Jayne Moquet1, Stephen Barnard1, Albena Staynova2, Carita Lindholm3, Octávia Monteiro Gil4, Vanda Martins4, Ute Rößler5, Anne Vral6, Charlot Vandevoorde6,7, Maria Wojewódzka8, Kai Rothkamm1,9. 1. a Public Health England, Centre for Radiation Chemical and Environmental Hazards , Chilton , UK. 2. b National Centre of Radiobiology and Radiation Protection , Sofia , Bulgaria. 3. c Radiation and Nuclear Safety Authority (STUK) , Helsinki , Finland. 4. d Instituto Superior Técnico, Universidade de Lisboa, C2TN, Bobadela-LRS , Portugal. 5. e Bundesamt für Strahlenschutz , Oberschleissheim , Germany. 6. f Department of Basic Medical Sciences , Ghent University , Ghent , Belgium. 7. g Themba LABS, National Research Foundation , Somerset West , South Africa. 8. h Institute of Nuclear Chemistry and Technology, Centre for Radiobiology and Biological Dosimetry , Warsaw , Poland. 9. i Department of Radiotherapy & Radio-Oncology , University Medical Centre Hamburg-Eppendorf , Hamburg , Germany.
Abstract
PURPOSE: Within the EU RENEB project, seven laboratories have taken part in training and harmonisation activities to strengthen triage gamma-H2AX-based radiation exposure assessment. This has culminated in a second triage biodosimetry exercise. MATERIALS AND METHODS: Whole blood and separated lymphocyte samples were homogenously irradiated with 60Co gamma rays at 0.5, 2.5 (blind samples), 0 and 2 Gy (reference samples). Following post-exposure incubations of 4 and 24 h, 16 samples were shipped on ice packs to each partner. The samples were stained and scored for gamma-H2AX foci, using manual and/or automated fluorescence microscope scoring strategies. Dose estimates were obtained and used to assign triage categories to the samples. RESULTS: Average dose estimates across all the laboratories correlated well with true doses. The most accurate assignment of triage category was achieved by manual scoring of the 4-h blood and lymphocyte samples. Only three samples out of a total of 46 were miscategorized in a way that could have adversely effected the clinical management of a radiation casualty. CONCLUSIONS: This inter-comparison exercise has demonstrated that following a recent acute radiation exposure, the gamma-H2AX assay could be a useful triage tool that can be successfully applied across a network of laboratories.
PURPOSE: Within the EU RENEB project, seven laboratories have taken part in training and harmonisation activities to strengthen triage gamma-H2AX-based radiation exposure assessment. This has culminated in a second triage biodosimetry exercise. MATERIALS AND METHODS: Whole blood and separated lymphocyte samples were homogenously irradiated with 60Co gamma rays at 0.5, 2.5 (blind samples), 0 and 2 Gy (reference samples). Following post-exposure incubations of 4 and 24 h, 16 samples were shipped on ice packs to each partner. The samples were stained and scored for gamma-H2AX foci, using manual and/or automated fluorescence microscope scoring strategies. Dose estimates were obtained and used to assign triage categories to the samples. RESULTS: Average dose estimates across all the laboratories correlated well with true doses. The most accurate assignment of triage category was achieved by manual scoring of the 4-h blood and lymphocyte samples. Only three samples out of a total of 46 were miscategorized in a way that could have adversely effected the clinical management of a radiation casualty. CONCLUSIONS: This inter-comparison exercise has demonstrated that following a recent acute radiation exposure, the gamma-H2AX assay could be a useful triage tool that can be successfully applied across a network of laboratories.
Authors: Richard Harbron; Elizabeth A Ainsbury; Simon D Bouffler; Rick J Tanner; Jonathan S Eakins; Mark S Pearce Journal: Br J Radiol Date: 2017-08-22 Impact factor: 3.039
Authors: Kevin Lee Min Chua; Eugenia Li Ling Yeo; Waseem Ahamed Shihabudeen; Sze Huey Tan; Than Than Shwe; Enya Hui Wen Ong; Paula Yeng Po Lam; Khee Chee Soo; Yoke Lim Soong; Kam Weng Fong; Terence Wee Kiat Tan; Joseph Tien Seng Wee; Melvin Lee Kiang Chua Journal: BMC Cancer Date: 2018-11-12 Impact factor: 4.430
Authors: Jochen Einbeck; Elizabeth A Ainsbury; Rachel Sales; Stephen Barnard; Felix Kaestle; Manuel Higueras Journal: PLoS One Date: 2018-11-28 Impact factor: 3.240