Literature DB >> 27554834

Leishmania-FAST15: A rapid, sensitive and low-cost real-time PCR assay for the detection of Leishmania infantum and Leishmania braziliensis kinetoplast DNA in canine blood samples.

Filipe Dantas-Torres1, Kamila Gaudêncio da Silva Sales2, Lidiane Gomes da Silva3, Domenico Otranto4, Luciana Aguiar Figueredo2.   

Abstract

We describe an improved real-time PCR assay (designated as "Leishmania-FAST15") for the detection and quantification of Leishmania infantum and Leishmania braziliensis kinetoplast DNA minicircles in canine blood samples. The analytical sensitivity of this technique is 0.1 fg of DNA, which is equivalent to 0.002 parasite per reaction. This assay uses a small reaction volume (15 μl) and is rapid to perform, with the results being available in less than 34 min. This improved assay might also be suitable for detecting and quantifying L. infantum and L. braziliensis DNA in other tissues, such as bone marrow and lymph nodes.
Copyright © 2016 Elsevier Ltd. All rights reserved.

Entities:  

Keywords:  Blood; Diagnosis; Dogs; Leishmania spp.; Real-time PCR

Mesh：

Substances：

Year:  2016        PMID： 27554834     DOI： 10.1016/j.mcp.2016.08.006

Source DB:  PubMed          Journal:  Mol Cell Probes        ISSN： 0890-8508            Impact factor:   2.365


  10 in total

Review 1.  Molecular Diagnosis of Visceral Leishmaniasis.

Authors:  Shyam Sundar; Om Prakash Singh
Journal:  Mol Diagn Ther       Date:  2018-08       Impact factor: 4.074

Review 2.  Canine visceral leishmaniasis: Diagnosis and management of the reservoir living among us.

Authors:  Bruno L Travi; Anabela Cordeiro-da-Silva; Filipe Dantas-Torres; Guadalupe Miró
Journal:  PLoS Negl Trop Dis       Date:  2018-01-11

3.  Sand fly population dynamics and cutaneous leishmaniasis among soldiers in an Atlantic forest remnant in northeastern Brazil.

Authors:  Filipe Dantas-Torres; Kamila Gaudêncio da Silva Sales; Débora Elienai de Oliveira Miranda; Fernando José da Silva; Luciana Aguiar Figueredo; Fábio Lopes de Melo; Maria Edileuza Felinto de Brito; Maria Sandra Andrade; Sinval Pinto Brandão-Filho
Journal:  PLoS Negl Trop Dis       Date:  2017-02-27

4.  Canine β-defensin-1 (CBD1) gene as a possible marker for Leishmania infantum infection in dogs.

Authors:  Lidiane Gomes da Silva; César Raimundo Lima Costa-Júnior; Carlos Alberto Santiago Figueiredo-Júnior; Tereza Cristina Leal-Balbino; Sergio Crovella; Domenico Otranto; Valdir de Queiroz Balbino; Filipe Dantas-Torres
Journal:  Parasit Vectors       Date:  2017-04-20       Impact factor: 3.876

5.  Determination of genetic diversity of Leishmania species using mini-circle kDNA, in Iran-Iraq countries border.

Authors:  Tahereh Mousavi; Sahar Shokohi; Jahangir Abdi; Razi Naserifar; Mahmoud Ahmadi; Asad Mirzaei
Journal:  Trop Parasitol       Date:  2018-12-27

6.  Fast multiplex real-time PCR assay for simultaneous detection of dog and human blood and Leishmania parasites in sand flies.

Authors:  Kamila Gaudêncio da Silva Sales; Débora Elienai de Oliveira Miranda; Marcelo Henrique Santos Paiva; Luciana Aguiar Figueredo; Domenico Otranto; Filipe Dantas-Torres
Journal:  Parasit Vectors       Date:  2020-04-21       Impact factor: 3.876

7.  Prevalence and incidence of vector-borne pathogens in unprotected dogs in two Brazilian regions.

Authors:  Filipe Dantas-Torres; Luciana Aguiar Figueredo; Kamila Gaudêncio da Silva Sales; Débora Elienai de Oliveira Miranda; Joanna Lúcia de Almeida Alexandre; Yury Yzabella da Silva; Lidiane Gomes da Silva; Guilherme Ribeiro Valle; Vitor Márcio Ribeiro; Domenico Otranto; Katrin Deuster; Matthias Pollmeier; Gertraut Altreuther
Journal:  Parasit Vectors       Date:  2020-04-21       Impact factor: 3.876

8.  Asymptomatic Leishmania infection in blood donors from a major blood bank in Northeastern Brazil: a cross-sectional study.

Authors:  Lucas Portela Silva; Silvia Montenegro; Roberto Werkauser; Kamila Gaudêncio da Silva Sales; Fábia Carla Silva Soares; Vlaudia Maria Assis Costa; Ana Cristina Bezerra; Maria Betania do Amaral Pinto; Suzany Maria Ferreira; Herintha Coeto Neitzke-Abreu; Filipe Dantas-Torres; Manoel Sebastião da Costa Lima Junior
Journal:  Rev Inst Med Trop Sao Paulo       Date:  2020-11-27       Impact factor: 1.846

Review 9.  Real-time PCR applications for diagnosis of leishmaniasis.

Authors:  Luca Galluzzi; Marcello Ceccarelli; Aurora Diotallevi; Michele Menotta; Mauro Magnani
Journal:  Parasit Vectors       Date:  2018-05-02       Impact factor: 3.876

10.  Exploring Leishmania infantum cathepsin as a new molecular marker for phylogenetic relationships and visceral leishmaniasis diagnosis.

Authors:  Ryan Emiliano da Silva; Bruna Matarucco Sampaio; Renata Tonhosolo; Andrea Perei Ra da Costa; Luiz Eduardo da Silva Costa; Fernanda Ap Nieri-Bastos; Márcia Aparecida Sperança; Arlei Marcili
Journal:  BMC Infect Dis       Date:  2019-10-28       Impact factor: 3.090
  10 in total

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