| Literature DB >> 27542489 |
Lu Chen1, Fang Wen2, Ming Li2, Xiaodong Guo2, Songli Li2, Nan Zheng3, Jiaqi Wang4.
Abstract
A fluorescent assay for the rapid, sensitive and specific detection of Aflatoxin B1 (AFB1) was developed in this study. Initially, a DNA/DNA duplex was formed between a fluorescein-labeled AFB1 aptamer and its partially complementary DNA strand containing a quencher moiety, resulting in fluorescence quenching due to the close proximity of fluorophore and quencher. Upon the addition of AFB1, an aptamer/AFB1 complex was generated to release the quencher-modified DNA strand, thus recovered the fluorescence of fluorescein and enabled quantitative detection for AFB1 by monitoring fluorescence enhancement. Under optimized conditions, this assay exhibited a linear response to AFB1 in the range of 5-100ng/mL with a detection limit down to 1.6ng/mL. Trials of this assay in infant rice cereal with satisfactory recovery in the range of 93.0%-106.8%, demonstrate that the new assay could be a potential sensing platform for AFB1 determination in food.Entities:
Keywords: Aflatoxin B(1); Aptamer; Fluorescent; Infant rice cereal; Quenching
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Year: 2016 PMID： 27542489 DOI： 10.1016/j.foodchem.2016.07.148
Source DB: PubMed Journal: Food Chem ISSN： 0308-8146 Impact factor: 7.514