Caroline B van den Berg1, Johannes J Duvekot1, Coşkun Güzel2, Stefan R Hansson3, Thomas G de Leeuw4, Eric A P Steegers1, Johannes Versendaal1, Theo M Luider2, Marcel P Stoop2. 1. Department of Obstetrics and Gynecology, Division of Obstetrics and Prenatal Medicine, Erasmus University Medical Centre, Rotterdam, The Netherlands. 2. Department of Neurology, Neuro-Oncology, Clinical and Cancer Proteomics Laboratory, Erasmus University Medical Centre, Rotterdam, The Netherlands. 3. Department of Obstetrics and Gynecology, Clinical Sciences, Lund University, Lund, Sweden. 4. Department of Anaesthesiology, Erasmus University Medical Centre, Rotterdam, The Netherlands.
Abstract
PURPOSE: To investigate the cerebrospinal fluid (CSF) proteome of patients with preeclampsia (PE) and normotensive pregnant women, in order to provide a better understanding of brain involvement in PE. EXPERIMENTAL DESIGN: Ninety-eight CSF samples (43 women with PE and 55 normotensive controls) were analyzed by LC-MS/MS proteome profiling. CSF was obtained during the spinal puncture before caesarean delivery. RESULTS: Eight proteins were higher abundant and 17 proteins were lower abundant in patients with PE. The most significantly differentially abundant protein was protein AMBP (alpha-1-microglobulin/bikunin precursor). This finding was validated by performing an ELISA experiment (p = 0.002). CONCLUSIONS AND CLINICAL RELEVANCE: The current study showed a clear difference between the protein profiles of CSF from patients with PE and normotensive pregnant women. Protein AMBP is a precursor of a heme-binding protein that counteracts the damaging effects of free hemoglobin, which may be related to the presence of free hemoglobin in CSF. Protein levels showed correlations with clinical symptoms during pregnancy and postpartum. To our knowledge, this is the first LC-MS/MS proteome profiling study on a unique set of CSF samples from (severe) preeclamptic patients and normotensive pregnant women.
PURPOSE: To investigate the cerebrospinal fluid (CSF) proteome of patients with preeclampsia (PE) and normotensive pregnant women, in order to provide a better understanding of brain involvement in PE. EXPERIMENTAL DESIGN: Ninety-eight CSF samples (43 women with PE and 55 normotensive controls) were analyzed by LC-MS/MS proteome profiling. CSF was obtained during the spinal puncture before caesarean delivery. RESULTS: Eight proteins were higher abundant and 17 proteins were lower abundant in patients with PE. The most significantly differentially abundant protein was protein AMBP (alpha-1-microglobulin/bikunin precursor). This finding was validated by performing an ELISA experiment (p = 0.002). CONCLUSIONS AND CLINICAL RELEVANCE: The current study showed a clear difference between the protein profiles of CSF from patients with PE and normotensive pregnant women. Protein AMBP is a precursor of a heme-binding protein that counteracts the damaging effects of free hemoglobin, which may be related to the presence of free hemoglobin in CSF. Protein levels showed correlations with clinical symptoms during pregnancy and postpartum. To our knowledge, this is the first LC-MS/MS proteome profiling study on a unique set of CSF samples from (severe) preeclamptic patients and normotensive pregnant women.
Authors: Emma L van der Ende; Lieke H Meeter; Christoph Stingl; Jeroen G J van Rooij; Marcel P Stoop; Diana A T Nijholt; Raquel Sanchez-Valle; Caroline Graff; Linn Öijerstedt; Murray Grossman; Corey McMillan; Yolande A L Pijnenburg; Robert Laforce; Giuliano Binetti; Luisa Benussi; Roberta Ghidoni; Theo M Luider; Harro Seelaar; John C van Swieten Journal: Ann Clin Transl Neurol Date: 2019-03-07 Impact factor: 4.511
Authors: Coşkun Güzel; Caroline B van den Berg; Johannes J Duvekot; Christoph Stingl; Thierry P P van den Bosch; Marcel van der Weiden; Eric A P Steegers; Regine P M Steegers-Theunissen; Theo M Luider Journal: Proteomics Clin Appl Date: 2018-11-26 Impact factor: 3.494
Authors: Amanda Kristiansson; Magnus Gram; Johan Flygare; Stefan R Hansson; Bo Åkerström; Jill R Storry Journal: Int J Mol Sci Date: 2020-09-30 Impact factor: 5.923