Yong Chen1, Yayun Chen2, Yeye Shi2, Chengyao Ma2, Xunan Wang2, Yue Li2, Yunjie Miao2, Jianwei Chen2, Xiang Li3. 1. College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, China. Electronic address: achenyong@gmail.com. 2. College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, China. 3. College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, China. Electronic address: lixiang_8182@163.com.
Abstract
CONTEXT: Custard apple (Annona squamosa Linn.) is an edible tropical fruit, and its seeds have been used to treat "malignant sore" (cancer) and other usage as insecticide. A comparison of extraction processes, chemical composition analysis and antitumor activity of A. squamosa seed oil (ASO) were investigated. MATERIALS AND METHODS: The optimal extraction parameters of ASO were established by comparing percolation, soxhlet, ultrasonic and SFE-CO2 extraction methods. The chemical composition of fatty acid and content of total annonaceous acetogenins (ACGs) of ASO was investigated by GC-MS and colorimetric assay, and anti-tumor activity of ASO was tested using H22 xenografts bearing mice. RESULTS: The optimal extraction parameters of ASO were obtained as follows: using soxhlet extraction method with extraction solvent of petroleum ether, temperature of 80°C, and extraction time of 90min. Under these conditions, the yield of ASO was 22.65%. GC-MS analysis results showed that the main chemical compositions of fatty acid of ASO were palmitic acid (9.92%), linoleic acid (20.49%), oleic acid (56.50%) and stearic acid (9.14%). The total ACGs content in ASO was 41.00mg/g. ASO inhibited the growth of H22 tumor cells in mice with a maximum inhibitory rate of 53.54% by oral administration. Furthermore, it was found that ASO exerted an antitumor effect via decreasing interleukin-6 (IL-6), janus kinase (Jak) and phosphorylated signal transducers and activators of transcription (p-Stat3) expression. DISCUSSION AND CONCLUSION: The results demonstrated that ASO suppressed the H22 solid tumor development may due to its main chemical constituents unsaturated fatty acid and ACGs via IL-6/Jak/Stat3 pathway. ASO may be a potential candidate for the treatment of cancer.
CONTEXT: Custard apple (Annona squamosa Linn.) is an edible tropical fruit, and its seeds have been used to treat "malignant sore" (cancer) and other usage as insecticide. A comparison of extraction processes, chemical composition analysis and antitumor activity of A. squamosa seed oil (ASO) were investigated. MATERIALS AND METHODS: The optimal extraction parameters of ASO were established by comparing percolation, soxhlet, ultrasonic and SFE-CO2 extraction methods. The chemical composition of fatty acid and content of total annonaceous acetogenins (ACGs) of ASO was investigated by GC-MS and colorimetric assay, and anti-tumor activity of ASO was tested using H22 xenografts bearing mice. RESULTS: The optimal extraction parameters of ASO were obtained as follows: using soxhlet extraction method with extraction solvent of petroleum ether, temperature of 80°C, and extraction time of 90min. Under these conditions, the yield of ASO was 22.65%. GC-MS analysis results showed that the main chemical compositions of fatty acid of ASO were palmitic acid (9.92%), linoleic acid (20.49%), oleic acid (56.50%) and stearic acid (9.14%). The total ACGs content in ASO was 41.00mg/g. ASO inhibited the growth of H22 tumor cells in mice with a maximum inhibitory rate of 53.54% by oral administration. Furthermore, it was found that ASO exerted an antitumor effect via decreasing interleukin-6 (IL-6), janus kinase (Jak) and phosphorylated signal transducers and activators of transcription (p-Stat3) expression. DISCUSSION AND CONCLUSION: The results demonstrated that ASO suppressed the H22 solid tumor development may due to its main chemical constituents unsaturated fatty acid and ACGs via IL-6/Jak/Stat3 pathway. ASO may be a potential candidate for the treatment of cancer.
Authors: Izabela N Faria Gomes; Renato J Silva-Oliveira; Viviane A Oliveira Silva; Marcela N Rosa; Patrik S Vital; Maria Cristina S Barbosa; Fábio Vieira Dos Santos; João Gabriel M Junqueira; Vanessa G P Severino; Bruno G Oliveira; Wanderson Romão; Rui Manuel Reis; Rosy Iara Maciel de Azambuja Ribeiro Journal: Molecules Date: 2019-11-01 Impact factor: 4.411
Authors: Débora Odília Duarte Leite; Cicera Janaine Camilo; Carla de Fatima Alves Nonato; Natália Kelly Gomes de Carvalho; Gerson Javier Torres Salazar; Selene Maia de Morais; José Galberto Martins da Costa Journal: Foods Date: 2021-09-30
Authors: Marcela N Rosa; Larissa R V E Silva; Giovanna B Longato; Adriane F Evangelista; Izabela N F Gomes; Ana Laura V Alves; Bruno G de Oliveira; Fernanda E Pinto; Wanderson Romão; Allisson R de Rezende; Arali A C Araújo; Lohanna S F M Oliveira; Alessandra A de M Souza; Stephanie C Oliveira; Rosy Iara M de A Ribeiro; Viviane A O Silva; Rui M Reis Journal: Int J Mol Sci Date: 2021-03-25 Impact factor: 5.923