Literature DB >> 26677228

Human Enteroids/Colonoids and Intestinal Organoids Functionally Recapitulate Normal Intestinal Physiology and Pathophysiology.

Nicholas C Zachos1, Olga Kovbasnjuk1, Jennifer Foulke-Abel1, Julie In1, Sarah E Blutt2, Hugo R de Jonge3, Mary K Estes2, Mark Donowitz4.   

Abstract

Identification of Lgr5 as the intestinal stem cell marker as well as the growth factors necessary to replicate adult intestinal stem cell division has led to the establishment of the methods to generate "indefinite" ex vivo primary intestinal epithelial cultures, termed "mini-intestines." Primary cultures developed from isolated intestinal crypts or stem cells (termed enteroids/colonoids) and from inducible pluripotent stem cells (termed intestinal organoids) are being applied to study human intestinal physiology and pathophysiology with great expectations for translational applications, including regenerative medicine. Here we discuss the physiologic properties of these cultures, their current use in understanding diarrhea-causing host-pathogen interactions, and potential future applications.
© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

Entities:  

Keywords:  differentiation; enteroid; induced pluripotent stem cell (iPS cell) (iPSC); intestinal epithelium; lgr5; organoid; stem cells; transport

Mesh：

Substances：

Year:  2015        PMID： 26677228      PMCID： PMC4759158          DOI： 10.1074/jbc.R114.635995

Source DB:  PubMed          Journal:  J Biol Chem        ISSN： 0021-9258            Impact factor:   5.486


  58 in total

1.  Functional properties of human embryonic stem cell-derived cardiomyocytes.

Authors:  Katya Dolnikov; Mark Shilkrut; Naama Zeevi-Levin; Asaf Danon; Sharon Gerecht-Nir; Joseph Itskovitz-Eldor; Ofer Binah
Journal:  Ann N Y Acad Sci       Date:  2005-06       Impact factor: 5.691

2.  Bmi1 is expressed in vivo in intestinal stem cells.

Authors:  Eugenio Sangiorgi; Mario R Capecchi
Journal:  Nat Genet       Date:  2008-06-08       Impact factor: 38.330

Review 3.  Stem cell plasticity. Plasticity of epithelial stem cells in tissue regeneration.

Authors:  Cédric Blanpain; Elaine Fuchs
Journal:  Science       Date:  2014-06-12       Impact factor: 47.728

Review 4.  Human enteroids as an ex-vivo model of host-pathogen interactions in the gastrointestinal tract.

Authors:  Jennifer Foulke-Abel; Julie In; Olga Kovbasnjuk; Nicholas C Zachos; Khalil Ettayebi; Sarah E Blutt; Joseph M Hyser; Xi-Lei Zeng; Sue E Crawford; James R Broughman; Mary K Estes; Mark Donowitz
Journal:  Exp Biol Med (Maywood)       Date:  2014-04-09

5.  Human Clostridium difficile infection: inhibition of NHE3 and microbiota profile.

Authors:  Melinda A Engevik; Kristen A Engevik; Mary Beth Yacyshyn; Jiang Wang; Daniel J Hassett; Benjamin Darien; Bruce R Yacyshyn; Roger T Worrell
Journal:  Am J Physiol Gastrointest Liver Physiol       Date:  2014-12-31       Impact factor: 4.052

6.  Directed differentiation of human pluripotent stem cells into intestinal tissue in vitro.

Authors:  Jason R Spence; Christopher N Mayhew; Scott A Rankin; Matthew F Kuhar; Jefferson E Vallance; Kathryn Tolle; Elizabeth E Hoskins; Vladimir V Kalinichenko; Susanne I Wells; Aaron M Zorn; Noah F Shroyer; James M Wells
Journal:  Nature       Date:  2010-12-12       Impact factor: 49.962

7.  Stem cell-derived human intestinal organoids as an infection model for rotaviruses.

Authors:  Stacy R Finkbeiner; Xi-Lei Zeng; Budi Utama; Robert L Atmar; Noah F Shroyer; Mary K Estes
Journal:  MBio       Date:  2012-07-03       Impact factor: 7.867

8.  Transplantation of expanded fetal intestinal progenitors contributes to colon regeneration after injury.

Authors:  Robert P Fordham; Shiro Yui; Nicholas R F Hannan; Christoffer Soendergaard; Alison Madgwick; Pawel J Schweiger; Ole H Nielsen; Ludovic Vallier; Roger A Pedersen; Tetsuya Nakamura; Mamoru Watanabe; Kim B Jensen
Journal:  Cell Stem Cell       Date:  2013-10-17       Impact factor: 24.633

9.  Directed differentiation of human pluripotent stem cells into mature airway epithelia expressing functional CFTR protein.

Authors:  Amy P Wong; Christine E Bear; Stephanie Chin; Peter Pasceri; Tadeo O Thompson; Ling-Jun Huan; Felix Ratjen; James Ellis; Janet Rossant
Journal:  Nat Biotechnol       Date:  2012-09       Impact factor: 54.908

10.  Intestinal crypt homeostasis revealed at single-stem-cell level by in vivo live imaging.

Authors:  Laila Ritsma; Saskia I J Ellenbroek; Anoek Zomer; Hugo J Snippert; Frederic J de Sauvage; Benjamin D Simons; Hans Clevers; Jacco van Rheenen
Journal:  Nature       Date:  2014-02-16       Impact factor: 49.962
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  124 in total

Review 1.  Introduction to the Minireview Series on Modern Technologies for In-cell Biochemistry.

Authors:  Svetlana Lutsenko
Journal:  J Biol Chem       Date:  2015-12-16       Impact factor: 5.157

2.  Cholinergic-induced anion secretion in murine jejunal enteroids involves synergy between muscarinic and nicotinic pathways.

Authors:  Kelli Johnson; Jianyi Yin; Julie G In; Subhash Kulkarni; Pankaj Pasricha; Chung Ming Tse; Mark Donowitz
Journal:  Am J Physiol Cell Physiol       Date:  2020-06-17       Impact factor: 4.249

Review 3.  Physiologically relevant human tissue models for infectious diseases.

Authors:  Melody Mills; Mary K Estes
Journal:  Drug Discov Today       Date:  2016-06-25       Impact factor: 7.851

Review 4.  Intestinal Organoids: New Frontiers in the Study of Intestinal Disease and Physiology.

Authors:  Thomas E Wallach; James R Bayrer
Journal:  J Pediatr Gastroenterol Nutr       Date:  2017-02       Impact factor: 2.839

5.  Long-term flow through human intestinal organoids with the gut organoid flow chip (GOFlowChip).

Authors:  Barkan Sidar; Brittany R Jenkins; Sha Huang; Jason R Spence; Seth T Walk; James N Wilking
Journal:  Lab Chip       Date:  2019-10-09       Impact factor: 6.799

Review 6.  Modeling Host-Pathogen Interactions in the Context of the Microenvironment: Three-Dimensional Cell Culture Comes of Age.

Authors:  Jennifer Barrila; Aurélie Crabbé; Jiseon Yang; Karla Franco; Seth D Nydam; Rebecca J Forsyth; Richard R Davis; Sandhya Gangaraju; C Mark Ott; Carolyn B Coyne; Mina J Bissell; Cheryl A Nickerson
Journal:  Infect Immun       Date:  2018-10-25       Impact factor: 3.441

Review 7.  The Dual Tropism of Noroviruses.

Authors:  Christiane E Wobus
Journal:  J Virol       Date:  2018-07-31       Impact factor: 5.103

8.  Breast Milk Enhances Growth of Enteroids: An Ex Vivo Model of Cell Proliferation.

Authors:  Wyatt E Lanik; Lily Xu; Cliff J Luke; Elise Z Hu; Pranjal Agrawal; Victoria S Liu; Rajesh Kumar; Alexa M Bolock; Congrong Ma; Misty Good
Journal:  J Vis Exp       Date:  2018-02-15       Impact factor: 1.355

9.  Replication of human noroviruses in stem cell-derived human enteroids.

Authors:  Khalil Ettayebi; Sue E Crawford; Kosuke Murakami; James R Broughman; Umesh Karandikar; Victoria R Tenge; Frederick H Neill; Sarah E Blutt; Xi-Lei Zeng; Lin Qu; Baijun Kou; Antone R Opekun; Douglas Burrin; David Y Graham; Sasirekha Ramani; Robert L Atmar; Mary K Estes
Journal:  Science       Date:  2016-08-25       Impact factor: 47.728

10.  Colonic epithelial miR-31 associates with the development of Crohn's phenotypes.

Authors:  Benjamin P Keith; Jasmine B Barrow; Takahiko Toyonaga; Nevzat Kazgan; Michelle Hoffner O'Connor; Neil D Shah; Matthew S Schaner; Elisabeth A Wolber; Omar K Trad; Greg R Gipson; Wendy A Pitman; Matthew Kanke; Shruti J Saxena; Nicole Chaumont; Timothy S Sadiq; Mark J Koruda; Paul A Cotney; Nancy Allbritton; Dimitri G Trembath; Francisco Sylvester; Terrence S Furey; Praveen Sethupathy; Shehzad Z Sheikh
Journal:  JCI Insight       Date:  2018-10-04
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