Divya Pankajakshan1, Maria T P Albuquerque2, Joshua D Evans1, Malgorzata M Kamocka3, Richard L Gregory1, Marco C Bottino4. 1. Department of Biomedical and Applied Sciences, Division of Dental Biomaterials, Indiana University School of Dentistry, Indianapolis, Indiana. 2. Department of Biomedical and Applied Sciences, Division of Dental Biomaterials, Indiana University School of Dentistry, Indianapolis, Indiana; Department of Clinical Dentistry, Endodontics, Federal University of Bahia, Salvador, Bahia, Brazil. 3. Division of Nephrology, Indiana University School of Dentistry, Indianapolis, Indiana. 4. Department of Biomedical and Applied Sciences, Division of Dental Biomaterials, Indiana University School of Dentistry, Indianapolis, Indiana. Electronic address: mbottino@iu.edu.
Abstract
INTRODUCTION: Root canal disinfection and the establishment of an intracanal microenvironment conducive to the proliferation/differentiation of stem cells play a significant role in regenerative endodontics. This study was designed to (1) investigate the antimicrobial efficacy of triple antibiotic-containing nanofibers against a dual-species biofilm and (2) evaluate the ability of dental pulp stem cells (DPSCs) to adhere to and proliferate on dentin upon nanofiber exposure. METHODS: Seven-day-old dual-species biofilm established on dentin specimens was exposed for 3 days to the following: saline (control), antibiotic-free nanofibers (control), and triple antibiotic-containing nanofibers or a saturated triple antibiotic paste (TAP) solution (50 mg/mL in phosphate buffer solution). Bacterial viability was assessed using the LIVE/DEAD assay (Molecular Probes, Inc, Eugene, OR) and confocal laser scanning microscopy. For cytocompatibility studies, dentin specimens after nanofiber or TAP (1 g/mL in phosphate buffer solution) exposure were evaluated for cell adhesion and spreading by actin-phalloidin staining. DPSC proliferation was assessed on days 1, 3, and 7. Statistics were performed, and significance was set at the 5% level. RESULTS: Confocal laser scanning microscopy showed significant bacterial death upon antibiotic-containing nanofiber exposure, differing significantly (P < .05) from antibiotic-free fibers and the control (saline). DPSCs showed enhanced adhesion/spreading on dentin specimens treated with antibiotic-containing nanofibers when compared with its TAP counterparts. The DPSC proliferation rate was similar on days 1 and 3 in antibiotic-free nanofibers, triple antibiotic-containing nanofibers, and TAP-treated dentin. Proliferation was higher (9-fold) on dentin treated with antibiotic-containing nanofibers on day 7 compared with TAP. CONCLUSIONS: Triple antibiotic-containing polymer nanofibers led to significant bacterial death, whereas they did not affect DPSC attachment and proliferation on dentin.
INTRODUCTION: Root canal disinfection and the establishment of an intracanal microenvironment conducive to the proliferation/differentiation of stem cells play a significant role in regenerative endodontics. This study was designed to (1) investigate the antimicrobial efficacy of triple antibiotic-containing nanofibers against a dual-species biofilm and (2) evaluate the ability of dental pulp stem cells (DPSCs) to adhere to and proliferate on dentin upon nanofiber exposure. METHODS: Seven-day-old dual-species biofilm established on dentin specimens was exposed for 3 days to the following: saline (control), antibiotic-free nanofibers (control), and triple antibiotic-containing nanofibers or a saturated triple antibiotic paste (TAP) solution (50 mg/mL in phosphate buffer solution). Bacterial viability was assessed using the LIVE/DEAD assay (Molecular Probes, Inc, Eugene, OR) and confocal laser scanning microscopy. For cytocompatibility studies, dentin specimens after nanofiber or TAP (1 g/mL in phosphate buffer solution) exposure were evaluated for cell adhesion and spreading by actin-phalloidin staining. DPSC proliferation was assessed on days 1, 3, and 7. Statistics were performed, and significance was set at the 5% level. RESULTS: Confocal laser scanning microscopy showed significant bacterial death upon antibiotic-containing nanofiber exposure, differing significantly (P < .05) from antibiotic-free fibers and the control (saline). DPSCs showed enhanced adhesion/spreading on dentin specimens treated with antibiotic-containing nanofibers when compared with its TAP counterparts. The DPSC proliferation rate was similar on days 1 and 3 in antibiotic-free nanofibers, triple antibiotic-containing nanofibers, and TAP-treated dentin. Proliferation was higher (9-fold) on dentin treated with antibiotic-containing nanofibers on day 7 compared with TAP. CONCLUSIONS: Triple antibiotic-containing polymer nanofibers led to significant bacterial death, whereas they did not affect DPSC attachment and proliferation on dentin.
Authors: Juliana Y Nagata; Adriana J Soares; Francisco J Souza-Filho; Alexandre A Zaia; Caio C R Ferraz; José F A Almeida; Brenda P F A Gomes Journal: J Endod Date: 2014-04-29 Impact factor: 4.171
Authors: Marco C Bottino; Ghaeth H Yassen; Jeffrey A Platt; Nawaf Labban; L Jack Windsor; Kenneth J Spolnik; Ana H A Bressiani Journal: J Tissue Eng Regen Med Date: 2013-03-08 Impact factor: 3.963
Authors: Margaret Louise A Porter; Eliseu A Münchow; Maria T P Albuquerque; Kenneth J Spolnik; Anderson T Hara; Marco C Bottino Journal: J Endod Date: 2015-10-23 Impact factor: 4.171
Authors: Marco C Bottino; Rodrigo A Arthur; R Aaron Waeiss; Krzysztof Kamocki; Karen S Gregson; Richard L Gregory Journal: Clin Oral Investig Date: 2014-02-18 Impact factor: 3.573
Authors: Misako Nakashima; Koichiro Iohara; Marco C Bottino; Ashraf F Fouad; Jacques E Nör; George T-J Huang Journal: Tissue Eng Part B Rev Date: 2019-01-09 Impact factor: 6.389
Authors: Agnes B Meireles; Daniella K Corrêa; João Vw da Silveira; Ana Lg Millás; Edison Bittencourt; Gustavo Ea de Brito-Melo; Libardo A González-Torres Journal: Exp Biol Med (Maywood) Date: 2018-05
Authors: Julian M Sotomil; Eliseu A Münchow; Divya Pankajakshan; Kenneth J Spolnik; Jessica A Ferreira; Richard L Gregory; Marco C Bottino Journal: J Endod Date: 2019-09-18 Impact factor: 4.171
Authors: Juliana S Ribeiro; Eliseu A Münchow; Ester A Ferreira Bordini; Wellington Luiz de Oliveira da Rosa; Marco C Bottino Journal: J Endod Date: 2020-09 Impact factor: 4.171
Authors: Marco C Bottino; Maria T P Albuquerque; Asma Azabi; Eliseu A Münchow; Kenneth J Spolnik; Jacques E Nör; Paul C Edwards Journal: J Biomed Mater Res B Appl Biomater Date: 2018-10-03 Impact factor: 3.368
Authors: Ashley Karczewski; Sabrina A Feitosa; Ethan I Hamer; Divya Pankajakshan; Richard L Gregory; Kenneth J Spolnik; Marco C Bottino Journal: J Endod Date: 2017-10-20 Impact factor: 4.171