Literature DB >> 2764887

Characterization of isoelectric subspecies of asialo-beta 2-glycoprotein I.

A Gries1, J Nimpf, H Wurm, G M Kostner, T Kenner.   

Abstract

Isoelectric focusing of purified beta 2-glycoprotein I (beta 2-G-I) revealed five major bands with isoelectric points (pI) between 5.1 and 6.1. Neuraminidase treatment decreased the number of bands to two (pI 8.0 and 8.2). The two asialo subfractions of beta 2-G-I were purified by cation-exchange column chromatography. The more basic isoform II was found to have a higher content of lysine. Western-blot analysis of different plasma samples confirmed the heterogeneity of beta 2-G-I in plasma. Plasma treated with neuraminidase showed two bands irrespective of the number of isoforms as well as of the concentration in native plasma. This led us to the conclusion that human plasma beta 2-G-I consists of two isoproteins that are sialylated to different extents.

Entities:  

Mesh：

Substances：

Year:  1989        PMID： 2764887      PMCID： PMC1138700          DOI： 10.1042/bj2600531

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  18 in total

1.  The thiobarbituric acid assay of sialic acids.

Authors:  L WARREN
Journal:  J Biol Chem       Date:  1959-08       Impact factor: 5.157

2.  Gel isoelectric focusing method for specific diagnosis of familial hyperlipoproteinemia type 3.

Authors:  G R Warnick; C Mayfield; J J Albers; W R Hazzard
Journal:  Clin Chem       Date:  1979-02       Impact factor: 8.327

3.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors:  H Towbin; T Staehelin; J Gordon
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

4.  Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.

Authors:  D W Cleveland; S G Fischer; M W Kirschner; U K Laemmli
Journal:  J Biol Chem       Date:  1977-02-10       Impact factor: 5.157

5.  Separation of subfractions of human beta-2-glycoprotein I.

Authors:  J S Finlayson; J F Mushinski
Journal:  Biochim Biophys Acta       Date:  1967-12-12

6.  Characterization of subfractions of beta 2-glycoprotein I: evidence for sialic acid microheterogeneity.

Authors:  I Schousboe
Journal:  Int J Biochem       Date:  1983

7.  A dot-immunobinding assay for monoclonal and other antibodies.

Authors:  R Hawkes; E Niday; J Gordon
Journal:  Anal Biochem       Date:  1982-01-01       Impact factor: 3.365

8.  Serum beta 2-glycoprotein 1 phenotype frequencies in an English population.

Authors:  J Atkin; A T Rundle
Journal:  Humangenetik       Date:  1974-01-22

9.  Binding of beta 2-glycoprotein I to platelets: effect of adenylate cyclase activity.

Authors:  I Schousboe
Journal:  Thromb Res       Date:  1980 Jul 1-15       Impact factor: 3.944

10.  Characterization of the interaction between beta 2-glycoprotein I and mitochondria, platelets, liposomes and bile acids.

Authors:  I Schousboe
Journal:  Int J Biochem       Date:  1983
View more
  8 in total

1.  Microheterogeneity of beta-2 glycoprotein I: implications for binding to anionic phospholipids.

Authors:  T A Brighton; Y P Dai; P J Hogg; C N Chesterman
Journal:  Biochem J       Date:  1999-05-15       Impact factor: 3.857

2.  Membrane binding of beta2-glycoprotein I can be described by a two-state reaction model: an atomic force microscopy and surface plasmon resonance study.

Authors:  Roland Gamsjaeger; Alexander Johs; Anna Gries; Hermann J Gruber; Christoph Romanin; Ruth Prassl; Peter Hinterdorfer
Journal:  Biochem J       Date:  2005-08-01       Impact factor: 3.857

3.  Domain V peptides inhibit beta2-glycoprotein I-mediated mesenteric ischemia/reperfusion-induced tissue damage and inflammation.

Authors:  Sherry D Fleming; Michael R Pope; Sara M Hoffman; Tiffany Moses; Urska Bukovnik; John M Tomich; Lynn M Wagner; Keith M Woods
Journal:  J Immunol       Date:  2010-10-18       Impact factor: 5.422

4.  Human β2-glycoprotein I attenuates mouse intestinal ischemia/reperfusion induced injury and inflammation.

Authors:  Maurizio Tomasi; Yasuaki Hiromasa; Michael R Pope; Sushanth Gudlur; John M Tomich; Sherry D Fleming
Journal:  Mol Immunol       Date:  2012-06-27       Impact factor: 4.407

5.  Identification of a region of beta 2-glycoprotein I critical for lipid binding and anti-cardiolipin antibody cofactor activity.

Authors:  J E Hunt; R J Simpson; S A Krilis
Journal:  Proc Natl Acad Sci U S A       Date:  1993-03-15       Impact factor: 11.205

6.  Hepatitis B virus surface antigen binds to apolipoprotein H.

Authors:  H Mehdi; M J Kaplan; F Y Anlar; X Yang; R Bayer; K Sutherland; M E Peeples
Journal:  J Virol       Date:  1994-04       Impact factor: 5.103

7.  Beta2 glycoprotein I-derived therapeutic peptides induce sFlt-1 secretion to reduce melanoma vascularity and growth.

Authors:  Haley Smalley; Jennifer M Rowe; Fernando Nieto; Jazmin Zeledon; Kellyn Pollard; John M Tomich; Sherry D Fleming
Journal:  Cancer Lett       Date:  2020-09-04       Impact factor: 8.679

8.  Biophysical analysis of the interaction of the serum protein human β2GPI with bacterial lipopolysaccharide.

Authors:  Anna Gries; Ruth Prassl; Satoshi Fukuoka; Manfred Rössle; Yani Kaconis; Lena Heinbockel; Thomas Gutsmann; Klaus Brandenburg
Journal:  FEBS Open Bio       Date:  2014-05-02       Impact factor: 2.693
  8 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.