Hiroshi Tanaka1, Naoki Okumura2, Noriko Koizumi2, Chie Sotozono1, Yasuhiro Sumii3, Shigeru Kinoshita4. 1. Department of Ophthalmology, Kyoto Prefectural University of Medicine, Graduate School of Medicine, Kyoto, Japan. 2. Department of Biomedical Engineering, Faculty of Life and Medical Sciences, Doshisha University, Kyotanabe, Japan. 3. Konan Medical, Inc, Nishinomiya, Japan. 4. Department of Frontier Medical Science and Technology for Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan.
Abstract
PURPOSE: To observe the most peripheral region of the corneal endothelial cell (CEC) layer as long as optically recordable by use of a prototype slit-scanning wide-field contact specular microscope and produce a panoramic image to evaluate the variation of CEC density with ageing. DESIGN: Observational case series study. METHODS: This study involved 15 eyes of 15 normal healthy subjects divided into three groups according to age: A (20-40 years), B (41-60 years) and C (>60 years). The corneal endothelial layer of each eye was recorded in a horizontal direction, from nasal to temporal, with a slit-scanning wide-field contact specular microscope (Konan) and endothelial cell density (ECD) in three specific regions (central, mid-peripheral, and peripheral) was automatically calculated via built-in analysis software. RESULTS: Corneal endothelial images from near the surgical limbus to limbus in all eyes were clearly recorded and panoramic images were made by combining still images. ECD in groups A, B and C were 2809±186, 2717±91 and 2580±129 cells/mm2 at the centre, 2902±242, 2772±97 and 2604±187 cells/mm2 at the mid-periphery and 2893±308, 2691±99 and 2533±112 cells/mm2 at the periphery. Significance differences in ECD was found between groups A and C in all regions and groups between B and C at mid-peripheral region. CONCLUSIONS: A prototype slit-scanning wide-field contact specular microscope enabled us to record the endothelial layer from the surgical limbus to limbus of the cornea and compare specific areas among subjects, and showed that ECD in each region of the cornea decreases with ageing. TRIAL REGISTRATION NUMBER: UMIN000021264, Results. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/.
PURPOSE: To observe the most peripheral region of the corneal endothelial cell (CEC) layer as long as optically recordable by use of a prototype slit-scanning wide-field contact specular microscope and produce a panoramic image to evaluate the variation of CEC density with ageing. DESIGN: Observational case series study. METHODS: This study involved 15 eyes of 15 normal healthy subjects divided into three groups according to age: A (20-40 years), B (41-60 years) and C (>60 years). The corneal endothelial layer of each eye was recorded in a horizontal direction, from nasal to temporal, with a slit-scanning wide-field contact specular microscope (Konan) and endothelial cell density (ECD) in three specific regions (central, mid-peripheral, and peripheral) was automatically calculated via built-in analysis software. RESULTS: Corneal endothelial images from near the surgical limbus to limbus in all eyes were clearly recorded and panoramic images were made by combining still images. ECD in groups A, B and C were 2809±186, 2717±91 and 2580±129 cells/mm2 at the centre, 2902±242, 2772±97 and 2604±187 cells/mm2 at the mid-periphery and 2893±308, 2691±99 and 2533±112 cells/mm2 at the periphery. Significance differences in ECD was found between groups A and C in all regions and groups between B and C at mid-peripheral region. CONCLUSIONS: A prototype slit-scanning wide-field contact specular microscope enabled us to record the endothelial layer from the surgical limbus to limbus of the cornea and compare specific areas among subjects, and showed that ECD in each region of the cornea decreases with ageing. TRIAL REGISTRATION NUMBER: UMIN000021264, Results. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/.
Authors: Stephen Wahlig; Gary Hin-Fai Yam; Wesley Chong; Xin-Yi Seah; Viridiana Kocaba; Marcus Ang; Hla Myint Htoon; Tin A Tun; Hon Shing Ong; Jodhbir S Mehta Journal: Transl Vis Sci Technol Date: 2018-09-04 Impact factor: 3.283