Literature DB >> 2759709

Transformation of the dermatophyte Trichophyton mentagrophytes to hygromycin B resistance.

R Gonzalez1, S Ferrer, J Buesa, D Ramon.   

Abstract

A transformation system for the ringworm-producing dermatophyte Trichophyton mentagrophytes has been developed. The system employs the plasmid pHIS, which contains a bacterial hygromycin B phosphotransferase gene linked to Cochliobolus heterostrophus regulatory sequences (B. G. Turgeon, R. C. Garber, and O. C. Yoder, Mol. Cell. Biol. 7:3297-3305, 1987). This plasmid confers hygromycin B resistance to T. mentagrophytes. The DNA was stably integrated into the fungal genome, and the number and sites of integrations varied among transformants. Transformant clones were capable of infecting guinea pigs. This system opens the way for the molecular genetic analysis of the interaction of T. mentagrophytes with epithelial animal tissues.

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Year:  1989        PMID： 2759709      PMCID： PMC313551          DOI： 10.1128/iai.57.9.2923-2925.1989

Source DB:  PubMed          Journal:  Infect Immun        ISSN： 0019-9567            Impact factor:   3.441


  14 in total

1.  Development of a fungal transformation system based on selection of sequences with promoter activity.

Authors:  B G Turgeon; R C Garber; O C Yoder
Journal:  Mol Cell Biol       Date:  1987-09       Impact factor: 4.272

2.  Analysis of a bacterial hygromycin B resistance gene by transcriptional and translational fusions and by DNA sequencing.

Authors:  K R Kaster; S G Burgett; R N Rao; T D Ingolia
Journal:  Nucleic Acids Res       Date:  1983-10-11       Impact factor: 16.971

3.  Selectable genes for transformation of the fungal plant pathogen Glomerella cingulata f. sp. phaseoli (Colletotrichum lindemuthianum).

Authors:  R J Rodriguez; O C Yoder
Journal:  Gene       Date:  1987       Impact factor: 3.688

4.  Construction of a fusion gene that confers resistance against hygromycin B to mammalian cells in culture.

Authors:  H U Bernard; G Krämmer; W G Röwekamp
Journal:  Exp Cell Res       Date:  1985-05       Impact factor: 3.905

5.  Nucleotide sequence of the hygromycin B phosphotransferase gene from Streptomyces hygroscopicus.

Authors:  M Zalacain; A González; M C Guerrero; R J Mattaliano; F Malpartida; A Jiménez
Journal:  Nucleic Acids Res       Date:  1986-02-25       Impact factor: 16.971

6.  Transformation of Arabidopsis thaliana with Agrobacterium tumefaciens.

Authors:  A M Lloyd; A R Barnason; S G Rogers; M C Byrne; R T Fraley; R B Horsch
Journal:  Science       Date:  1986-10-24       Impact factor: 47.728

7.  Expression of prokaryotic genes for hygromycin B and G418 resistance as dominant-selection markers in mouse L cells.

Authors:  R F Santerre; N E Allen; J N Hobbs; R N Rao; R J Schmidt
Journal:  Gene       Date:  1984-10       Impact factor: 3.688

8.  Genetic and enzymatic basis of hygromycin B resistance in Escherichia coli.

Authors:  R N Rao; N E Allen; J N Hobbs; W E Alborn; H A Kirst; J W Paschal
Journal:  Antimicrob Agents Chemother       Date:  1983-11       Impact factor: 5.191

9.  Molecular cloning and expression in streptomyces lividans of a hygromycin B phosphotransferase gene from Streptomyces hygroscopicus.

Authors:  F Malpartida; M Zalacaín; A Jiménez; J Davies
Journal:  Biochem Biophys Res Commun       Date:  1983-11-30       Impact factor: 3.575

10.  Gene amplification in Aspergillus nidulans by transformation with vectors containing the amdS gene.

Authors:  K Wernars; T Goosen; L M Wennekes; J Visser; C J Bos; H W van den Broek; R F van Gorcom; C A van den Hondel; P H Pouwels
Journal:  Curr Genet       Date:  1985       Impact factor: 3.886
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  10 in total

Review 1.  Generating and testing molecular hypotheses in the dermatophytes.

Authors:  Theodore C White; Brian G Oliver; Yvonne Gräser; Matthew R Henn
Journal:  Eukaryot Cell       Date:  2008-06-06

Review 2.  Pathogenesis of dermatophytosis.

Authors:  Sandy Vermout; Jérémy Tabart; Aline Baldo; Anne Mathy; Bertrand Losson; Bernard Mignon
Journal:  Mycopathologia       Date:  2008-05-14       Impact factor: 2.574

3.  Molecular cloning ofTrichophyton mentagrophytes DNA sequences with promoter activity inEscherichia coli.

Authors:  C Tortajada; S Ferrer; D Ramon
Journal:  World J Microbiol Biotechnol       Date:  1992-03       Impact factor: 3.312

Review 4.  Genetic Manipulations in Dermatophytes.

Authors:  Mohamed Mahdi Alshahni; Tsuyoshi Yamada
Journal:  Mycopathologia       Date:  2016-07-19       Impact factor: 2.574

Review 5.  Allergy and dermatophytes.

Authors:  Judith A Woodfolk
Journal:  Clin Microbiol Rev       Date:  2005-01       Impact factor: 26.132

6.  Sequenced dermatophyte strains: growth rate, conidiation, drug susceptibilities, and virulence in an invertebrate model.

Authors:  Rebecca R Achterman; Adam R Smith; Brian G Oliver; Theodore C White
Journal:  Fungal Genet Biol       Date:  2010-12-07       Impact factor: 3.495

7.  Development of transformation system for Trichophyton rubrum by electroporation of germinated conidia.

Authors:  Anita Dobrowolska; Pawel Staczek
Journal:  Curr Genet       Date:  2009-07-23       Impact factor: 3.886

8.  Development of a transformation system for Trichoderma longibrachiatum and its use for constructing multicopy transformants for the egl1 gene.

Authors:  P Sánchez-Torres; R González; J A Pérez-González; L González-Candelas; D Ramón
Journal:  Appl Microbiol Biotechnol       Date:  1994-06       Impact factor: 4.813

9.  A novel vector-based RNAi method using mouse U6 promoter-driven shRNA expression in the filamentous fungus Blakeslea trispora.

Authors:  Ye Li; Hui Feng; Lihua Jin; Xiulan Xin; Qipeng Yuan
Journal:  Biotechnol Lett       Date:  2021-06-29       Impact factor: 2.461

10.  Auxotrophic mutations of Trichophyton rubrum created by in vitro synthesized Cas9 ribonucleoprotein.

Authors:  Oliver Blechert; Huan Mei; Xiaohui Zang; Hailin Zheng; Guanzhao Liang; Weida Liu
Journal:  BMC Biotechnol       Date:  2020-01-20       Impact factor: 2.563
  10 in total

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