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Literature DB >> 2754430

[3H]5-HT binding in post-mortem human cerebral cortex: methodological considerations.

Abstract

The effects of different membrane preparations and assay conditions on [3H]5-HT binding to post-mortem human cortical tissue was studied. Optimal binding necessitated thorough removal of endogenous 5-HT and this was achieved either by hypotonic lysis or by preincubation of the membranes at 37 degrees C. Calcium chloride (4 mM) increased specific [3H]5-HT binding. The further addition of ascorbic acid (5.7 mM) or ascorbic acid and clorgyline (10 microM) reduced specific [3H]5-HT binding.

Entities: Chemical Disease Species

Mesh：

Substances：
Serotonin

Year: 1989 PMID： 2754430 DOI： 10.1007/BF01247113

Source DB: PubMed Journal: J Neural Transm Gen Sect

17 in total

1. Serotonin and lysergic acid diethylamide binding in rat brain membranes: relationship to postsynaptic serotonin receptors.

Authors: J P Bennett; S H Snyder
Journal: Mol Pharmacol Date: 1976-05 Impact factor: 4.436

2. Deamination of 5-hydroxytryptamine by both forms of monoamine oxidase in the rat brain.

Authors: C J Fowler; K F Tipton
Journal: J Neurochem Date: 1982-03 Impact factor: 5.372

3. Possible hazards in serotonin binding assays in human and rat brain: effects of ascorbate, pargyline, and buffer concentration.

Authors: N Weiner; N Arold; W Wesemann
Journal: J Neurosci Methods Date: 1982-01 Impact factor: 2.390

4. Decreased [3H]serotonin and [3H]spiperone binding consequent to lipid peroxidation in rat cortical membranes.

Authors: S F Muakkassah-Kelly; J W Andresen; J C Shih; P Hochstein
Journal: Biochem Biophys Res Commun Date: 1982-02-11 Impact factor: 3.575

5. Characteristics of central 5-HT receptors and their adaptive changes following intracerebral 5,7-dihydroxytryptamine administration in the rat.

Authors: D L Nelson; A Herbet; S Bourgoin; J Glowinski; M Hamon
Journal: Mol Pharmacol Date: 1978-11 Impact factor: 4.436

6. Regional changes in the concentrations of cerebral monoamines and their metabolites after ethanolamine-O-sulphate induced elevation of brain gamma-aminobutyric acid concentrations.

Authors: C Pycock; R Horton
Journal: Biochem Pharmacol Date: 1978 Impact factor: 5.858

[3H]5-HT binding in post-mortem human cerebral cortex: methodological considerations.

1. Serotonin and lysergic acid diethylamide binding in rat brain membranes: relationship to postsynaptic serotonin receptors.

2. Deamination of 5-hydroxytryptamine by both forms of monoamine oxidase in the rat brain.

3. Possible hazards in serotonin binding assays in human and rat brain: effects of ascorbate, pargyline, and buffer concentration.

4. Decreased [3H]serotonin and [3H]spiperone binding consequent to lipid peroxidation in rat cortical membranes.

5. Characteristics of central 5-HT receptors and their adaptive changes following intracerebral 5,7-dihydroxytryptamine administration in the rat.

6. Regional changes in the concentrations of cerebral monoamines and their metabolites after ethanolamine-O-sulphate induced elevation of brain gamma-aminobutyric acid concentrations.

7. Dual effects of ascorbate on serotonin and spiperone binding in rat cortical membranes.

8. The inhibitory effect of reduced glutathione on the lipid peroxidation of the microsomal fraction and mitochondria.

9. Characterization of a novel 3H-5-hydroxytryptamine binding site subtype in bovine brain membranes.

10. Discrimination of multiple [3H]5-hydroxytryptamine binding sites by the neuroleptic spiperone in rat brain.

1. Brain 5-HT1 binding sites in depressed suicides.