Tran Van Hung1, Takuya Suzuki2. 1. Department of Biofunctional Science and Technology, Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima, Japan. 2. Department of Biofunctional Science and Technology, Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima, Japan takuya@hiroshima-u.ac.jp.
Abstract
BACKGROUND: Dietary fiber (DF) and its fermentation metabolites play an important role in establishing and maintaining intestinal health. OBJECTIVE: This study investigated the effects of fermentable DF, guar gum (GG), and partially hydrolyzed GG (PHGG) on the epithelial tight junction (TJ) barrier and inflammation in a murine model of dextran sodium sulfate (DSS)-induced colitis. METHODS: In Expt. 1, male, 7-wk-old BALB/c mice weighing ∼21 g were fed diets with 0%, 5%, and 10% GG for 12 d and administered distilled water with 2% DSS for 7 d beginning 5 d after the start of feeding. In Expt. 2, mice were provided diets with or without 10% PHGG and GG for 13 d and administered distilled water with 2% DSS for 8 d from 5 d after the start of feeding. In Expt. 3, mice were provided diets with or without 10% PHGG and GG for 14 d without DSS administration. Colitis score, colon TJ proteins, and fecal SCFA concentrations were analyzed. RESULTS: In Expts. 1 and 2, the clinical score in the DSS group was ∼100% greater than that in the DSS+10% GG and PHGG groups on days 12 and 13 (P < 0.01). The DSS+10% GG and PHGG groups showed ∼110%, 60%, 120%, and 110% greater (P < 0.05) expression of occludin and claudin 3, 4, and 7, respectively, in the colon than did the DSS group. The DSS+10% GG and PHGG groups had greater total fecal SCFA concentrations (25.1 and 12.0 mmol/L) than did the DSS group (3.3 mmol/L) on day 9 (P < 0.01). TJ protein expression did not differ between groups in Expt. 3. CONCLUSION: These findings suggest that microbial metabolites of PHGG and GG, and possibly SCFAs, reduce intestinal barrier defects and inflammation in colitic mice.
BACKGROUND: Dietary fiber (DF) and its fermentation metabolites play an important role in establishing and maintaining intestinal health. OBJECTIVE: This study investigated the effects of fermentable DF, guar gum (GG), and partially hydrolyzed GG (PHGG) on the epithelial tight junction (TJ) barrier and inflammation in a murine model of dextran sodium sulfate (DSS)-induced colitis. METHODS: In Expt. 1, male, 7-wk-old BALB/c mice weighing ∼21 g were fed diets with 0%, 5%, and 10% GG for 12 d and administered distilled water with 2% DSS for 7 d beginning 5 d after the start of feeding. In Expt. 2, mice were provided diets with or without 10% PHGG and GG for 13 d and administered distilled water with 2% DSS for 8 d from 5 d after the start of feeding. In Expt. 3, mice were provided diets with or without 10% PHGG and GG for 14 d without DSS administration. Colitis score, colon TJ proteins, and fecal SCFA concentrations were analyzed. RESULTS: In Expts. 1 and 2, the clinical score in the DSS group was ∼100% greater than that in the DSS+10% GG and PHGG groups on days 12 and 13 (P < 0.01). The DSS+10% GG and PHGG groups showed ∼110%, 60%, 120%, and 110% greater (P < 0.05) expression of occludin and claudin 3, 4, and 7, respectively, in the colon than did the DSS group. The DSS+10% GG and PHGG groups had greater total fecal SCFA concentrations (25.1 and 12.0 mmol/L) than did the DSS group (3.3 mmol/L) on day 9 (P < 0.01). TJ protein expression did not differ between groups in Expt. 3. CONCLUSION: These findings suggest that microbial metabolites of PHGG and GG, and possibly SCFAs, reduce intestinal barrier defects and inflammation in coliticmice.
Authors: Henusha D Jhundoo; Tobias Siefen; Alfred Liang; Christoph Schmidt; John Lokhnauth; Brice Moulari; Arnaud Béduneau; Yann Pellequer; Crilles Casper Larsen; Alf Lamprecht Journal: Int J Pharm X Date: 2021-04-24
Authors: Jelle Folkerts; Ralph Stadhouders; Frank A Redegeld; See-Ying Tam; Rudi W Hendriks; Stephen J Galli; Marcus Maurer Journal: Front Immunol Date: 2018-05-29 Impact factor: 7.561