Nardhy Gomez-Lopez1,2,3, Roberto Romero4,5,6,7, Marcia Arenas-Hernandez8,9,10, Hyunyoung Ahn8,9, Bogdan Panaitescu8,9, Felipe Vadillo-Ortega11, Carmen Sanchez-Torres10, Katherine S Salisbury9, Sonia S Hassan8,9. 1. Perinatology Research Branch, NICHD/NIH/DHHS, Bethesda, MD, and Detroit, MI, USA. nardhy.gomez-lopez@wayne.edu. 2. Department of Obstetrics & Gynecology, Wayne State University School of Medicine, Detroit, MI, USA. nardhy.gomez-lopez@wayne.edu. 3. Department of Immunology & Microbiology, Wayne State University School of Medicine, Detroit, MI, USA. nardhy.gomez-lopez@wayne.edu. 4. Perinatology Research Branch, NICHD/NIH/DHHS, Bethesda, MD, and Detroit, MI, USA. romeror@mail.nih.gov. 5. Department of Obstetrics & Gynecology, University of Michigan, Ann Arbor, MI, USA. romeror@mail.nih.gov. 6. Department of Epidemiology & Biostatistics, Michigan State University, East Lansing, MI, USA. romeror@mail.nih.gov. 7. Center for Molecular Obstetrics & Genetics, Wayne State University, Detroit, MI, USA. romeror@mail.nih.gov. 8. Perinatology Research Branch, NICHD/NIH/DHHS, Bethesda, MD, and Detroit, MI, USA. 9. Department of Obstetrics & Gynecology, Wayne State University School of Medicine, Detroit, MI, USA. 10. Department of Molecular Biomedicine, CINVESTAV, Mexico City, Mexico. 11. Unit of Vinculation, Faculty of Medicine, Universidad Nacional Autónoma de México en el Instituto Nacional de Medicina Genómica, Mexico City, Mexico.
Abstract
PROBLEM: Activated/effector T cells seem to play a role in the pathological inflammation associated with preterm labor. The aim of this study was to determine whether in vivo T-cell activation by a monoclonal αCD3ε antibody induces preterm labor and birth. METHOD OF STUDY: Pregnant B6 mice were intraperitoneally injected with a monoclonal αCD3ε antibody or its isotype control. The gestational age, the rates of preterm birth and pup mortality at birth as well as the fetal heart rate and umbilical artery pulsatility index were determined. RESULTS: Injection of a monoclonal αCD3ε antibody led to preterm labor/birth (αCD3ε 83 ± 16.97% [10/12] vs isotype 0% [0/8]) and increased the rate of pup mortality at birth (αCD3ε 87.30 ± 8.95% [77/85] vs isotype 4.91 ± 4.34% [3/59]). In addition, injection of a monoclonal αCD3ε antibody decreased the fetal heart rate and increased the umbilical artery pulsatility index when compared to the isotype control. CONCLUSION: In vivo T-cell activation by a monoclonal αCD3ε antibody in late gestation induces preterm labor and birth.
PROBLEM: Activated/effector T cells seem to play a role in the pathological inflammation associated with preterm labor. The aim of this study was to determine whether in vivo T-cell activation by a monoclonal αCD3ε antibody induces preterm labor and birth. METHOD OF STUDY: Pregnant B6 mice were intraperitoneally injected with a monoclonal αCD3ε antibody or its isotype control. The gestational age, the rates of preterm birth and pup mortality at birth as well as the fetal heart rate and umbilical artery pulsatility index were determined. RESULTS: Injection of a monoclonal αCD3ε antibody led to preterm labor/birth (αCD3ε 83 ± 16.97% [10/12] vs isotype 0% [0/8]) and increased the rate of pup mortality at birth (αCD3ε 87.30 ± 8.95% [77/85] vs isotype 4.91 ± 4.34% [3/59]). In addition, injection of a monoclonal αCD3ε antibody decreased the fetal heart rate and increased the umbilical artery pulsatility index when compared to the isotype control. CONCLUSION: In vivo T-cell activation by a monoclonal αCD3ε antibody in late gestation induces preterm labor and birth.
Authors: Uma Shanmugasundaram; J William Critchfield; Jane Pannell; Jean Perry; Linda C Giudice; Karen Smith-McCune; Ruth M Greenblatt; Barbara L Shacklett Journal: Am J Reprod Immunol Date: 2013-12-06 Impact factor: 3.886
Authors: D Levenson; R Romero; V Garcia-Flores; D Miller; Y Xu; A Sahi; S S Hassan; N Gomez-Lopez Journal: Clin Exp Immunol Date: 2020-05-07 Impact factor: 4.330
Authors: N Gomez-Lopez; R Romero; M Arenas-Hernandez; G Schwenkel; D St Louis; S S Hassan; T N Mial Journal: Clin Exp Immunol Date: 2017-05-10 Impact factor: 4.330
Authors: Pietro Presicce; Chan-Wook Park; Paranthaman Senthamaraikannan; Sandip Bhattacharyya; Courtney Jackson; Fansheng Kong; Cesar M Rueda; Emily DeFranco; Lisa A Miller; David A Hildeman; Nathan Salomonis; Claire A Chougnet; Alan H Jobe; Suhas G Kallapur Journal: JCI Insight Date: 2018-03-22