T Myntti1, L Rahkonen1, A Pätäri-Sampo2, M Tikkanen1, T Sorsa3,4, J Juhila5, O Helve6, S Andersson6, J Paavonen1, V Stefanovic1. 1. Department of Obstetrics and Gynecology, University of Helsinki and Helsinki University Hospital, Helsinki, Finland. 2. Department of Bacteriology, University of Helsinki and Helsinki University Hospital, HUSLAB, Helsinki, Finland. 3. Department of Oral and Maxillofacial Diseases, Helsinki University Hospital, Institute of Dentistry, University of Helsinki, Helsinki, Finland. 4. Division of Periodontology, Department of Dental Medicine, Karolinska Institutet, Huddinge, Sweden. 5. Medix Biochemica, Espoo, Finland. 6. Children's Hospital, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.
Abstract
OBJECTIVE: To evaluate the association of amniotic fluid (AF) matrix metalloproteinase-8 (MMP-8) and cathelicidin concentrations with microbial invasion of the amniotic cavity (MIAC) in pregnancies with preterm prelabor rupture of the membranes or intact membranes. STUDY DESIGN: Amniocentesis was performed in 54 singleton pregnancies between 22+0 and 34+2 gestational weeks with suspected intra-amniotic infection. AF-MMP-8 was analysed by immunoassay and AF-cathelicidin by commercial ELISA. Standard biochemical methods, molecular microbiology and culture techniques were used. RESULTS: MIAC was present in 18 (33%) women. The cutoff value for the diagnosis of MIAC was 41.5 ng ml-1 for AF-MMP-8, and 11.6 ng ml-1 for AF-cathelicidin. With these cutoff values AF-MMP-8 had a sensitivity of 100%, specificity of 69%, positive predictive value of 62% and negative predictive value of 100% for MIAC. The corresponding values for AF-cathelicidin were 89, 81, 70 and 94%. CONCLUSION: The performance of AF-cathelicidin in the prediction of MIAC is comparable to AF-MMP-8.
OBJECTIVE: To evaluate the association of amniotic fluid (AF) matrix metalloproteinase-8 (MMP-8) and cathelicidin concentrations with microbial invasion of the amniotic cavity (MIAC) in pregnancies with preterm prelabor rupture of the membranes or intact membranes. STUDY DESIGN: Amniocentesis was performed in 54 singleton pregnancies between 22+0 and 34+2 gestational weeks with suspected intra-amniotic infection. AF-MMP-8 was analysed by immunoassay and AF-cathelicidin by commercial ELISA. Standard biochemical methods, molecular microbiology and culture techniques were used. RESULTS: MIAC was present in 18 (33%) women. The cutoff value for the diagnosis of MIAC was 41.5 ng ml-1 for AF-MMP-8, and 11.6 ng ml-1 for AF-cathelicidin. With these cutoff values AF-MMP-8 had a sensitivity of 100%, specificity of 69%, positive predictive value of 62% and negative predictive value of 100% for MIAC. The corresponding values for AF-cathelicidin were 89, 81, 70 and 94%. CONCLUSION: The performance of AF-cathelicidin in the prediction of MIAC is comparable to AF-MMP-8.
Authors: S R Angus; S Y Segel; C D Hsu; G J Locksmith; P Clark; M D Sammel; G A Macones; J F Strauss; S Parry Journal: Am J Obstet Gynecol Date: 2001-11 Impact factor: 8.661
Authors: C Andrew Combs; Michael Gravett; Thomas J Garite; Durlin E Hickok; Jodi Lapidus; Richard Porreco; Julie Rael; Thomas Grove; Terry K Morgan; William Clewell; Hugh Miller; David Luthy; Leonardo Pereira; Michael Nageotte; Peter A Robilio; Stephen Fortunato; Hyagriv Simhan; Jason K Baxter; Erol Amon; Albert Franco; Kenneth Trofatter; Kent Heyborne Journal: Am J Obstet Gynecol Date: 2013-11-23 Impact factor: 8.661
Authors: Tarja Myntti; Leena Rahkonen; Irmeli Nupponen; Anu Pätäri-Sampo; Minna Tikkanen; Timo Sorsa; Juuso Juhila; Sture Andersson; Jorma Paavonen; Vedran Stefanovic Journal: Dis Markers Date: 2017-01-12 Impact factor: 3.434