Diagnostic yield of endomicroscopy for dysplasia in primary sclerosing cholangitis associated inflammatory bowel disease: a feasibility study.

BACKGROUND AND STUDY AIMS: Primary sclerosing cholangitis associated inflammatory bowel disease (PSC-IBD) is characterized by a high risk of colorectal dysplasia. Surveillance colonoscopies with random biopsies have doubtful power for dysplasia detection. Our aim was to prospectively investigate the feasibility and efficacy of pCLE in surveillance colonoscopies in patients with PSC-IBD. PATIENTS AND METHODS: Sixty-nine patients with PSC-IBD underwent colonoscopy in 2 steps. On the way from rectum to cecum, the mucosa was inspected with high definition endoscopy (HDE) and random biopsies were taken according to the standard routine. On the way from cecum to rectum, fluorescein-enhanced pCLE and chromoendoscopy were performed. Regions where random biopsies had been taken, as well as visible lesions, were examined with pCLE and targeted biopsies were taken of lesions suspicious for dysplasia. Two investigators, blinded to histology and endoscopy results, analyzed all pCLE videos off-line.
RESULTS: Nineteen biopsies obtained in 13 patients (17 targeted biopsies, 2 random biopsies) revealed the presence of low-grade dysplasia. Thirteen lesions with dysplasia were endoscopically visible but by using pCLE-targeted biopsies, additional endoscopically invisible dysplasias in 4 biopsies obtained from 3 patients were detected. The sensitivity, specificity, and accuracy of pCLE in predicting dysplasia were respectively 89 % (95 % CI: 65 - 98), 96 % (95 % CI: 94 - 97), and 96 % (95 % CI: 94 - 97). pCLE showed a good performance for differentiating neoplastic from non-neoplastic mucosa with negative predictive value of 99 %.
CONCLUSIONS: pCLE in PSC-IBD surveillance is feasible and may be a good complement to HDE. Future research should aim at elucidating whether real-time pCLE is applicable in PSC-IBD surveillance.

Introduction

Patients with inflammatory bowel disease (IBD) are at increased risk for development of dysplasia and colorectal cancer (CRC). Although only 1 % to 2 % of patients with CRC have IBD, the cancer-related mortality in IBD patients is approximately 15 % 1 2. The risk for CRC in IBD increases with duration of colitis, early age of IBD onset, extent of colonic involvement, severity of inflammation, a family history of CRC, and in particular, concurrent primary sclerosing cholangitis (PSC) 3. IBD patients with PSC have a 6-fold increased risk for developing dysplasia or CRC compared to non-PSC-IBD patients 4 5. The cumulative risk for developing CRC in PSC-IBD patients is 33 % at 20 years and 40 % at 30 years compared to 8 % and 18 % in IBD without concomitant liver disease 5 6.

Malignancies are detected at an earlier stage in IBD patients undergoing surveillance colonoscopies, resulting in a better prognosis 8 9. However, taking random biopsies at colonoscopy is time-consuming and the sensitivity for detection of neoplasia is insufficient 10 11 12 13. In contrast to sporadic colorectal cancer, IBD preneoplastic tissue is often flat and multifocal and may not be appreciated in up to one-third of colonoscopies 1 14 15. The dysplasia yield from surveillance colonoscopy can be improved by spraying dyes that highlight subtle changes in the architecture of the colonic mucosa. Indeed, a higher efficacy and accuracy of chromoendoscopy over conventional white light endoscopy in IBD surveillance has been confirmed 16 17 18. On the other hand, narrow band imaging (NBI), which highlights vessel and crypt architecture by using blue and green light of specific wavelengths 19, has not been shown to offer any advantage over conventional colonoscopy in IBD surveillance 19 20. According to the European Crohn’s and Colitis Organization’s (ECCO) guidelines, pan-colonic methylene blue or indigo carmine chromoendoscopy with targeted biopsies of any visible lesions is recommended during surveillance colonoscopy, but in centers where appropriate expertise for chromoendoscopy is not available, random biopsies (4 every 10 cm) should be performed 21.

Confocal laser endomicroscopy (CLE) allows detailed evaluation of suspicious lesions at the cellular level with great detail prior to taking targeted biopsies. Two CLE systems have been developed, one of which is integrated into an endoscope (eCLE, Pentax, Tokyo, Japan) and the other is a probe confocal laser endomicroscopy (pCLE), used through the biopsy channel of the endoscope (Cellvizio, Mauna Kea Technologies, Paris, France). CLE is also often called optical biopsy because the technique offers real-time histologic images of tissue. Only a limited field of view within the mucosa can be visualized and pan-endomicroscopy of the whole colon is not feasible 22. Therefore, macroscopic visualization of suspected areas (e. g., chromoendoscopy [red flag technique]) is useful before performing targeted CLE 23 24 . A pilot study using pCLE during colonoscopic surveillance of 22 patients with longstanding ulcerative colitis has demonstrated that this method is feasible with reasonable diagnostic accuracy 25.

PSC-IBD is suggested to represent a specific IBD phenotype characterized by extensive colitis, low inflammatory activity, right-sided colonic inflammation, and a high risk of CRC 5. Thus, endomicroscopy-targeted biopsies might be a particularly promising method for dysplasia detection in PSC-IBD patients. However, to the best of our knowledge, pCLE together with surveillance colonoscopies has not been previously evaluated in PSC-IBD. The aim of this prospective study was to investigate the feasibility and efficacy of pCLE as a complement to high-definition endoscopy (HDE) and chromoendoscopy with random and targeted biopsy sampling for detection of colonic intraepithelial neoplasia in patients with PSC-IBD.

Patients and methods

Patients

We studied patients diagnosis with PSC according to European Association for the Study of the Liver (EASL) criteria 26 and concomitant IBD who were aged 18 to 75 and previously included in an annual colonoscopy surveillance program. Exclusion criteria were pregnancy, severe concomitant diseases, clinically active IBD at the time of colonoscopy defined as Clinical Colitis Activity Index ≥ 4 or Harvey Bradshaw Index ≥ 4 27 28, previous total colectomy, allergy to fluorescein, and lack of informed consent. Patients with a previous partial colectomy or liver transplantation were included if they met the inclusion criteria. Of 293 PSC patients followed at our department, 75 patients who met the inclusion and exclusion criteria were consecutively recruited between August 2011 and June 2014. Five patients declined to participate in the study and 1 patient was excluded because of insufficient quality of pCLE pictures and documentation. Of the 69 remaining patients, 48 (70 %) had been classified as having ulcerative colitis (UC), 16 (23 %) as having Crohn’s Disease (CD), and 5 (7 %) as having unclassified IBD. For detailed patient characteristics, see Table 1.

Characteristics of 69 patients with PSC-IBD included in the study.

Patient characteristics
Age, median (IQR), yr	44 (22 – 72)
Female (%)	18/69 (26)
Ulcerative colitis (%)	48/69 (70)
Crohn colitis (%)	16/69 (23)
Indeterminate colitis (%)	5/69 (7)
IBD duration, median (IQR), yr	19 (3 – 64)
PSC duration, median (IQR), yr	14 (1 – 34)
Colonoscopy surveillance for more than 10 years (%)	42/69 (61)
Previous dysplasia in colon (%)	7/69 (10)
Previous colon surgery due to dysplasia	1/69 (1)
Previous liver transplantation (%)	6/69 (9)
Previous or actual anti-TNF treatment (%)	3/69 (4)
Azathioprine and/or other immunosuppression use (%)	22/69 (32)
5-ASA use (%)	57/69 (83)
Ursodeoxycholic acid use (%)	42/69 (61)

IQR, interquartile range; yr, years.

Endoscopic equipment

Colonoscopies were performed using the Olympus EVIS EXERA II endoscopy system, incorporating HDE and NBI. Confocal endomicroscopy was performed with the probe-based system (pCLE, Cellvizio-GI, Mauna Kea Technologies, Paris, France). The laser-scanning unit excites light with a wavelength of 488 nm via a fiberoptic mini-probe (ColoFlex type UHD; Mauna Kea Technologies). The probe (diameter 2.5 mm) can be inserted through the biopsy channel of any standard colonoscope for contact with the mucosa. For tissue contrast, we used intravenous fluorescein (2.5 mL to 5 mL, 10 %) 29.

Colonoscopy procedure

Patients underwent colonoscopy under conscious sedation using midazolam combined with alfentanil or under sedation with propofol. All colonoscopies were performed by or under the supervision of a gastroenterologist with extensive experience in chromoendoscopy and endomicroscopy (AD) 30. All patients were investigated in a 2-step fashion: the intubation phase and the withdrawal phase. At the intubation phase of the colonoscopy (from rectum to cecum), we performed a high-definition endoscopic (HDE) evaluation of the colon mucosa (Fig. 1 a). The degree of inflammation was evaluated according to the Mayo Score 31 or Simple Endoscopic Score for Crohn’s Disease (SES-CD) 32. All visible lesions were assessed for size and location but not biopsied during intubation. Visible polypoid lesions within non-inflamed areas were scored according to NBI International Colorectal Endoscopic Classification (NICE) 33. All visible lesions were prospectively classified morphologically according to Paris classification 34. Terminology for reporting findings was modified retrospectively after the SCENIC international consensus was published 35. Quadruple random biopsies were taken from 9 standard locations (3 from the right colon, 3 from the transverse colon and 3 from the left colon). This corresponded to every 10 cm of the colon starting from the rectum and ending at the cecum. At the withdrawal phase, after cecal intubation, fluorescein was administrated intravenously and during the withdrawal phase, all visible lesions were evaluated using chromoendoscopy with indigo carmine (Fig. 1 b). We used standard indigo carmine concentration (0.03 %) for lesion detection and in a few cases, we also used a higher concentration (0.13 %) for better lesion characterization. After HDE/chromoendosopy evaluation, each abnormal lesion was inspected using pCLE and targeted biopsies were taken whenever dysplasia was suspected on HDE/chromoendoscopy and/or pCLE occurred. All targeted biopsies were taken on withdrawal, even from lesions detected on the way in. In all targeted biopsies, pCLE was performed before biopsies were taken for histology. The pCLE evaluation is referred to as “optical biopsy.” We carefully examined mucosa surrounding the lesion with both HDE and pCLE but we took targeted biopsies only if HDE and/or pCLE indicated dysplasia suspicion. We did not take any additional biopsies if surrounding mucosa appeared to be normal using both methods of detection.

Fig. 1

 Two-step colonoscopy procedure a During the intubation phase of colonoscopy (from rectum to cecum) we performed high-definition endoscopic evaluation of the colon mucosa. All visible lesions were assessed for size and location but not biopsied. Random biopsies were taken from 9 predefined locations corresponding to every 10 cm of the colon, starting from the rectum and ending in the cecum. b During the withdrawal phase, all visible lesions were defined using chromoendoscopy. After HDE/chromoendosopy evaluation, each abnormal lesion was inspected using pCLE and targeted biopsies were taken whenever a suspicion of dysplasia in HDE/chromoendoscopy and/or pCLE occurred. All areas where the random biopsies had been obtained during the intubation phase were also inspected with pCLE during the withdrawal phase.

All areas in which random biopsies had been obtained during the intubation phase also were inspected with pCLE during the withdrawal phase and optical biopsies were performed in the adjacent region. Additional targeted biopsies for histology were taken whenever suspicious pathological real-time images were detected by pCLE. All pCLE videos were stored for future reassessment. The size, location, and endoscopic description of all visible lesions were recorded.

pCLE assessment

Evaluation of the endomicroscopy images and film sequences was done according to the recently described pCLE classification 36. Normal crypt architecture was classified as Type 1. Type 2 included both star-shaped luminal crypt openings and irregular size of crypts with normal or reduced number of goblet cells. Irregular, dark epithelium with tubular-shaped crypts and loss of goblet cells was classified as Type 3 36. For statistical analyses, classification was reduced to a dichotomous classification of non-neoplasia (crypt Type 1 and Type 2) vs. neoplasia suspicion (crypt Type 3). The pCLE images recorded during the procedure were reviewed off-line in their original format several months later. Two investigators (AD and AMB), blinded to the endoscopic and histological diagnosis, individually performed a final reassessment of the pCLE videos. After the individual assessment, a final endomicroscopic consensus judgment was obtained after discussion between the 2 investigators. Subsequently, the consensus endomicroscopy result was compared to the histopathologic diagnosis.

Histopathology

Biopsies were fixed in 4 % phosphate buffered formaldehyde, embedded in paraffin and stained with hematoxylin-eosin according to routine procedures. To enhance the dysplasia diagnosis, all biopsies that showed changes suspicious for dysplasia were stained with an immunohistochemical (IHC) panel comprising monoclonal antibodies staining for p53, cytokeratin 7, cytokeratin 20 (Novocastra, Leica Microsystems, Bromma, Sweden), P504S, and Mib-1 (Dako, Glostrup, Denmark) 37 38 39 40. Staining was performed in a semiautomatic staining machine (Ventana ES, Ventana Medical Systems, Cedex, France) according to manufacturers protocol. The histologic evaluation, with complementary IHC when deemed necessary, was made according to the international consensus proposed by Riddell et al. 41 and the Vienna classification 42 43, by gastrointestinal pathologists who were blinded to the endoscopic images. Strong overexpression for p53 and P504S, presence of overexpression for cytokeratin 7, positive staining for cytokeratin 20 in the whole crypts, and increased proliferative activity with the positive staining for Mib-1 were defined as IHC-criteria for the presence of dysplasia. All lesions with suspected dysplasia were also re-assessed by a single expert gastrointestinal pathologist (ÅÖ) to confirm the diagnosis of dysplasia.

Statistics

Descriptive statistics was used to analyze the sensitivity, specificity, and accuracy of pCLE for detecting dysplasia by comparing the assessment of optical and histopathologic biopsies taken from the same location, using histopathology diagnosis as the reference standard. To calculate sensitivity, specificity, and accuracy, a pCLE classification of 1 to 2 was considered nonneoplastic whereas a pCLE classification of 3 was considered neoplastic 36. Interobserver agreement of the pCLE classification was calculated using the Cohen’s kappa coefficient. Interpretation of kappa value was performed according to Viera and Garrett 44.

Results

This was a single-center study approved by Stockholm Regional Ethical Review Board, Stockholm, Sweden (Dnr: 2011/207-31/1). All patients invited to participate in the study signed informed consent. The study protocol has been reviewed and registered at Clinicaltrials.gov (identifier: NCT01880606).

During the intubation phase, we obtained random biopsy samples from 9 standard locations (3 from the right colon, 3 from the transverse colon and 3 from the left colon) in 66 patients. In 3 patients we obtained random biopsies from only 6 locations due to previous colon surgery. For further analysis we choose random biopsies taken from all locations in which the quality of corresponding pCLE sequences was good enough to compare to histopathology (n = 607), and 37 targeted biopsies taken during withdrawal phase (Table 2). Thirteen lesions were identified by HDE/chromoendoscopy only, 17/37 by both pCLE/HDE and 7/37 by pCLE only (Fig. 2). The average examination time was 54 ± 13 minutes (range, 30 minutes to 92 minutes).

Neoplasia characteristics of the lesions detected in the study.

Number of colonoscopies performed	69
Procedures with neoplasia (%)	13/69 (19)
Number of biopsies containing neoplasia	19
Targeted biopsies
Total number of targeted biopsies (HDE only1, pCLE only2, both HDE/pCLE3)	37 (13/7/17)
Containing neoplasia (%)	17/37 (46)
All lesions identified by HDE	30
Containing neoplasia (%)	13/30 (43)
All lesions identified by pCLE	24
Containing neoplasia (%)	17/24 (71)
Lesions identified by both HDE and pCLEc	17
Containing neoplasia (%)	13/17 (76)
Random biopsies
Number of analyzed random biopsy locations	607
Total number of analyzed random biopsies	2428
Containing neoplasia (%)	2 (0.08)
Number of random biopsies classified as dysplasia suspicion by pCLE off-line	17
Containing neoplasia (%)	0
Lesions containing neoplasia
Endoscopically visible lesions with LGD (%)	13/19 (68)
Endoscopically non-visible lesions with LGD (%)	6/19 (32)
HGD	0
CRC	0

CRC, colorectal cancer; HDE, high definition endoscopy; HGD, high-grade dysplasia, LGD, low-grade dysplasia; pCLE, probe-based laser endomicroscopy.

Dysplasia suspicion only in HDE, in pCLE- no dysplasia suspicion.

Dysplasia suspicion only in pCLE, in HDE- no dysplasia suspicion.

Dysplasia suspicion in both: HDE and pCLE.

Fig. 2

 Flowchart showing the final histopathologic assessment of random and targeted biopsies.HDE, high-definition endoscopy; pCLE, probe-based laser endomicroscopy; HDE-identified lesions, dysplasia suspicion only in HDE, in pCLE- no dysplasia suspicion; pCLE-identified lesions, dysplasia suspicion only in pCLE, in HDE- no dysplasia suspicion; HDE + pCLE-identified lesions, dysplasia suspicion in both: HDE and pCLE.

Histopathology revealed the presence of low-grade dysplasia in 19 biopsies obtained from 13 patients (10 UC, 2 CD and 1 unclassified IBD). Thirteen dysplastic lesions were endoscopically visible, 4/19 were detected by pCLE only, and 2/19 were found in random biopsies (Fig. 2). For lesions and dysplasia characteristics, see Table 2 and Table 3. The dysplasia was localized in the right colon in 54 % (7/13) of the patients with confirmed dysplasia. Colonoscopy showed the presence of mild/moderate inflammation in 13/48 patients with UC and 4/16 patients with CD. For detailed inflammation/dysplasia characteristics see Table 4.

Characteristics of endoscopy, endomicroscopy and pathology findings of 37 targeted biopsies in 20 patients and 2 random biopsies showing the presence of low-grade dysplasia (LGD)1.

Case	IBD type	Inflammation at the time of colonoscopy	Targeted biopsies (37)
			Location	Endoscopic findings	pCLE		Histopathology
					Crypt architecture	Vessel architecture
02	UC	Present in rectum,Mayo 1	1. Ascending colon	Irregular surface with indistinct border	3	2	Mild inflammation
			2. Ascending colon	Irregular surface with indistinct border	3	2	Mild inflammation
04	CD	Present in ascending and transverse colonSES-CD 4	1. Ascending colon	Inflamed, superficial elevated surface without ulcers	3	3	Active inflammation
			2. Ascending colon	Inflamed, superficial elevated surface without ulcers	3	3	Active inflammation
			3. Transverse colon	Nonpolypoid, flat lesion > 10 mm with indistinct border, 0-IIb	3	3	LGD
			4. Transverse colon	Nonpolypoid, superficial elevated lesion < 10 mm, 0-IIa	3	2	LGD
09	CD	Present in ascending, transverse and sigmoid colon, SES-CD 8	1. Ascending colon	Affected surface with small ulcers	3	3	Active inflammation, LGD
			2. Transverse colon	Affected surface with small ulcers	3	3	Active inflammation, LGD
10	UC	Absent	1. Ascending colon	Irregular surface with distinct border	2c	2	Mild crypt irregularities
			2. Ascending colon	Irregular surface with distinct border	2c	2	Mild crypt irregularities
12	UC	Absent	1. Ascending colon	Nonpolypoid superficial elevated lesion > 10 mm with indistinct border, 0-IIa, NICE 2	3	3	Tubular adenoma with LGD
			2. Ascending colon	Nonpolypoid superficial elevated lesion > 10 mm with indistinct border, 0-IIa, NICE 2	3	3	Tubular adenoma with LGD
			3. Transverse colon	Polypoid sessile lesion < 10 mm, 0-IIa, NICE 1	2c	3	Hyperplastic polyp
14	UC	Absent	1.Descending colon	Polypoid sessile < 10 mm, Paris 0-Is, NICE 1	2c	2	Hyperplastic polyp
			2. Sigmoid colon	Polypoid sessile < 10 mm, Paris 0-Is, NICE 1	2c	2	Hyperplastic polyp
			3. Sigmoid colon	Polypoid sessile < 10 mm, Paris 0-Is, NICE 1	2c	2	Hyperplastic polyp
16	UC	Absent	1. Transverse colon	Polypoid sessile < 10 mm, Paris 0-Is, NICE 1	2c	2	Hyperplastic polyp
			2.Descending colon	Polypoid sessile < 10 mm, Paris 0-Is, NICE 1	2b	2	Hyperplastic polyp
20	UC	PancolitisMayo 2,	1. Cecum	Absence of vascular pattern erythema, superficial elevated surface without ulcers > 10 mm (0-IIa)	3	3	Active inflammation and LGD
			2. Cecum	Absence of vascular pattern erythema, superficial elevated surface without ulcers > 10 mm (0-IIa)	3	3	Active inflammation and LGD
22	CD	Absent	1. Ascending colon	Polypoid sessile < 10 mm, 0-Is, pit NICE 1	2b	2	Hyperplastic polyp
			2. Hepatic flexure	Polypoid sessile > 10 mm, 0-Is, NICE 1	2 d	2	Hyperplastic polyp
			3. Transverse colon	Polypoid sessile < 10 mm, 0-Is, NICE 1	2c	2	Hyperplastic polyp
			4. Splenic flexure	Polypoid sessile < 10 mm, 0-Is, NICE 1	2b	2	Hyperplastic polyp
26	UC	Absent	1. Cecum	Polypoid sessile < 10 mm, 0-Is, NICE 2	3	2	Tubular adenoma with LGD
32	IBD	Absent	1. Transverse colon	Normal mucosaNo visible lesion	3	3	LGD
33	UC	Absent	1. Transverse colon	Post-inflammatory polyp < 10 mmPolypoid sessile ulcerated	3	3	LGD
			2. Sigmoid colon	Post-inflammatory polyp < 10 mmPolypoid sessile ulcerated	3	3	LGD
			3. Rectum	Post-inflammatory polyp < 10 mmPolypoid sessile ulcerated	3	3	LGD
40	UC	PancolitisMayo 1	1. Cecum	Nonpolypoid flat lesion with indistinct border > 10 mm, 0-IIb	3	3	LGD
43	CD	Absent	1. Transverse colon	Post- inflammatory polyp < 10 mmPolypoid sessile ulcerated	3	3	Mild inflammation
45	UC	Absent	1. Transverse colon	Polypoid sessile < 10 mm, 0-Is, NICE 2	3	3	Tubular adenoma with LGD
51	UC	Present in ascending colon, Mayo 1	1. Ascending colon	Honeycomb scars	3	3	LGD
54	UC	Present in cecum and sigmoid colon, Mayo 1,	1. Cecum	Polypoid sessile < 10 mm, 0-Is, NICE 2	3	3	Tubular adenoma with LGD
61	CD	Absent	1. Cecum	Irregular surface with distinct border	3	3	Atypia without dysplasia
63	UC	Absent	1. Sigmoid colon	Normal mucosa	3	2	No active inflammation, no dysplasia
65	UC	Absent	1. Descending colon	Polypoid sessile < 10 mm, 0-Is, NICE 1	2c	3	Hyperplastic polyp
			Random biopsies (2)
05	UC	Absent	1. Ascending colon	Normal mucosa No visible lesion	2 d	2	LGD
13	UC	Present in descending and sigmoid colon, Mayo 1	1. Descending colon	Faded vascular pattern, erythema,No visible lesion	2 d	2	LGD

Among targeted biopsies 13/37 were targeted with HDE, 17/37 with both HDE and pCLE and 7/37 with pCLE only. 13/30 HDE- targeted biopsies and 17/24 pCLE- targeted biopsies confirmed the presence of low-grade dysplasia (LGD). Endoscopic findings are described according to SCENIC terminology and Paris classification. NICE classification was applied only to visible polypoid lesions without concomitant inflammation. pCLE findings are described according to pCLE classification described by Kuiper et al. Crypt type 3 was assessed as dysplasia suspicion.

CD, Crohn’s disease; HDE, high definition endoscopy; NICE, NBI International Colorectal Endoscopic Classification; pCLE, probe-based laser endomicroscopy; SES-CD, Simple Endoscopic Score for Crohn’s Disease; UC, ulcerative colitis.

Dysplasia and inflammation detected in 69 patients with PSC-IBD.

Dysplasia detected (%)	13/69 (19)
UC (%)	10/48 (21)
In inflamed mucosa (%)	5/10 (50)
In non-inflamed mucosa (%)	5/10 (50)
CD (%)	2/16 (13)
In inflamed mucosa (%)	2/2 (100)
In non-inflamed mucosa (%)	0/2 (0)
Unclassified IBD (%)	1/5 (20)
In inflamed mucosa (%)	0/1 (0)
In non-inflamed mucosa	1/1 (100)
Endoscopically visible colitis (%)	19/69 (28)
UC (%)	13/48 (27)
Mayo 1(%)	12/13 (92)
Mayo 2 (%)	1/13 (8)
CD (%)	4/16 (25)
SES-CD ≤ 5 (%)	3/4 (75)
SES-CD > 5 (%)	1/4 (25)
Unclassified IBD (%)	2/5 (40)

HDE and dysplasia detection

Thirty lesions (polyps or mucosa irregularities) in 16 patients were detected by HDE (Fig. 3). All visible lesions were found during the intubation phase and no additional findings were made with use of indigo carmine chromoendoscopy during the withdrawal phase. Histopathology confirmed the presence of low-grade dysplasia in 13/30 visible lesions (Table 2 and Table 3). The sensitivity, specificity, and accuracy of HDE for detecting dysplasia were 68 % (95 % CI: 43 – 86), 97 % (95 % CI: 96 – 98), and 96 % (95 % CI: 94 – 97), respectively (Table 5).

Fig. 3

 Low-grade dysplasia within visible lesion in the right colon. a Colonoscopy detected a visible lesion in the right colon. b and c pCLE showed elongated, irregular crypts with dark epithelium and fluorescein leakage. d Histopathology revealed epithelial atypia with cigar-shaped nuclei with pseudostratification, magnification 20 ×. e Immunohistochemistry showed stronger labeling for p53 in the dysplastic-appearing area than in surrounding crypts (20 ×), and f extensive labeling for cytokeratin 7 (40 ×).

HDE and pCLE classification of dysplastic lesions.

Measure	HDE		pCLE
	Fraction (%)	95 % CI	Fraction (%)	95 % CI
Accuracy	621/644 (96)	94 – 97	618/644 (96)	94 – 97
Sensitivity	13/19 (68)	43 – 86	17/19 (89)	65 – 98
Specificity	608/625 (97)	96 – 98	601/625 (96)	94 – 97
Positive predictive value	13/30 (43)	26 – 62	17/41 (41)	27 – 58
Negative predictive value	608/614 (99)	98 – 100	601/603 (99)	99 – 100

CI, confidence interval; HDE, high definition endoscopy; pCLE, probe-based confocal endomicroscopy.

pCLE and dysplasia detection

We analyzed altogether 808 pCLE sequences obtained from 644 (607 random and 37 targeted) biopsy locations in 69 patients. A total of 238 280 images were studied (mean 370 images and 29 seconds per biopsy location). Each patient was investigated in a median of 12 sequences, which lasted an average of 4.5 minutes. Suspicion of dysplasia was found by pCLE in 41 biopsy locations in 21 patients (24 pCLE targeted biopsies and 17 random biopsy locations classified as dysplasia suspicion by pCLE off-line). Histopathology confirmed the presence of low-grade dysplasia in 17/41 biopsy locations (all pCLE-targeted biopsies) (Table 3, Fig. 4). The sensitivity, specificity, and accuracy for dysplasia detection were 89 % (95 % CI: 65 – 98), 96 % (95 % CI: 94 – 97), and 96 % (95 % CI: 94 – 97), respectively (Table 5). The interobserver agreement for pCLE classification of neoplasia versus nonneoplasia was moderate with kappa value of 0.546 (95 % CI: 0.434 – 0.659)

Fig. 4

 Low-grade dysplasia within macroscopically normal mucosa in the right colon. a Colonoscopy showed an inactive IBD without epithelial irregularities. b pCLE showed irregular crypts and dark epithelium classified as a dysplasia suspicion (score 3 according to Kuiper). c Histopathology showed the presence of low-grade dysplasia in flat mucosa, magnification 20 ×. d Staining for p53 showed strong overexpression indicating mutated p53 in the dysplastic epithelium (40 ×). e Staining for P405S showed an overexpression (40 ×). f Staining for cytokeratin 7 showed a weak overexpression (40 ×). g Staining for cytokeratin 20 was positive in whole crypts (20 ×). h Staining for Mib-1 (Ki-67) showed an increased proliferative activity (40 ×). Immunohistochemistry panel shows the presence of low-grade dysplasia

Optical biopsies classified as false positive by pCLE

Twenty-four optical biopsies from 10 patients were suspicious for dysplasia, which was not confirmed with histopathology or IHC. In 4 of the 10 patients, active inflammation in the colon was present, as noted on both endoscopy and histopathology. In the remaining 6 patients, the histopathology report described irregular epithelium that did not fulfill criteria for dysplasia (Fig. 5).

Fig. 5

 Epithelial atypia with increased proliferative activity but without the presence of dysplasia in the right colon mucosa. a Colonoscopy showed an inactive IBD without epithelial irregularities. b pCLE showed elongated and irregular crypts with dark epithelium. c Histopathology revealed epithelial atypia (40 ×). d Immunohistochemistry showed weak labeling for p53 and e P405S (40 ×). f Mib-1 staining showed increased proliferative activity (40 ×).

Optical biopsies classified as false negative by pCLE

Two of 19 biopsies were classified as false negative by pCLE. Both of these biopsies were taken randomly (Table 3). In the first false-negative case, pCLE confirmed the presence of irregular epithelium without dysplasia suspicion where endoscopy showed a macroscopically normal mucosa. Histology showed irregular epithelium with discrete immunohistochemical abnormalities finally classified as a low-grade dysplasia. In the second false-negative case, real-time endoscopy showed mild inflammation (Mayo 1). pCLE showed irregular, star-shaped crypts and cellular infiltration, which was interpreted as inflammatory changes. The histopathology in that case showed foci of epithelial atypia within the inflamed area, with some of the atypical cells staining positive for p53 and Mib-1. Final histologic assessment concluded focal atypia indefinite for low-grade dysplasia.

Discussion

To the best of our knowledge, this is the first prospective study evaluating pCLE in a surveillance colonoscopy setting in PSC-IBD. pCLE, as a diagnostic means for detecting dysplasia, was shown to have high sensitivity (89 %), specificity (96 %), and accuracy (96 %).

These results are in line with those obtained by Kiesslich et al., who investigated eCLE in a randomized controlled trial of UC patients without concomitant PSC (n = 153) 45. The aim of that study was to compare chromoendoscopy and eCLE with conventional colonoscopy with random biopsies. The authors showed that the diagnostic yield of neoplasia was increased 4.75-fold by using chromoendoscopy together with eCLE 45. In contrast to that, Wanders et al. recently reported on the limited applicability of chromoendoscopy-guided eCLE in Crohn’s colitis surveillance 46. Although the authors achieved relatively good accuracy (87 %) and specificity (92 %) with chromoendoscopy combined with eCLE for differentiating neoplasia from nonneoplasia, they found a low incidence of dysplastic lesions. The study was terminated early because of frequent failure of the endoscopic equipment.

We chose to compare the efficacy of optical pCLE biopsies with histological biopsies to investigate whether the diagnostic yield of dysplasia detection in PSC-IBD could be increased. The high negative predictive value for dysplasia of 98 % to 99 % in pCLE suggests that pCLE may be a good method for excluding dysplasia without the necessity of random sampling for histology, although 2 of the optical biopsies were false negative. However, the random biopsies were sampled from normal-looking mucosa prior to the acquisition of optical biopsies, and thus, it cannot be ruled out that no dysplasia remained in the mucosa when the optical biopsies were performed in the regions adjacent to where the random biopsies were taken. The false-negative cases represented indefinite or low-grade dysplasia and 1 of them was misinterpreted by pCLE as inflammatory changes.

pCLE was particularly effective in detecting dysplasia in PSC-IBD patients where no visible dysplasia suspected lesions were present. The majority of such changes were localized in the right colon, as anticipated in PSC-IBD 5. By using pCLE as a complement to HDE, it was possible to increase the number of lesions detected. Optical biopsies from 4 locations in 3 patients revealed suspicion for dysplasia, which was later confirmed on histopathology, whereas the mucosa was not considered dysplastic during real-time endoscopic examination. However, the number of false-positive cases also increased with use of pCLE. That was particularly true in cases in which inflammation was present. None of our patients had any symptoms of active disease and the majority (89 %) with endoscopically visible colitis had only mild inflammation, which would have been considered as mucosal healing in many studies 47.

Because mucosal inflammation in IBD is well known to reduce the accuracy of diagnosis of dysplasia, cancer surveillance should always be performed on macroscopically non-inflamed mucosa. That is also the case when using pCLE, for which we have noted that presence of inflammation, manifested by crypt destruction and fluorescein leakage, can easily be misinterpreted as dysplasia. Inflammation frequently leads to reduced goblet cells and irregular epithelium, which are also associated with dysplasia. That observation has been confirmed in other reports. In a pilot study by van den Broek et al., active inflammation was shown to be associated with the crypt type 3 appearance in as many as 18 % to 22 % of the cases 25. We could probably avoid “inflammatory pitfalls” by excluding patients with visible colitis from the study analysis. However, our study did not specifically address whether presence of inflammation influenced dysplasia detection. The aim of the study was to investigate the efficacy of pCLE for detection and prediction of dysplasia. We were aware that inflammatory activity often results in mucosal irregularities that masquerade as dysplastic changes 48 and we wanted to assess if it is possible to overcome this disadvantage with real-time endomicroscopy. In our study, pCLE revealed dysplasia suspicion in 6 of 7 patients with histologically confirmed active inflammation and dysplasia at a location classified by HDE has displaying only active inflammation (Table 3 and Table 4), but because of the study was small and therefore lacked, power firm conclusions cannot be made. Future studies should specifically address whether pCLE can make this distinction.

Our interobserver agreement for pCLE classification of neoplasia versus nonneoplasia (0,546) was lower than in the study recently published by Hoffman et al. 49. In that study, the kappa values for experts examining neoplasia in the colon were 0.80 and for students were 0.74. These differences can be explained by differences in study design. In Hoffman’s study, the participants analyzed 25 representative endomicroscopic images of the colon, hand-selected from the database. In our study, 2 investigators separately analyzed a large number of unselected images from 69 patients in which the quality varied. In several cases histology revealed the presence of both active inflammation and dysplasia at the biopsy location, which could easily be misinterpreted during pCLE analysis and probably influenced the obtained moderate interobserver agreement.

Based on our results, we believe that systematic random sampling with pCLE in a non-inflamed mucosa rather than random biopsies can effectively rule out dysplasia and prevent unnecessary biopsy sampling. pCLE may also be a good way to select appropriate areas for biopsies for detection of dysplastic and early neoplastic lesions in PSC-IBD. Based on our findings, it is tempting to suggest modifications of the surveillance colonoscopy protocol for PSC-IBD patients. Our results suggest that choice of endoscopic screening method should be tailored to patient risk factors. We postulate that extensive pCLE sampling, particularly in the right colon, will increase detection yield and surveillance efficiency in PSC-IBD. However, our results need to be validated by other studies, and preferably multicenter research, before that strategy is applied.

There is a consensus that surveillance and monitoring of IBD patients should be performed using a combination of red-flag techniques, such as chromoendoscopy or autofluorescence imaging, to screen large surfaces of mucosa for areas of interest 50 . Areas targeted with chromoendoscopy could then be further evaluated at the microscopic level, using CLE, to improve the characterization of tissue during the procedure 50. In the study by Kiesslich et al. 45, the diagnostic yield of neoplasia was increased using chromoendoscopy with eCLE rather than conventional colonoscopy, even though the number of biopsy specimens was reduced by half 45.

We did not confirm the efficacy of the red flag technique in PSC-IBD surveillance because adding chromoendoscopy or NBI did not increase the rate of detection of mucosa irregularities. Our findings are in line with results obtained by Kahi et al., who showed only marginal improvement in adenoma detection rates with the addition of chromoendoscopy to HDE 51. In a retrospective analysis of 3242 surveillance colonoscopies, Mooiweer et al. 52 showed that despite compelling evidence from randomized trials, implementation of chromoendoscopy for IBD surveillance did not increase dysplasia detection compared with WLE with targeted and random biopsies. According to the SCENIC international consensus statement on surveillance and management of dysplasia in IBD, chromoendoscopy is suggested when performing surveillance with HDE, although this recommendation is conditional because of its reliance on only 1 relatively small observational study 35 53. In our study, HDE appeared to be sufficient for detection of all visible lesions, but because our protocol of taking all random biopsies at the intubation phase resulted in longer and more careful examinations, the detection rate may have been higher, which may have influenced the results. We decided to put significant effort into identifying as many lesions as possible with HDE during intubation in order to concentrate on pCLE detection during withdrawal. Longer procedure duration is significantly associated with likehood of dysplasia diagnosis. In a study performed by Toruner et al. 54, every additional minute increased the rate of dysplasia diagnosis by 3.5 %.

We are aware that our study protocol can represent a certain degree of disadvantage. The design was chosen with the aim of acquiring both optical and histologic biopsies from the same locations in a reasonable amount of time. The timeframe for pCLE is limited after fluorescein injection. To minimize investigation time, we decided to take only targeted biopsies on withdrawal. The repeated change between pCLE probe and biopsy forceps would have prolonged the examination time in the case of random sampling on withdrawal. In that context, the choice of taking random biopsies on the way in was to make the study feasible.

All random biopsies were taken from mucosa without visible lesions. During withdrawal, we could easily identify the region where random biopsies had been taken and we took long pCLE video sequences from all regions adjacent to random biopsies. Another limitation of our study is the fact that we chose to take random biopsies from 9 predefined locations. We decided to use this description in our protocol to avoid confusion. Predefined locations were easier to follow and it helped to describe lesions for further targeted biopsies taken on withdrawal. We made notes and drawings describing lesion locations in relation to predefined biopsy locations, such as “between biopsy nr 3 and 4”. Our predefined locations practically corresponded to approximately every 10 cm of the colon, starting from the rectum and ending at the cecum, but we realize that this description can be confusing.

Conclusions

In conclusion, pCLE in PSC-IBD surveillance is feasible and has a high diagnostic accuracy and negative predictive value, especially in macroscopically normal mucosa and may be a good complementy to HDE. Future research should aim at elucidating whether real-time pCLE may be applicable in PSC-IBD surveillance.