Lucineia Gainski Danielski1, Eduardo Walczewski1, Clovisa Reck de Jesus1, Drielly Florentino1, Amanda Della Giustina1, Mariana Pereira Goldim1, Luiz Alberto Kanis2, Gregório W Pereira3, Volnei D Pereira4, Francine Felisberto1, Fabricia Petronilho5. 1. Laboratory of Clinical and Experimental Pathophysiology, Postgraduate Program in Health Sciences, University of South Santa Catarina, Tubarão, SC, Brazil. 2. Group of Research in Pharmaceutical Technology TECFARMA, Postgraduate Program in Health Sciences, University of South Santa Catarina, Tubarão, SC, Brazil. 3. Department of Pathology, Federal University of São Paulo, Sao Paulo, SP, Brazil. 4. Institute of Pathological Anatomy and Histopathology DI-PREVER, Tubarão, SC, Brazil. 5. Laboratory of Clinical and Experimental Pathophysiology, Postgraduate Program in Health Sciences, University of South Santa Catarina, Tubarão, SC, Brazil. fabricia.petronilho@unisul.br.
Abstract
PURPOSES: The objective of this study was to evaluate the effect of supplementation with vitamin C on intestinal anastomosis healing in malnourished rats. METHODS: Male Wistar rats were divided into three groups: (1) sham, well-nourished rats that received vehicle; (2) FR+Veh, rats that were subjected to food restriction and received vehicle; and (3) FR+VC, rats that were subjected to food restriction and received vitamin C. Four days before surgery, the animals received vitamin C (100 mg/kg/day) via gavage and underwent colon resection with anastomosis in a single plane. The survival rate of rats was monitored until day 7 after surgery. Regarding anastomosis tissues, we examined intra-abdominal adhesion index, hydroxyproline content, collagen density, inflammatory parameters, and oxidative damage to proteins and lipids. RESULTS: Malnutrition decreases body weight and increases mortality; the survival rate was 90 % in group 1, 60 % in group 2, and 80 % in group 3. Vitamin C was able to increase hydroxyproline concentration and density of collagen and decrease the intra-abdominal adhesion index, as well as the infiltration of neutrophils and oxidative damage to proteins in malnourished rats compared to group treated with vehicle. CONCLUSIONS: Preoperative vitamin C supplementation can improve the intestinal anastomosis healing, biochemical alterations, and prolong survival in rats subjected to food restriction.
PURPOSES: The objective of this study was to evaluate the effect of supplementation with vitamin C on intestinal anastomosis healing in malnourished rats. METHODS: Male Wistar rats were divided into three groups: (1) sham, well-nourished rats that received vehicle; (2) FR+Veh, rats that were subjected to food restriction and received vehicle; and (3) FR+VC, rats that were subjected to food restriction and received vitamin C. Four days before surgery, the animals received vitamin C (100 mg/kg/day) via gavage and underwent colon resection with anastomosis in a single plane. The survival rate of rats was monitored until day 7 after surgery. Regarding anastomosis tissues, we examined intra-abdominal adhesion index, hydroxyproline content, collagen density, inflammatory parameters, and oxidative damage to proteins and lipids. RESULTS:Malnutrition decreases body weight and increases mortality; the survival rate was 90 % in group 1, 60 % in group 2, and 80 % in group 3. Vitamin C was able to increase hydroxyproline concentration and density of collagen and decrease the intra-abdominal adhesion index, as well as the infiltration of neutrophils and oxidative damage to proteins in malnourished rats compared to group treated with vehicle. CONCLUSIONS: Preoperative vitamin C supplementation can improve the intestinal anastomosis healing, biochemical alterations, and prolong survival in rats subjected to food restriction.
Entities:
Keywords:
Inflammation; Intestinal anastomosis; Malnutrition; Oxidative stress; Vitamin C
Authors: Charles N Bernstein; Michael Fried; J H Krabshuis; Henry Cohen; R Eliakim; Suleiman Fedail; Richard Gearry; K L Goh; Saheed Hamid; Aamir Ghafor Khan; A W LeMair; Qin Ouyang; J F Rey; Ajit Sood; Flavio Steinwurz; Ole O Thomsen; Alan Thomson; Gillian Watermeyer Journal: Inflamm Bowel Dis Date: 2010-01 Impact factor: 5.325
Authors: Bassem M Mohammed; Bernard J Fisher; Donatas Kraskauskas; Susan Ward; Jennifer S Wayne; Donald F Brophy; Alpha A Fowler; Dorne R Yager; Ramesh Natarajan Journal: Int Wound J Date: 2015-08-20 Impact factor: 3.315
Authors: Katharina Hill-Mündel; Johannes Schlegl; Hans Konrad Biesalski; Sabrina Ehnert; Steffen Schröter; Christian Bahrs; Donatus Nohr; Andreas K Nüssler; Christoph Ihle Journal: J Clin Med Date: 2019-12-26 Impact factor: 4.241