Yuyin Li1, Ailong Guo1, Yajuan Feng1, Yueying Zhang1, Jianjun Wang1, Lifang Jing1, Yali Yan1, Lei Jing1, Zhenxing Liu1, Long Ma1, Aipo Diao2. 1. Key Lab of Industrial Fermentation Microbiology of the Ministry of Education, School of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China. 2. Key Lab of Industrial Fermentation Microbiology of the Ministry of Education, School of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China. diaoaipo@tust.edu.cn.
Abstract
OBJECTIVES: TMEPAI (transmembrane prostate androgen-induced protein) has been reported to be overexpressed during tumour progression; however, little is known concerning transcriptional mechanisms regulating TMEPAI gene expression. MATERIALS AND METHODS: In this study, the TMEPAI gene promoter has been identified and characterized, and the effects of Sp1 on TMEPAI-induced viability of A549 cells were evaluated, using MTT and colony formation assays. RESULTS: We found that the sequence between -298 and +24 consists of basal promoter activity for TMEPAI. Further analysis indicated that two Sp1-binding sites are crucial for maintaining basal transcriptional activity of the TMEPAI promoter, and chromatin immunoprecipitation assays confirmed direct binding of Sp1 to the TMEPAI promoter. In addition, Sp1 up-regulated TMEPAI protein expression, as well as Sp1 promoting TMEPAI-induced cell proliferation. CONCLUSIONS: These results indicate that the sequence between -298 and +24 consists of the basal promoter activity for TMEPAI. Sp1 promotes TMEPAI expression and contributes to cell proliferation.
OBJECTIVES:TMEPAI (transmembrane prostate androgen-induced protein) has been reported to be overexpressed during tumour progression; however, little is known concerning transcriptional mechanisms regulating TMEPAI gene expression. MATERIALS AND METHODS: In this study, the TMEPAI gene promoter has been identified and characterized, and the effects of Sp1 on TMEPAI-induced viability of A549 cells were evaluated, using MTT and colony formation assays. RESULTS: We found that the sequence between -298 and +24 consists of basal promoter activity for TMEPAI. Further analysis indicated that two Sp1-binding sites are crucial for maintaining basal transcriptional activity of the TMEPAI promoter, and chromatin immunoprecipitation assays confirmed direct binding of Sp1 to the TMEPAI promoter. In addition, Sp1 up-regulated TMEPAI protein expression, as well as Sp1 promoting TMEPAI-induced cell proliferation. CONCLUSIONS: These results indicate that the sequence between -298 and +24 consists of the basal promoter activity for TMEPAI. Sp1 promotes TMEPAI expression and contributes to cell proliferation.
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