Juan Wang1,1, Shufang Zhang2,1, Weihua Ni1, Xiaoyu Zhai1, Fei Xie1, Hongyan Yuan1, Sujun Gao3, Guixiang Tai1. 1. Department of Immunology, College of Basic Medical Sciences, Jilin University, Changchun, Jilin, China. 2. Department of Biochemistry, Basic Medical School, Changchun Medical College, Changchun, Jilin, China. 3. Department of Hematology and Oncology, The First Bethune Hospital of Jilin University, Changchun, Jilin, China.
Abstract
BACKGROUND: Mucin 1 (MUC1), as an oncogene, plays an important role in the diagnosis of lung cancer. OBJECTIVE: To establish a double-antibody sandwich enzyme-linked immune sorbent assay (ELISA) kit for the detection of serum MUC1 in lung cancer patients. METHODS: Commercial mouse anti-human MUC1 monoclonal antibody and rabbit anti-human MUC1 polyclonal antibody were used to construct a double-antibody sandwich ELISA kit. The serum MUC1 levels in peripheral blood of lung disease patients and healthy individuals were detected by the double-antibody sandwich ELISA kit and CA15-3 kit, respectively. RESULTS: A double-antibody sandwich ELISA kit was successfully constructed, and the sensitivity was up to 0.5 μ g/l. The cut-off value for the serum MUC1 levels in the peripheral blood was 1.98 μ g/l, the sensitivity was 62.5%, the specificity was 100% and the Youden index was 0.6250 when detected by the double-antibody sandwich ELISA kit, while the sensitivity was 18.75%, the specificity was 100% and the Youden index was 0.1875 when detected by CA15-3 kit. CONCLUSION: The double-antibody sandwich ELISA kit is superior to the CA15-3 kit in the detection of serum MUC1 in lung cancer patients, suggesting an attractive applying of the double-antibody sandwich ELISA kit in the early diagnosis of lung cancer.
BACKGROUND:Mucin 1 (MUC1), as an oncogene, plays an important role in the diagnosis of lung cancer. OBJECTIVE: To establish a double-antibody sandwich enzyme-linked immune sorbent assay (ELISA) kit for the detection of serum MUC1 in lung cancerpatients. METHODS: Commercial mouse anti-humanMUC1 monoclonal antibody and rabbit anti-humanMUC1 polyclonal antibody were used to construct a double-antibody sandwich ELISA kit. The serum MUC1 levels in peripheral blood of lung diseasepatients and healthy individuals were detected by the double-antibody sandwich ELISA kit and CA15-3 kit, respectively. RESULTS: A double-antibody sandwich ELISA kit was successfully constructed, and the sensitivity was up to 0.5 μ g/l. The cut-off value for the serum MUC1 levels in the peripheral blood was 1.98 μ g/l, the sensitivity was 62.5%, the specificity was 100% and the Youden index was 0.6250 when detected by the double-antibody sandwich ELISA kit, while the sensitivity was 18.75%, the specificity was 100% and the Youden index was 0.1875 when detected by CA15-3 kit. CONCLUSION: The double-antibody sandwich ELISA kit is superior to the CA15-3 kit in the detection of serum MUC1 in lung cancerpatients, suggesting an attractive applying of the double-antibody sandwich ELISA kit in the early diagnosis of lung cancer.
Entities:
Keywords:
CA15-3; ELISA; MUC1; anti-MUC1 polyclonal antibody; lung cancer