisa Rodríguez-Seguel; Annalisa Letizia; Miguel L Allende; José Luis Gómez-Skarmeta
Journal:  Genome Res       Date:  2005-07-15       Impact factor: 9.043

5.  Transgenic Xenopus laevis embryos can be generated using phiC31 integrase.

Authors:  Bryan G Allen; Daniel L Weeks
Journal:  Nat Methods       Date:  2005-12       Impact factor: 28.547

6.  Organization of cGMP sensing structures on the rod photoreceptor outer segment plasma membrane.

Authors:  Ina Nemet; Guilian Tian; Yoshikazu Imanishi
Journal:  Channels (Austin)       Date:  2014       Impact factor: 2.581

7.  Autoregulation of retinal homeobox (rax) gene promoter activity through a highly conserved genomic element.

Authors:  Lisa E Kelly; Reyna I Martinez-De Luna; Heithem M El-Hodiri
Journal:  Genesis       Date:  2016-10-19       Impact factor: 2.487

8.  Axial Skeletal Malformations in Genetically Modified Xenopus laevis and Xenopus tropicalis.

Authors:  Anne L Zlatow; Sabrina S Wilson; Donna M Bouley; Joanne Tetens-Woodring; Daniel R Buchholz; Sherril L Green
Journal:  Comp Med       Date:  2020-11-17       Impact factor: 0.982

9.  Manipulating heat shock factor-1 in Xenopus tadpoles: neuronal tissues are refractory to exogenous expression.

Authors:  Ron P Dirks; Remon van Geel; Sanne M M Hensen; Siebe T van Genesen; Nicolette H Lubsen
Journal:  PLoS One       Date:  2010-04-13       Impact factor: 3.240

10.  Regulation of protocadherin gene expression by multiple neuron-restrictive silencer elements scattered in the gene cluster.

Authors:  Yuen-Peng Tan; Shaobing Li; Xiao-Juan Jiang; Wailin Loh; Yik Khon Foo; Chay-Boon Loh; Qiurong Xu; Wai-Hong Yuen; Michael Jones; Jianlin Fu; Byrappa Venkatesh; Wei-Ping Yu
Journal:  Nucleic Acids Res       Date:  2010-04-12       Impact factor: 16.971
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