Literature DB >> 275853

Existence of only a single functional pool of adenosine triphosphate in human erythrocytes.

E Beutler, E Guinto, W Kuhl, F Matsumoto.   

Abstract

The question of whether separate "membrane" and "soluble" pools of ATP exist in erythrocytes has been examined. Phosphoglycerate kinase (EC 2.7.2.3)-derived ("membrane") ATP was labeled by short-term incubation with inorganic [32P]phosphate. Pyruvate kinase (EC 2.7.1.40)-derived ("soluble")ATP is not labeled under these circumstances. The specific activity of the gamma-phosphate of "soluble" ATP was then evaluated by the addition of 2-deoxyglucose and measurement of the specific activity of 2-deoxyglucose-6-[32P]phosphate formed. This specific activity was essentially the same as the overall specific activity of erythrocyte ATP gama-phosphate, indicating that no functional pools of phosphoglycerate kinase-derived and pyruvate kinase-derived ATP exist in erythrocytes.

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Year:  1978        PMID： 275853      PMCID： PMC392657          DOI： 10.1073/pnas.75.6.2825

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  9 in total

1.  Methods for the isolation of glycolytic intermediated by column chromatography with ion exchange resins.

Authors:  G R BARTLETT
Journal:  J Biol Chem       Date:  1959-03       Impact factor: 5.157

2.  Energy metabolism in human erythrocytes: the role of phosphoglycerate kinase in cation transport.

Authors:  G B Segel; S A Feig; B E Glader; A Muller; P Dutcher; D G Nathan
Journal:  Blood       Date:  1975-08       Impact factor: 22.113

3.  The removal of leukocytes and platelets from whole blood.

Authors:  E Beutler; C West; K G Blume
Journal:  J Lab Clin Med       Date:  1976-08

4.  Phosphorus compounds in the human erythrocyte.

Authors:  G R Bartlett
Journal:  Biochim Biophys Acta       Date:  1968-03-11

5.  Organization of enzymes in human erythrocyte membranes.

Authors:  S L Schrier
Journal:  Am J Physiol       Date:  1966-01

6.  The role of membrane phosphoglycerate kinase in the control of glycolytic rate by active cation transport in human red blood cells.

Authors:  J C Parker; J F Hoffman
Journal:  J Gen Physiol       Date:  1967-03       Impact factor: 4.086

7.  Energy metabolism in human erythrocytes. II. Effects of glucose depletion.

Authors:  S A Feig; G B Segel; S B Shohet; D G Nathan
Journal:  J Clin Invest       Date:  1972-06       Impact factor: 14.808

8.  Explanation for the apparent lack of ouabain inhibition of pyruvate production in hemolysates: the "backward" PGK reaction.

Authors:  R K Chillar; E Beutler
Journal:  Blood       Date:  1976-03       Impact factor: 22.113

9.  Membrane compartmentalized ATP and its preferential use by the Na,K-ATPase of human red cell ghosts.

Authors:  F Proverbio; J F Hoffman
Journal:  J Gen Physiol       Date:  1977-05       Impact factor: 4.086
  9 in total
  5 in total

1.  Multiple metabolic pools of phosphoinositides and phosphatidate in human erythrocytes incubated in a medium that permits rapid transmembrane exchange of phosphate.

Authors:  C E King; L R Stephens; P T Hawkins; G R Guy; R H Michell
Journal:  Biochem J       Date:  1987-05-15       Impact factor: 3.857

2.  Quantifying intracellular rates of glycolytic and oxidative ATP production and consumption using extracellular flux measurements.

Authors:  Shona A Mookerjee; Akos A Gerencser; David G Nicholls; Martin D Brand
Journal:  J Biol Chem       Date:  2017-03-07       Impact factor: 5.157

3.  Product-precursor relationships amongst inositol polyphosphates. Incorporation of [32P]Pi into myo-inositol 1,3,4,6-tetrakisphosphate, myo-inositol 1,3,4,5-tetrakisphosphate, myo-inositol 3,4,5,6-tetrakisphosphate and myo-inositol 1,3,4,5,6-pentakisphosphate in intact avian erythrocytes.

Authors:  L R Stephens; C P Downes
Journal:  Biochem J       Date:  1990-01-15       Impact factor: 3.857

4.  Metabolic compensation for profound erythrocyte adenylate kinase deficiency. A hereditary enzyme defect without hemolytic anemia.

Authors:  E Beutler; D Carson; H Dannawi; L Forman; W Kuhl; C West; B Westwood
Journal:  J Clin Invest       Date:  1983-08       Impact factor: 14.808

5.  Characteristic views of E. coli and B. stearothermophilus 30S ribosomal subunits in the electron microscope.

Authors:  M van Heel; M Stöffler-Meilicke
Journal:  EMBO J       Date:  1985-09       Impact factor: 11.598
  5 in total

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