Saranya Ramsridhar1, Malathi Narasimhan2. 1. Former Postgraduate, Department of Oral Pathology, Faculty of Dental Sciences, Sri Ramachandra University , Porur, Chennai, India . 2. Professor and Head, Department of Oral Pathology, Faculty of Dental Sciences, Sri Ramachandra University , Porur, Chennai, India .
Abstract
INTRODUCTION: More than 90% of oral cancers are squamous cell carcinomas with oral leukoplakia being the most common potentially malignant disorder. Among the cell types in the stroma, mast cells play an important role in tumourigenesis through various mechanisms. AIM: The present study was aimed at comparing the mast cell count among normal oral mucosa, leukoplakia and Oral squamous cell carcinoma (OSSC) and to evaluate the possible role of mast cells in carcinogenesis. MATERIALS AND METHODS: Mast cell count was assessed immunohistochemically using anti-mast cell tryptase amongst 20 cases of leukoplakia and OSSC each and 10 normal gingival samples. Overall comparison was done using Kruskal Wallis test and intergroup comparison was done using Mann-Whitney U test. RESULTS: The results of the present study showed an increase in mast cell count from normal oral mucosa (Mean: 7.73) to leukoplakia (Mean: 15.11) to squamous cell carcinoma (Mean: 22.73). Comparison of mean number of mast cells amongst three groups (p-value: 0.001) and intergroup comparisons showed statistical significance. CONCLUSION: Mast cells favour malignant transformation and can be used as indicators of disease progression.
INTRODUCTION: More than 90% of oral cancers are squamous cell carcinomas with oral leukoplakia being the most common potentially malignant disorder. Among the cell types in the stroma, mast cells play an important role in tumourigenesis through various mechanisms. AIM: The present study was aimed at comparing the mast cell count among normal oral mucosa, leukoplakia and Oral squamous cell carcinoma (OSSC) and to evaluate the possible role of mast cells in carcinogenesis. MATERIALS AND METHODS: Mast cell count was assessed immunohistochemically using anti-mast cell tryptase amongst 20 cases of leukoplakia and OSSC each and 10 normal gingival samples. Overall comparison was done using Kruskal Wallis test and intergroup comparison was done using Mann-Whitney U test. RESULTS: The results of the present study showed an increase in mast cell count from normal oral mucosa (Mean: 7.73) to leukoplakia (Mean: 15.11) to squamous cell carcinoma (Mean: 22.73). Comparison of mean number of mast cells amongst three groups (p-value: 0.001) and intergroup comparisons showed statistical significance. CONCLUSION: Mast cells favour malignant transformation and can be used as indicators of disease progression.
Authors: Pio Conti; Maria L Castellani; Durasamy Kempuraj; Vincenzo Salini; Jacopo Vecchiet; Stefano Tetè; Filiberto Mastrangelo; Alessandro Perrella; Maria Anna De Lutiis; Michael Tagen; Theoharis C Theoharides Journal: Ann Clin Lab Sci Date: 2007 Impact factor: 1.256