M Erdogan1, M Kulaksizoglu2, S Ganidagli3, A Berdeli4. 1. Department of Endocrinology and Metabolic Diseases, Ege University Medical School, 35100, Bornova, Izmir, Turkey. drmerdogan61@hotmail.com. 2. Department of Endocrinology and Metabolic Diseases, Necmettin Erbakan University Medical School, Konya, Turkey. 3. Department of Internal Medicine, Numune Training and Research Hospital, Adana, Turkey. 4. Laboratory of Molecular Medicine, Department of Pediatrics, Ege University Medical School, Bornova, Izmir, Turkey.
Abstract
OBJECTIVE: Hashimoto's disease is a polygenic disorder with complex etiopathogenesis. Apoptosis is proposed as one of its mechanisms. The Fas/Fas ligand cascade represents a major pathway initiating apoptosis. This study aims to evaluate the influence of Fas and FasL gene polymorphism in Hashimoto's thyroiditis in Turkish population. MATERIALS AND METHODS: A total of 112 patients with Hashimoto's thyroiditis and 112 cases of healthy control people were included in this study. The evaluation of genotype for Fas -670 A/G and FasL 843 C/T gene polymorphism was performed by using PCR-RFLP method. RESULTS: The FAS genotype and gene allele frequency distribution did differ between the control group (AA 36.6 %, AG 50.0 %, GG 13.4 %, A 61.6 %, G 38.4 %) and the Hashimoto's thyroiditis patients (AA 21.4 %, AG 50.9 %, GG 27.7 %, A 46.9 %, G 53.1 %) (p < 0.01). The evaluation of FasL genotype and gene allele frequency did not show statistically significant difference between the patient group (CC 27.7 %, CT 45.5 %, TT 26.8 %, C 50.4 %, T 49.6 %) and control group (CC 33.9 %, CT 44.6 %, TT 21,4 %, C 56.3 %, T 43.8 %) (p > 0.05). CONCLUSIONS: Gene polymorphism of Fas and G allele frequency may play a role in the regulation of apoptosis in thyroid autoimmune disorders. There is a need for further studies to clarify the genetic role of apoptosis in HT.
OBJECTIVE:Hashimoto's disease is a polygenic disorder with complex etiopathogenesis. Apoptosis is proposed as one of its mechanisms. The Fas/Fas ligand cascade represents a major pathway initiating apoptosis. This study aims to evaluate the influence of Fas and FasL gene polymorphism in Hashimoto's thyroiditis in Turkish population. MATERIALS AND METHODS: A total of 112 patients with Hashimoto's thyroiditis and 112 cases of healthy control people were included in this study. The evaluation of genotype for Fas -670 A/G and FasL 843 C/T gene polymorphism was performed by using PCR-RFLP method. RESULTS: The FAS genotype and gene allele frequency distribution did differ between the control group (AA 36.6 %, AG 50.0 %, GG 13.4 %, A 61.6 %, G 38.4 %) and the Hashimoto's thyroiditispatients (AA 21.4 %, AG 50.9 %, GG 27.7 %, A 46.9 %, G 53.1 %) (p < 0.01). The evaluation of FasL genotype and gene allele frequency did not show statistically significant difference between the patient group (CC 27.7 %, CT 45.5 %, TT 26.8 %, C 50.4 %, T 49.6 %) and control group (CC 33.9 %, CT 44.6 %, TT 21,4 %, C 56.3 %, T 43.8 %) (p > 0.05). CONCLUSIONS: Gene polymorphism of Fas and G allele frequency may play a role in the regulation of apoptosis in thyroid autoimmune disorders. There is a need for further studies to clarify the genetic role of apoptosis in HT.
Authors: B A Treviño-Talavera; C A Palafox-Sánchez; J F Muñoz-Valle; G Orozco-Barocio; R E Navarro-Hernández; M Vázquez-Del Mercado; I García de la Torre; E Oregon-Romero Journal: Genet Mol Res Date: 2014-07-02
Authors: M Erdogan; M Karadeniz; A Berdeli; S Tamsel; Y Ertan; H Uluer; C Yilmaz; M Tuzun; T Kabalak; A G Ozgen Journal: J Endocrinol Invest Date: 2007-05 Impact factor: 4.256
Authors: A Jabrocka-Hybel; A Skalniak; J Piątkowski; R Turek-Jabrocka; P Vyhouskaya; A Ludwig-Słomczyńska; J Machlowska; P Kapusta; M Małecki; D Pach; M Trofimiuk-Müldner; K Lizis-Kolus; A Hubalewska-Dydejczyk Journal: J Endocrinol Invest Date: 2018-06-21 Impact factor: 4.256