| Literature DB >> 27562956 |
Khalil Ettayebi1, Sue E Crawford1, Kosuke Murakami1, James R Broughman1, Umesh Karandikar1, Victoria R Tenge1, Frederick H Neill1, Sarah E Blutt1, Xi-Lei Zeng1, Lin Qu1, Baijun Kou1, Antone R Opekun2, Douglas Burrin3, David Y Graham4, Sasirekha Ramani1, Robert L Atmar5, Mary K Estes6.
Abstract
The major barrier to research and development of effective interventions for human noroviruses (HuNoVs) has been the lack of a robust and reproducible in vitro cultivation system. HuNoVs are the leading cause of gastroenteritis worldwide. We report the successful cultivation of multiple HuNoV strains in enterocytes in stem cell-derived, nontransformed human intestinal enteroid monolayer cultures. Bile, a critical factor of the intestinal milieu, is required for strain-dependent HuNoV replication. Lack of appropriate histoblood group antigen expression in intestinal cells restricts virus replication, and infectivity is abrogated by inactivation (e.g., irradiation, heating) and serum neutralization. This culture system recapitulates the human intestinal epithelium, permits human host-pathogen studies of previously noncultivatable pathogens, and allows the assessment of methods to prevent and treat HuNoV infections.Entities:
Mesh：
Year: 2016 PMID： 27562956 PMCID： PMC5305121 DOI： 10.1126/science.aaf5211
Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 47.728