Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Structure of a Complete Mediator-RNA Polymerase II Pre-Initiation Complex.

Literature DB >> 27610567

Structure of a Complete Mediator-RNA Polymerase II Pre-Initiation Complex.

Philip J Robinson¹, Michael J Trnka², David A Bushnell¹, Ralph E Davis¹, Pierre-Jean Mattei¹, Alma L Burlingame², Roger D Kornberg³.

Abstract

A complete, 52-protein, 2.5 million dalton, Mediator-RNA polymerase II pre-initiation complex (Med-PIC) was assembled and analyzed by cryo-electron microscopy and by chemical cross-linking and mass spectrometry. The resulting complete Med-PIC structure reveals two components of functional significance, absent from previous structures, a protein kinase complex and the Mediator-activator interaction region. It thereby shows how the kinase and its target, the C-terminal domain of the polymerase, control Med-PIC interaction and transcription.

Entities: Chemical Disease Gene Mutation Species

Keywords: Mediator complex; Pre-initiation complex; RNA polymerase II carboxy-terminal domain; TFIIH; TFIIK; Transcription; cross-linking; cryo-EM; mass spectrometry

Mesh：

Substances：

Year: 2016 PMID： 27610567 PMCID： PMC5589196 DOI： 10.1016/j.cell.2016.08.050

Source DB: PubMed Journal: Cell ISSN： 0092-8674 Impact factor: 41.582

57 in total

1. Hyperphosphorylation of the C-terminal repeat domain of RNA polymerase II facilitates dissociation of its complex with mediator.

Authors: T Max M Søgaard; Jesper Q Svejstrup
Journal: J Biol Chem Date: 2007-03-21 Impact factor: 5.157

2. Mutations in RNA polymerase II enhance or suppress mutations in GAL4.

Authors: L A Allison; C J Ingles
Journal: Proc Natl Acad Sci U S A Date: 1989-04 Impact factor: 11.205

3. The structural and functional role of Med5 in the yeast Mediator tail module.

Authors: Jenny Béve; Guo-Zhen Hu; Lawrence C Myers; Darius Balciunas; Olivera Werngren; Kjell Hultenby; Rolf Wibom; Hans Ronne; Claes M Gustafsson
Journal: J Biol Chem Date: 2005-10-17 Impact factor: 5.157

4. Identification, structure, and functional requirement of the Mediator submodule Med7N/31.

Authors: Tobias Koschubs; Martin Seizl; Laurent Larivière; Fabian Kurth; Sonja Baumli; Dietmar E Martin; Patrick Cramer
Journal: EMBO J Date: 2008-12-04 Impact factor: 11.598

5. Features and development of Coot.

Authors: P Emsley; B Lohkamp; W G Scott; K Cowtan
Journal: Acta Crystallogr D Biol Crystallogr Date: 2010-03-24

6. Functional redundancy and structural polymorphism in the large subunit of RNA polymerase II.

Authors: M Nonet; D Sweetser; R A Young
Journal: Cell Date: 1987-09-11 Impact factor: 41.582

7. Construction of a set of convenient Saccharomyces cerevisiae strains that are isogenic to S288C.

Authors: F Winston; C Dollard; S L Ricupero-Hovasse
Journal: Yeast Date: 1995-01 Impact factor: 3.239

8. Structural and enzymatic characterization of a purified prohormone-processing enzyme: secreted, soluble Kex2 protease.

Authors: C Brenner; R S Fuller
Journal: Proc Natl Acad Sci U S A Date: 1992-02-01 Impact factor: 11.205

9. The C-terminal domain of Rpb1 functions on other RNA polymerase II subunits.

Authors: Hyunsuk Suh; Dane Z Hazelbaker; Luis M Soares; Stephen Buratowski
Journal: Mol Cell Date: 2013-09-12 Impact factor: 17.970

10. Relationship of CDK-activating kinase and RNA polymerase II CTD kinase TFIIH/TFIIK.

Authors: W J Feaver; J Q Svejstrup; N L Henry; R D Kornberg
Journal: Cell Date: 1994-12-16 Impact factor: 41.582

99 in total

1. Med15B Regulates Acid Stress Response and Tolerance in Candida glabrata by Altering Membrane Lipid Composition.

Authors: Yanli Qi; Hui Liu; Jiayin Yu; Xiulai Chen; Liming Liu
Journal: Appl Environ Microbiol Date: 2017-08-31 Impact factor: 4.792

Review 2. Chemical cross-linking in the structural analysis of protein assemblies.

Authors: Feixia Chu; Daniel T Thornton; Hieu T Nguyen
Journal: Methods Date: 2018-05-30 Impact factor: 3.608

3. Def1 interacts with TFIIH and modulates RNA polymerase II transcription.

Authors: Nivedita Damodaren; Trevor Van Eeuwen; Joanna Zamel; Enrique Lin-Shiao; Nir Kalisman; Kenji Murakami
Journal: Proc Natl Acad Sci U S A Date: 2017-11-27 Impact factor: 11.205

Review 4. Relating Structure and Dynamics in RNA Biology.

Authors: Kevin P Larsen; Junhong Choi; Arjun Prabhakar; Elisabetta Viani Puglisi; Joseph D Puglisi
Journal: Cold Spring Harb Perspect Biol Date: 2019-07-01 Impact factor: 10.005

5. A two-step probing method to compare lysine accessibility across macromolecular complex conformations.

Authors: Andrew J MacRae; Patricia Coltri; Eva Hrabeta-Robinson; Robert J Chalkley; A L Burlingame; Melissa S Jurica
Journal: RNA Biol Date: 2019-06-29 Impact factor: 4.652

6. A Pliable Mediator Acts as a Functional Rather Than an Architectural Bridge between Promoters and Enhancers.

Authors: Laila El Khattabi; Haiyan Zhao; Jens Kalchschmidt; Natalie Young; Seolkyoung Jung; Peter Van Blerkom; Philippe Kieffer-Kwon; Kyong-Rim Kieffer-Kwon; Solji Park; Xiang Wang; Jordan Krebs; Subhash Tripathi; Noboru Sakabe; Débora R Sobreira; Su-Chen Huang; Suhas S P Rao; Nathanael Pruett; Daniel Chauss; Erica Sadler; Andrea Lopez; Marcelo A Nóbrega; Erez Lieberman Aiden; Francisco J Asturias; Rafael Casellas
Journal: Cell Date: 2019-08-08 Impact factor: 41.582