Nathalie Oulhen1, Andreas Heyland2, Tyler J Carrier3, Vanesa Zazueta-Novoa1, Tara Fresques1, Jessica Laird1, Thomas M Onorato4, Daniel Janies5, Gary Wessel6. 1. Brown University, Molecular Biology, Cell Biology, and Biochemistry, USA. 2. Brown University, Molecular Biology, Cell Biology, and Biochemistry, USA; University of Guelph, Integrative Biology, Canada. Electronic address: aheyland@uoguelph.ca. 3. Brown University, Molecular Biology, Cell Biology, and Biochemistry, USA; University of North Carolina at Charlotte, Department of Biological Sciences, USA. 4. LaGuardia Community College/CUNY, Natural Sciences, USA. 5. University of North Carolina at Charlotte, Department of Bioinformatics and Genomics, USA. 6. Brown University, Molecular Biology, Cell Biology, and Biochemistry, USA. Electronic address: rhet@brown.edu.
Abstract
BACKGROUND: Some metazoa have the capacity to regenerate lost body parts. This phenomenon in adults has been classically described in echinoderms, especially in sea stars (Asteroidea). Sea star bipinnaria larvae can also rapidly and effectively regenerate a complete larva after surgical bisection. Understanding the capacity to reverse cell fates in the larva is important from both a developmental and biomedical perspective; yet, the mechanisms underlying regeneration in echinoderms are poorly understood. RESULTS: Here, we describe the process of bipinnaria regeneration after bisection in the bat star Patiria miniata. We tested transcriptional, translational, and cell proliferation activity after bisection in anterior and posterior bipinnaria halves as well as expression of SRAP, reported as a sea star regeneration associated protease (Vickery et al., 2001b). Moreover, we found several genes whose transcripts increased in abundance following bisection, including: Vasa, dysferlin, vitellogenin 1 and vitellogenin 2. CONCLUSION: These results show a transformation following bisection, especially in the anterior halves, of cell fate reassignment in all three germ layers, with clear and predictable changes. These results define molecular events that accompany the cell fate changes coincident to the regenerative response in echinoderm larvae. Crown
BACKGROUND: Some metazoa have the capacity to regenerate lost body parts. This phenomenon in adults has been classically described in echinoderms, especially in sea stars (Asteroidea). Sea star bipinnaria larvae can also rapidly and effectively regenerate a complete larva after surgical bisection. Understanding the capacity to reverse cell fates in the larva is important from both a developmental and biomedical perspective; yet, the mechanisms underlying regeneration in echinoderms are poorly understood. RESULTS: Here, we describe the process of bipinnaria regeneration after bisection in the bat starPatiria miniata. We tested transcriptional, translational, and cell proliferation activity after bisection in anterior and posterior bipinnaria halves as well as expression of SRAP, reported as a sea star regeneration associated protease (Vickery et al., 2001b). Moreover, we found several genes whose transcripts increased in abundance following bisection, including: Vasa, dysferlin, vitellogenin 1 and vitellogenin 2. CONCLUSION: These results show a transformation following bisection, especially in the anterior halves, of cell fate reassignment in all three germ layers, with clear and predictable changes. These results define molecular events that accompany the cell fate changes coincident to the regenerative response in echinoderm larvae. Crown
Authors: Veronica F Hinman; Albert T Nguyen; R Andrew Cameron; Eric H Davidson Journal: Proc Natl Acad Sci U S A Date: 2003-10-31 Impact factor: 11.205
Authors: Igor Yu Dolmatov; Nadezhda V Kalacheva; Ekaterina S Tkacheva; Alena P Shulga; Eugenia G Zavalnaya; Ekaterina V Shamshurina; Alexander S Girich; Alexey V Boyko; Marina G Eliseikina Journal: Genes (Basel) Date: 2021-08-23 Impact factor: 4.096