Cody T Haun1, Wesley C Kephart1, Angelia M Holland1, Christopher B Mobley1, Anna E McCloskey1, Joshua J Shake1, David D Pascoe1, Michael D Roberts1,2, Jeffrey S Martin3,4. 1. School of Kinesiology, Auburn University, Auburn, AL, 36849, USA. 2. Department of Cell Biology and Physiology, Edward Via College of Osteopathic Medicine-Auburn Campus, 910 S. Donahue Drive, Auburn, AL, 36832, USA. 3. School of Kinesiology, Auburn University, Auburn, AL, 36849, USA. jmartin@auburn.vcom.edu. 4. Department of Cell Biology and Physiology, Edward Via College of Osteopathic Medicine-Auburn Campus, 910 S. Donahue Drive, Auburn, AL, 36832, USA. jmartin@auburn.vcom.edu.
Abstract
INTRODUCTION:Inorganic nitrate ingestion has been posited to affect arterial blood pressure and vascular function. PURPOSE: We sought to determine the acute effect of a red spinach extract (RSE) high in inorganic nitrate on vascular reactivity 1-h after ingestion in peripheral conduit and resistance arteries. METHODS: Fifteen (n = 15; males 8, females 7) apparently healthy subjects (aged 23.1 ± 3.3 years; BMI 27.2 ± 3.7 kg/m2) participated in this crossover design, double-blinded study. Subjects reported to the lab ≥2-h post-prandial and consumed RSE (1000 mg dose; ~90 mg nitrate) or placebo (PBO). Venipuncture was performed on three occasions: baseline, 30-min post-ingestion and between 65 to 75-min post-ingestion. Baseline vascular measurements [i.e., calf venous occlusion plethysmography, brachial artery flow-mediated dilation (FMD)], 30-min of continuous blood pressure (BP) and heart rate (HR) analysis, and follow-up vascular measurements beginning at 40-min post-ingestion were also performed. RESULTS:Humoral nitrate following RSE ingestion was significantly higher at 30- (+54 %; P = 0.039) and 65 to 75-min post-ingestion compared to baseline (+255 %, P < 0.001) and PBO at the same time points (P < 0.05). No significant changes in BP or HR occurred in either condition. Peak reactive hyperemia (RH) calf blood flow increased significantly (+13.7 %; P = 0.016) following RSE ingestion, whereas it decreased (-14.0 %; P = 0.008) following PBO ingestion. No significant differential FMD responses were detected (P > 0.05), though RH was decreased following the baseline measure in both conditions. CONCLUSIONS:RSE significantly increased plasma nitrate 30-min post-ingestion, but acute microvascular (i.e., resistance vasculature) reactivity increases were isolated to the lower limb and no appreciable change in brachial artery FMD was observed.
RCT Entities:
INTRODUCTION:Inorganic nitrate ingestion has been posited to affect arterial blood pressure and vascular function. PURPOSE: We sought to determine the acute effect of a red spinach extract (RSE) high in inorganic nitrate on vascular reactivity 1-h after ingestion in peripheral conduit and resistance arteries. METHODS: Fifteen (n = 15; males 8, females 7) apparently healthy subjects (aged 23.1 ± 3.3 years; BMI 27.2 ± 3.7 kg/m2) participated in this crossover design, double-blinded study. Subjects reported to the lab ≥2-h post-prandial and consumed RSE (1000 mg dose; ~90 mg nitrate) or placebo (PBO). Venipuncture was performed on three occasions: baseline, 30-min post-ingestion and between 65 to 75-min post-ingestion. Baseline vascular measurements [i.e., calf venous occlusion plethysmography, brachial artery flow-mediated dilation (FMD)], 30-min of continuous blood pressure (BP) and heart rate (HR) analysis, and follow-up vascular measurements beginning at 40-min post-ingestion were also performed. RESULTS: Humoral nitrate following RSE ingestion was significantly higher at 30- (+54 %; P = 0.039) and 65 to 75-min post-ingestion compared to baseline (+255 %, P < 0.001) and PBO at the same time points (P < 0.05). No significant changes in BP or HR occurred in either condition. Peak reactive hyperemia (RH) calf blood flow increased significantly (+13.7 %; P = 0.016) following RSE ingestion, whereas it decreased (-14.0 %; P = 0.008) following PBO ingestion. No significant differential FMD responses were detected (P > 0.05), though RH was decreased following the baseline measure in both conditions. CONCLUSIONS:RSE significantly increased plasma nitrate 30-min post-ingestion, but acute microvascular (i.e., resistance vasculature) reactivity increases were isolated to the lower limb and no appreciable change in brachial artery FMD was observed.
Authors: Clément Gautier; Ernst van Faassen; Ivan Mikula; Pavel Martasek; Anny Slama-Schwok Journal: Biochem Biophys Res Commun Date: 2006-01-19 Impact factor: 3.575
Authors: James E Sharman; Richard Lim; Ahmad M Qasem; Jeff S Coombes; Malcolm I Burgess; Jeff Franco; Paul Garrahy; Ian B Wilkinson; Thomas H Marwick Journal: Hypertension Date: 2006-05-01 Impact factor: 10.190
Authors: Andrew M Jones; Scott K Ferguson; Stephen J Bailey; Anni Vanhatalo; David C Poole Journal: Exerc Sport Sci Rev Date: 2016-04 Impact factor: 6.230
Authors: Petey W Mumford; Wesley C Kephart; Matthew A Romero; Cody T Haun; C Brooks Mobley; Shelby C Osburn; James C Healy; Angelique N Moore; David D Pascoe; William C Ruffin; Darren T Beck; Jeffrey S Martin; Michael D Roberts; Kaelin C Young Journal: Eur J Appl Physiol Date: 2018-08-28 Impact factor: 3.078
Authors: Angelique N Moore; Cody T Haun; Wesley C Kephart; Angelia M Holland; Christopher B Mobley; David D Pascoe; Michael D Roberts; Jeffrey S Martin Journal: Sports (Basel) Date: 2017-10-16
Authors: Jeffrey S Martin; Petey W Mumford; Cody T Haun; Micheal J Luera; Tyler W D Muddle; Ryan J Colquhoun; Mary P Feeney; Cameron S Mackey; Paul A Roberson; Kaelin C Young; David D Pascoe; Jason M DeFreitas; Nathaniel D M Jenkins; Michael D Roberts Journal: J Int Soc Sports Nutr Date: 2017-09-26 Impact factor: 5.150
Authors: James T Haynes; Jeremy R Townsend; Marko A Aziz; Megan D Jones; Laurel A Littlefield; Matthew D Ruiz; Kent D Johnson; Adam M Gonzalez Journal: Sports (Basel) Date: 2021-05-27
Authors: Carlton D Fox; Christian T Garner; Petey W Mumford; Darren T Beck; Michael D Roberts Journal: J Int Soc Sports Nutr Date: 2020-05-27 Impact factor: 5.150