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Literature DB >> 27570696

Tomographic imaging of transparent biological samples using the pyramid phase microscope.

Abstract

We show how a pyramid phase microscope can be used to obtain tomographic information of the spatial variation of refractive index in biological samples using the Radon transform. A method that uses the information provided by the phase microscope for axial and lateral repositioning of the sample when it rotates is also described. Its application to the reconstruction of mouse embryos in the blastocyst stage is demonstrated.

Entities: Disease Species

Keywords: (170.6900) Three-dimensional microscopy; (170.6960) Tomography; (350.5030) Phase

Year: 2016 PMID： 27570696 PMCID： PMC4986812 DOI： 10.1364/BOE.7.003049

Source DB: PubMed Journal: Biomed Opt Express ISSN： 2156-7085 Impact factor: 3.732

Keyword Cloud
References

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Tomographic imaging of transparent biological samples using the pyramid phase microscope.

1. Optical sectioning deep inside live embryos by selective plane illumination microscopy.

2. Fully automated, high speed, tomographic phase object reconstruction using the transport of intensity equation in transmission and reflection configurations.

3. Tomographic phase microscopy.

4. Transport-of-intensity approach to differential interference contrast (TI-DIC) microscopy for quantitative phase imaging.

5. Pyramid phase microscopy.

6. Quantitative phase microscopy of transparent samples using a liquid crystal display.

7. Diffraction optical tomography using a quantitative phase imaging unit.

Review 8. Carbon-based nanomaterials for tissue engineering.

9. Optical projection tomography as a tool for 3D microscopy and gene expression studies.

10. Stain-free quantification of chromosomes in live cells using regularized tomographic phase microscopy.