Yaozhu Leng1,2, Roberto Romero1,3,4,5, Yi Xu1,2, Jose Galaz1,6, Rebecca Slutsky1, Marcia Arenas-Hernandez1,2, Valeria Garcia-Flores1,2, Kenichiro Motomura1,2, Sonia S Hassan1,2,7, Andrea Reboldi8, Nardhy Gomez-Lopez1,2,9,10. 1. Perinatology Research Branch, Division of Obstetrics and Maternal-Fetal Medicine, Division of Intramural Research, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, U.S. Department of Health and Human Services, Bethesda, Maryland, and Detroit, Michigan. 2. Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, Michigan. 3. Department of Obstetrics and Gynecology, University of Michigan, Ann Arbor, Michigan. 4. Department of Epidemiology and Biostatistics, Michigan State University, East Lansing, Michigan. 5. Center for Molecular Medicine and Genetics, Wayne State University, Detroit, Michigan. 6. Department of Obstetrics and Gynecology, Faculty of Medicine, Pontificia Universidad Católica de Chile, Santiago, Chile. 7. Department of Physiology, Wayne State University School of Medicine, Detroit, Michigan. 8. Department of Pathology, University of Massachusetts Medical School, Worcester, Massachusetts. 9. C.S. Mott Center for Human Growth and Development, Wayne State University, Detroit, Michigan. 10. Department of Immunology, Microbiology and Biochemistry, Wayne State University School of Medicine, Detroit, Michigan.
Abstract
PROBLEM: The immunophenotype of B cells at the maternal-fetal interface (decidua) in labor at term and preterm labor is poorly understood. METHOD OF STUDY: Decidual tissues were obtained from women with preterm or term labor and from non-labor gestational age-matched controls. Immunophenotyping of decidual B cells was performed using multicolor flow cytometry. RESULTS: (a) In the absence of acute or chronic chorioamnionitis, total B cells were more abundant in the decidua parietalis of women who delivered preterm than in those who delivered at term, regardless of the presence of labor; (b) decidual transitional and naïve B cells were the most abundant B-cell subsets; (c) decidual B1 B cells were increased in women with either labor at term or preterm labor and chronic chorioamnionitis compared to those without this placental lesion; (d) decidual transitional B cells were reduced in women with preterm labor compared to those without labor; (e) naïve, class-switched, and non-class-switched B cells in the decidual tissues underwent mild alterations with the process of preterm labor; (f) decidual plasmablasts seemed to increase in women with either labor at term or preterm labor with chronic chorioamnionitis; and (g) decidual B cells expressed high levels of interleukin (IL)-12, IL-6, and/or IL-35. CONCLUSION: Total B cells are not increased with the presence of preterm or term labor; yet, specific subsets (B1 and plasmablasts) undergo alterations in women with chronic chorioamnionitis. Therefore, B cells are solely implicated in the pathological process of preterm labor in a subset of women with chronic inflammation of the placenta. These findings provide insight into the immunology of the maternal-fetal interface in preterm and term labor.
PROBLEM: The immunophenotype of B cells at the maternal-fetal interface (decidua) in labor at term and preterm labor is poorly understood. METHOD OF STUDY: Decidual tissues were obtained from women with preterm or term labor and from non-labor gestational age-matched controls. Immunophenotyping of decidual B cells was performed using multicolor flow cytometry. RESULTS: (a) In the absence of acute or chronic chorioamnionitis, total B cells were more abundant in the decidua parietalis of women who delivered preterm than in those who delivered at term, regardless of the presence of labor; (b) decidual transitional and naïve B cells were the most abundant B-cell subsets; (c) decidual B1 B cells were increased in women with either labor at term or preterm labor and chronic chorioamnionitis compared to those without this placental lesion; (d) decidual transitional B cells were reduced in women with preterm labor compared to those without labor; (e) naïve, class-switched, and non-class-switched B cells in the decidual tissues underwent mild alterations with the process of preterm labor; (f) decidual plasmablasts seemed to increase in women with either labor at term or preterm labor with chronic chorioamnionitis; and (g) decidual B cells expressed high levels of interleukin (IL)-12, IL-6, and/or IL-35. CONCLUSION: Total B cells are not increased with the presence of preterm or term labor; yet, specific subsets (B1 and plasmablasts) undergo alterations in women with chronic chorioamnionitis. Therefore, B cells are solely implicated in the pathological process of preterm labor in a subset of women with chronic inflammation of the placenta. These findings provide insight into the immunology of the maternal-fetal interface in preterm and term labor.
Keywords:
B1 B cells; chronic chorioamnionitis; funisitis; memory B cells; naïve B cells; placental inflammation; plasmablasts; pregnancy; transitional B cells
Authors: Anne Schumacher; Stefanie Ehrentraut; Markus Scharm; Hongsheng Wang; Roland Hartig; Herbert C Morse; Ana Claudia Zenclussen Journal: Front Immunol Date: 2018-05-15 Impact factor: 7.561
Authors: Diego Catalán; Miguel Andrés Mansilla; Ashley Ferrier; Lilian Soto; Kristine Oleinika; Juan Carlos Aguillón; Octavio Aravena Journal: Front Immunol Date: 2021-04-29 Impact factor: 7.561