Hyun-Chul Park1, Taek Kyu Jung2, Mi Jin Kim3, Kyung-Sup Yoon4. 1. R&D Center, Saimdang Cosmetics Co., Ltd., 143, Yangcheongsongdae-gil, Ochang-eup, Cheongwon-gu, Cheongju-si, Chungcheongbuk-do, South Korea. Electronic address: seung-hee@hanmail.net. 2. R&D Center, Saimdang Cosmetics Co., Ltd., 143, Yangcheongsongdae-gil, Ochang-eup, Cheongwon-gu, Cheongju-si, Chungcheongbuk-do, South Korea. Electronic address: jungtk@saimdang.co.kr. 3. R&D Center, Saimdang Cosmetics Co., Ltd., 143, Yangcheongsongdae-gil, Ochang-eup, Cheongwon-gu, Cheongju-si, Chungcheongbuk-do, South Korea. Electronic address: kmj75@saimdang.co.kr. 4. R&D Center, Saimdang Cosmetics Co., Ltd., 143, Yangcheongsongdae-gil, Ochang-eup, Cheongwon-gu, Cheongju-si, Chungcheongbuk-do, South Korea. Electronic address: ksyoonjh@hanmail.net.
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE: Cornus walteri Wangerin has been used in oriental traditional medicine for the treatment of antidiarrheal and inflammation. AIM OF THE STUDY: The efficacy of Cornus walteri Wangerin on skin anti-photoaging was investigated. MATERIALS AND METHODS: Hydrolyzed Cornus walteri Wangerin leaf was tested for the anti-photoaging effects against ultraviolet B (UVB)-induced matrix metalloproteinase (MMP)-1, pro-inflammatory cytokines using human reconstituted skin (KeraSkin™-FT) and also tested for elastase activity in vitro. The MMP-1 and pro-inflammatory cytokine levels of the extract were evaluated by enzyme-linked immunosorbent assay (ELISA). RESULTS: The extract of hydrolyzed Cornus walteri Wangerin leaf (CWE) had the elastase inhibitory activity (IC50: 0.457mg/mL). CWE inhibited MMP-1 expression up to 61% in comparison with the control group which was not treated using CWE, but exposed to UVB. CWE also showed an inhibitory effect on releasing pro-inflammatory cytokines (IL-6 and IL-8) in KeraSkin™-FT (30% and 57% inhibition at dose of 50μg/mL, respectively). CONCLUSION: CWE is a promising anti-photoaging agent for the treatment of UVB-induced skin.
ETHNOPHARMACOLOGICAL RELEVANCE: Cornus walteri Wangerin has been used in oriental traditional medicine for the treatment of antidiarrheal and inflammation. AIM OF THE STUDY: The efficacy of Cornus walteri Wangerin on skin anti-photoaging was investigated. MATERIALS AND METHODS: Hydrolyzed Cornus walteri Wangerin leaf was tested for the anti-photoaging effects against ultraviolet B (UVB)-induced matrix metalloproteinase (MMP)-1, pro-inflammatory cytokines using human reconstituted skin (KeraSkin™-FT) and also tested for elastase activity in vitro. The MMP-1 and pro-inflammatory cytokine levels of the extract were evaluated by enzyme-linked immunosorbent assay (ELISA). RESULTS: The extract of hydrolyzed Cornus walteri Wangerin leaf (CWE) had the elastase inhibitory activity (IC50: 0.457mg/mL). CWE inhibited MMP-1 expression up to 61% in comparison with the control group which was not treated using CWE, but exposed to UVB. CWE also showed an inhibitory effect on releasing pro-inflammatory cytokines (IL-6 and IL-8) in KeraSkin™-FT (30% and 57% inhibition at dose of 50μg/mL, respectively). CONCLUSION: CWE is a promising anti-photoaging agent for the treatment of UVB-induced skin.
Authors: Mei Jing Piao; Kyoung Ah Kang; Yea Seong Ryu; Kristina Shilnikova; Jeong Eon Park; Yu Jae Hyun; Ao Xuan Zhen; Hee Kyoung Kang; Young Sang Koh; Mee Jung Ahn; Jin Won Hyun Journal: Mar Drugs Date: 2017-08-25 Impact factor: 5.118
Authors: Seoung Rak Lee; Eunyong Choi; Se Hun Jeon; Xue Yan Zhi; Jae Sik Yu; Seon-Hee Kim; Jeongmi Lee; Ki-Moon Park; Ki Hyun Kim Journal: Molecules Date: 2018-10-23 Impact factor: 4.411