Kristina I Boström1, Jiayi Yao2, Pierre J Guihard2, Ana M Blazquez-Medela2, Yucheng Yao3. 1. Division of Cardiology, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095-1679, USA; The Molecular Biology Institute at UCLA, Los Angeles, CA 90095-1570, USA. Electronic address: kbostrom@mednet.ucla.edu. 2. Division of Cardiology, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095-1679, USA. 3. Division of Cardiology, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095-1679, USA; Jonsson Comprehensive Cancer Center at UCLA, Los Angeles, CA 90095, USA. Electronic address: yyao@mednet.ucla.edu.
Abstract
BACKGROUND AND AIMS: Endothelial-mesenchymal transitions (EndMTs) in endothelial cells (ECs) contribute to vascular disease. METHODS: We used ApoE-/- mice fed a high-fat/high-cholesterol diet. RESULTS: We reported evidence of EndMT in atherosclerotic lesions contributing to calcification. Stem cell and mesenchymal markers, including sex-determining region Y-box 2 (Sox2), were upregulated in aortic ECs of fat-fed ApoE-/- mice. Limiting Sox2 decreased marker expression and calcification in ApoE-/- aortas. Furthermore, a complex of serine proteases was upregulated in ApoE-/- aortic ECs. Blockade of these proteases reduced expression of Sox2 and atherosclerotic lesion calcification. CONCLUSIONS: Together, our data suggest that EndMTs contribute to atherosclerotic lesion calcification.
BACKGROUND AND AIMS: Endothelial-mesenchymal transitions (EndMTs) in endothelial cells (ECs) contribute to vascular disease. METHODS: We used ApoE-/- mice fed a high-fat/high-cholesterol diet. RESULTS: We reported evidence of EndMT in atherosclerotic lesions contributing to calcification. Stem cell and mesenchymal markers, including sex-determining region Y-box 2 (Sox2), were upregulated in aortic ECs of fat-fed ApoE-/- mice. Limiting Sox2 decreased marker expression and calcification in ApoE-/- aortas. Furthermore, a complex of serine proteases was upregulated in ApoE-/- aortic ECs. Blockade of these proteases reduced expression of Sox2 and atherosclerotic lesion calcification. CONCLUSIONS: Together, our data suggest that EndMTs contribute to atherosclerotic lesion calcification.
Authors: Yucheng Yao; Medet Jumabay; Albert Ly; Melina Radparvar; Mark R Cubberly; Kristina I Boström Journal: Circ Res Date: 2013-07-12 Impact factor: 17.367
Authors: Matthias Derwall; Rajeev Malhotra; Carol S Lai; Yuko Beppu; Elena Aikawa; Jasbir S Seehra; Warren M Zapol; Kenneth D Bloch; Paul B Yu Journal: Arterioscler Thromb Vasc Biol Date: 2012-01-05 Impact factor: 8.311
Authors: Jiayi Yao; Pierre J Guihard; Ana M Blazquez-Medela; Yina Guo; Jeremiah H Moon; Medet Jumabay; Kristina I Boström; Yucheng Yao Journal: Circ Res Date: 2015-08-11 Impact factor: 17.367
Authors: Jiayi Yao; Xiuju Wu; Daoqin Zhang; Lumin Wang; Li Zhang; Eric X Reynolds; Carlos Hernandez; Kristina I Boström; Yucheng Yao Journal: J Clin Invest Date: 2019-06-24 Impact factor: 14.808
Authors: Zheng Qin; Ruoxi Liao; Yuqin Xiong; Luojia Jiang; Jiameng Li; Liya Wang; Mei Han; Si Sun; Jiwen Geng; Qinbo Yang; Zhuyun Zhang; Yupei Li; Heyue Du; Baihai Su Journal: Ann Transl Med Date: 2021-04