Literature DB >> 27581017

Analyzing the Tumor Microenvironment by Flow Cytometry.

Yoon Kow Young1, Alicia M Bolt1, Ryuhjin Ahn1, Koren K Mann2.   

Abstract

Flow cytometry is an essential tool for studying the tumor microenvironment. It allows us to quickly quantify and identify multiple cell types in a heterogeneous sample. A brief overview of flow cytometry instrumentation and the appropriate considerations and steps in building a good flow cytometry staining panel are discussed. In addition, a lymphoid tissue and solid tumor leukocyte infiltrate flow cytometry staining protocol and an example of flow cytometry data analysis are presented.

Entities:  

Keywords:  Compensation; Flow cytometry (FCM); Fluorescence minus one (FMO); Fluorescence-activated cell sorting (FACS); Immune cell infiltrate; Intracellular staining; Isotype control; Multi-parameter; Solid tumor; Spillover; Staining panel; Tumor microenvironment

Mesh：

Substances：

Year:  2016        PMID： 27581017     DOI： 10.1007/978-1-4939-3801-8_8

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  12 in total

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Journal:  Cell Mol Immunol       Date:  2018-05-07       Impact factor: 11.530

Review 2.  Assessing Tumor-infiltrating Lymphocytes in Solid Tumors: A Practical Review for Pathologists and Proposal for a Standardized Method From the International Immunooncology Biomarkers Working Group: Part 1: Assessing the Host Immune Response, TILs in Invasive Breast Carcinoma and Ductal Carcinoma In Situ, Metastatic Tumor Deposits and Areas for Further Research.

Authors:  Shona Hendry; Roberto Salgado; Thomas Gevaert; Prudence A Russell; Tom John; Bibhusal Thapa; Michael Christie; Koen van de Vijver; M V Estrada; Paula I Gonzalez-Ericsson; Melinda Sanders; Benjamin Solomon; Cinzia Solinas; Gert G G M Van den Eynden; Yves Allory; Matthias Preusser; Johannes Hainfellner; Giancarlo Pruneri; Andrea Vingiani; Sandra Demaria; Fraser Symmans; Paolo Nuciforo; Laura Comerma; E A Thompson; Sunil Lakhani; Seong-Rim Kim; Stuart Schnitt; Cecile Colpaert; Christos Sotiriou; Stefan J Scherer; Michail Ignatiadis; Sunil Badve; Robert H Pierce; Giuseppe Viale; Nicolas Sirtaine; Frederique Penault-Llorca; Tomohagu Sugie; Susan Fineberg; Soonmyung Paik; Ashok Srinivasan; Andrea Richardson; Yihong Wang; Ewa Chmielik; Jane Brock; Douglas B Johnson; Justin Balko; Stephan Wienert; Veerle Bossuyt; Stefan Michiels; Nils Ternes; Nicole Burchardi; Stephen J Luen; Peter Savas; Frederick Klauschen; Peter H Watson; Brad H Nelson; Carmen Criscitiello; Sandra O'Toole; Denis Larsimont; Roland de Wind; Giuseppe Curigliano; Fabrice André; Magali Lacroix-Triki; Mark van de Vijver; Federico Rojo; Giuseppe Floris; Shahinaz Bedri; Joseph Sparano; David Rimm; Torsten Nielsen; Zuzana Kos; Stephen Hewitt; Baljit Singh; Gelareh Farshid; Sibylle Loibl; Kimberly H Allison; Nadine Tung; Sylvia Adams; Karen Willard-Gallo; Hugo M Horlings; Leena Gandhi; Andre Moreira; Fred Hirsch; Maria V Dieci; Maria Urbanowicz; Iva Brcic; Konstanty Korski; Fabien Gaire; Hartmut Koeppen; Amy Lo; Jennifer Giltnane; Marlon C Rebelatto; Keith E Steele; Jiping Zha; Kenneth Emancipator; Jonathan W Juco; Carsten Denkert; Jorge Reis-Filho; Sherene Loi; Stephen B Fox
Journal:  Adv Anat Pathol       Date:  2017-09       Impact factor: 3.875

Review 3.  Consensus guidelines for the use and interpretation of angiogenesis assays.

Authors:  Patrycja Nowak-Sliwinska; Kari Alitalo; Elizabeth Allen; Andrey Anisimov; Alfred C Aplin; Robert Auerbach; Hellmut G Augustin; David O Bates; Judy R van Beijnum; R Hugh F Bender; Gabriele Bergers; Andreas Bikfalvi; Joyce Bischoff; Barbara C Böck; Peter C Brooks; Federico Bussolino; Bertan Cakir; Peter Carmeliet; Daniel Castranova; Anca M Cimpean; Ondine Cleaver; George Coukos; George E Davis; Michele De Palma; Anna Dimberg; Ruud P M Dings; Valentin Djonov; Andrew C Dudley; Neil P Dufton; Sarah-Maria Fendt; Napoleone Ferrara; Marcus Fruttiger; Dai Fukumura; Bart Ghesquière; Yan Gong; Robert J Griffin; Adrian L Harris; Christopher C W Hughes; Nan W Hultgren; M Luisa Iruela-Arispe; Melita Irving; Rakesh K Jain; Raghu Kalluri; Joanna Kalucka; Robert S Kerbel; Jan Kitajewski; Ingeborg Klaassen; Hynda K Kleinmann; Pieter Koolwijk; Elisabeth Kuczynski; Brenda R Kwak; Koen Marien; Juan M Melero-Martin; Lance L Munn; Roberto F Nicosia; Agnes Noel; Jussi Nurro; Anna-Karin Olsson; Tatiana V Petrova; Kristian Pietras; Roberto Pili; Jeffrey W Pollard; Mark J Post; Paul H A Quax; Gabriel A Rabinovich; Marius Raica; Anna M Randi; Domenico Ribatti; Curzio Ruegg; Reinier O Schlingemann; Stefan Schulte-Merker; Lois E H Smith; Jonathan W Song; Steven A Stacker; Jimmy Stalin; Amber N Stratman; Maureen Van de Velde; Victor W M van Hinsbergh; Peter B Vermeulen; Johannes Waltenberger; Brant M Weinstein; Hong Xin; Bahar Yetkin-Arik; Seppo Yla-Herttuala; Mervin C Yoder; Arjan W Griffioen
Journal:  Angiogenesis       Date:  2018-08       Impact factor: 9.596

4.  Inhalation of Tungsten Metal Particulates Alters the Lung and Bone Microenvironments Following Acute Exposure.

Authors:  Kara Miller; Charlotte M McVeigh; Edward B Barr; Guy W Herbert; Quiteria Jacquez; Russell Hunter; Sebastian Medina; Selita N Lucas; Abdul-Mehdi S Ali; Matthew J Campen; Alicia M Bolt
Journal:  Toxicol Sci       Date:  2021-11-24       Impact factor: 4.109

5.  Nanoparticle-Mediated Trapping of Wnt Family Member 5A in Tumor Microenvironments Enhances Immunotherapy for B-Raf Proto-Oncogene Mutant Melanoma.

Authors:  Qi Liu; Hongda Zhu; Karthik Tiruthani; Limei Shen; Fengqian Chen; Keliang Gao; Xueqiong Zhang; Lin Hou; Degeng Wang; Rihe Liu; Leaf Huang
Journal:  ACS Nano       Date:  2018-01-31       Impact factor: 15.881

6.  Nanocarrier-Mediated Chemo-Immunotherapy Arrested Cancer Progression and Induced Tumor Dormancy in Desmoplastic Melanoma.

Authors:  Qi Liu; Fengqian Chen; Lin Hou; Limei Shen; Xueqiong Zhang; Degeng Wang; Leaf Huang
Journal:  ACS Nano       Date:  2018-07-23       Impact factor: 15.881

7.  Isolation and separation of murine tumor-associated macrophages (TAMs) subpopulations from orthotopic 4T1 breast tumors.

Authors:  Pawel Bieniasz-Krzywiec; Rosa Martín-Pérez; Carla Riera-Domingo; Massimiliano Mazzone
Journal:  STAR Protoc       Date:  2021-04-17

8.  ADGRL4/ELTD1 Expression in Breast Cancer Cells Induces Vascular Normalization and Immune Suppression.

Authors:  Helen Sheldon; Esther Bridges; Ildefonso Silva; Massimo Masiero; David M Favara; Dian Wang; Russell Leek; Cameron Snell; Ioannis Roxanis; Mira Kreuzer; Uzi Gileadi; Francesca M Buffa; Alison Banham; Adrian L Harris
Journal:  Mol Cancer Res       Date:  2021-08-04       Impact factor: 5.852

Review 9.  Challenges and Opportunities in the Statistical Analysis of Multiplex Immunofluorescence Data.

Authors:  Christopher M Wilson; Oscar E Ospina; Mary K Townsend; Jonathan Nguyen; Carlos Moran Segura; Joellen M Schildkraut; Shelley S Tworoger; Lauren C Peres; Brooke L Fridley
Journal:  Cancers (Basel)       Date:  2021-06-17       Impact factor: 6.575

10.  Immune and Stroma Related Genes in Breast Cancer: A Comprehensive Analysis of Tumor Microenvironment Based on the Cancer Genome Atlas (TCGA) Database.

Authors:  Ming Xu; Yu Li; Wenhui Li; Qiuyang Zhao; Qiulei Zhang; Kehao Le; Ziwei Huang; Pengfei Yi
Journal:  Front Med (Lausanne)       Date:  2020-03-05
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