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Literature DB >> 27590337

Identification of the First Riboflavin Catabolic Gene Cluster Isolated from Microbacterium maritypicum G10.

Hui Xu¹, Yindrila Chakrabarty¹, Benjamin Philmus¹, Angad P Mehta¹, Dhananjay Bhandari¹, Hans-Peter Hohmann¹, Tadhg P Begley².

Abstract

Riboflavin is a common cofactor, and its biosynthetic pathway is well characterized. However, its catabolic pathway, despite intriguing hints in a few distinct organisms, has never been established. This article describes the isolation of a Microbacterium maritypicum riboflavin catabolic strain, and the cloning of the riboflavin catabolic genes. RcaA, RcaB, RcaD, and RcaE were overexpressed and biochemically characterized as riboflavin kinase, riboflavin reductase, ribokinase, and riboflavin hydrolase, respectively. Based on these activities, a pathway for riboflavin catabolism is proposed.

Entities: Chemical Disease Species

Keywords: Vitamin B2; catabolism; cloning; enzyme mechanism; flavoprotein; lyase; quorum sensing; reconstitution; riboflavin

Mesh：

Substances：

Year: 2016 PMID： 27590337 PMCID： PMC5095406 DOI： 10.1074/jbc.M116.729871

Source DB: PubMed Journal: J Biol Chem ISSN： 0021-9258 Impact factor: 5.157

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Identification of the First Riboflavin Catabolic Gene Cluster Isolated from Microbacterium maritypicum G10.

1. A bacterial riboflavin hydrolase.

2. Identification of the gene cluster involved in muraymycin biosynthesis from Streptomyces sp. NRRL 30471.

3. 16S ribosomal DNA amplification for phylogenetic study.

4. Bacterial metabolism of thiamine. I. The isolation and characterization of the initial intermediates in the oxidation of thiamine.

Review 5. Pyridoxal phosphate: biosynthesis and catabolism.

6. Bacterial degradation of folic acid.

7. Bacterial metabolism of thiamine. 3. Metabolism of thiamine to 3-(2'-methyl-4'-amino-5'-pyrimidylmethyl)-4-methyl-thiazole-5-acetic acid (thiamine acetic acid) by a flavoprotein isolated from a soil microorganism.

8. [Interspecies conjugation of Escherichia coli-Streptomyces globisporus 1912 using integrative plasmid pSET152 and its derivatives].

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10. Efficient production of lumichrome by Microbacterium sp. strain TPU 3598.

1. Two-Component Flavin-Dependent Riboflavin Monooxygenase Degrades Riboflavin in Devosia riboflavina.

2. Stark Spectroscopy of Lumichrome: A Possible Candidate for Stand-Off Detection of Bacterial Quorum Sensing.

3. Genome Sequence of Microbacterium sp. Strain TPU 3598, a Lumichrome Producer.

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5. Pathogenicity and Metabolites of Purpureocillium lavendulum YMF1.00683 against Meloidogyne incognita.