| Literature DB >> 27678250 |
Alberto Mezzetti1,2, Winfried Leibl3.
Abstract
Time-resolved (TR) infrared (IR) spectroscopy in the nanosecond to second timescale has been extensively used, in the last 30 years, in the study of photosynthetic systems. Interesting results have also been obtained at lower time resolution (minutes or even hours). In this review, we first describe the used techniques-dispersive IR, laser diode IR, rapid-scan Fourier transform (FT)IR, step-scan FTIR-underlying the advantages and disadvantages of each of them. Then, the main TR-IR results obtained so far in the investigation of photosynthetic reactions (in reaction centers, in light-harvesting systems, but also in entire membranes or even in living organisms) are presented. Finally, after the general conclusions, the perspectives in the field of TR-IR applied to photosynthesis are described.Keywords: Bacterial reaction centers; Electron transfer; FTIR difference spectroscopy; Infrared; Light-harvesting systems; Photosystem I; Photosystem II; Proton transfer; Rapid-scan FTIR; Reaction centers; Step-scan FTIR; Ubiquinone; Vibrational spectroscopy
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Year: 2016 PMID: 27678250 DOI: 10.1007/s11120-016-0305-3
Source DB: PubMed Journal: Photosynth Res ISSN: 0166-8595 Impact factor: 3.573