Literature DB >> 2753864

Pulsed-field gel electrophoresis determination of the genome size of obligate intracellular bacteria belonging to the genera Chlamydia, Rickettsiella, and Porochlamydia.

R Frutos1, M Pages, M Bellis, G Roizes, M Bergoin.   

Abstract

The chromosome length of obligate intracellular procaryotes was determined by pulsed-field gel electrophoresis of intact or NotI- and SfiI-restricted genomes. Sizes averaged 2,100, 1,720, 1,550, 2,650, and 1,450 kilobases for Rickettsiella grylli, Rickettsiella melolonthae, Porochlamydia buthi, Porochlamydia chironomi, and Chlamydia psittaci and Chlamydia trachomatis, respectively. An SfiI restriction map of the R. melolonthae genome was derived.

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Year:  1989        PMID： 2753864      PMCID： PMC210235          DOI： 10.1128/jb.171.8.4511-4513.1989

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  10 in total

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Journal:  J Bacteriol       Date:  1969-06       Impact factor: 3.490

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6.  A simple and rapid method for preparing yeast chromosomes for pulsed field gel electrophoresis.

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Journal:  Nucleic Acids Res       Date:  1987-08-25       Impact factor: 16.971

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Journal:  Br Med Bull       Date:  1983-04       Impact factor: 4.291

8.  Surface projections of a chlamydia-like parasite of midge larvae.

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Journal:  J Bacteriol       Date:  1980-12       Impact factor: 3.490

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10.  An electrophoretic karyotype for yeast.

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  10 in total
  20 in total

1.  Decoupling of genome size and sequence divergence in a symbiotic bacterium.

Authors:  J J Wernegreen; H Ochman; I B Jones; N A Moran
Journal:  J Bacteriol       Date:  2000-07       Impact factor: 3.490

2.  Two-step polymerase chain reactions and restriction endonuclease analyses detect and differentiate ompA DNA of Chlamydia spp.

Authors:  B Kaltenboeck; K G Kousoulas; J Storz
Journal:  J Clin Microbiol       Date:  1992-05       Impact factor: 5.948

3.  Construction of physical and genetic maps of Chlamydia trachomatis serovar L2 by pulsed-field gel electrophoresis.

Authors:  S Birkelund; R S Stephens
Journal:  J Bacteriol       Date:  1992-05       Impact factor: 3.490

4.  Rapid field inversion gel electrophoresis in combination with an rRNA gene probe in the epidemiological evaluation of staphylococci.

Authors:  R V Goering; T D Duensing
Journal:  J Clin Microbiol       Date:  1990-03       Impact factor: 5.948

5.  DNA and protein analyses of tick-derived isolates of Borrelia burgdorferi from California.

Authors:  R B LeFebvre; R S Lane; G C Perng; J A Brown; R C Johnson
Journal:  J Clin Microbiol       Date:  1990-04       Impact factor: 5.948

6.  Genetic variability among Chlamydia trachomatis reference and clinical strains analyzed by pulsed-field gel electrophoresis.

Authors:  P Rodriguez; A Allardet-Servent; B de Barbeyrac; M Ramuz; C Bebear
Journal:  J Clin Microbiol       Date:  1994-12       Impact factor: 5.948

7.  Pulse-field electrophoresis indicates full-length Mycoplasma chromosomes range widely in size.

Authors:  H C Neimark; C S Lange
Journal:  Nucleic Acids Res       Date:  1990-09-25       Impact factor: 16.971

8.  Recombinant Escherichia coli clones expressing Chlamydia trachomatis gene products attach to human endometrial epithelial cells.

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Journal:  Infect Immun       Date:  1991-11       Impact factor: 3.441

9.  Determination of genome sizes of Rickettsia spp. within the spotted fever group, using pulsed-field gel electrophoresis.

Authors:  V Roux; M Drancourt; D Raoult
Journal:  J Bacteriol       Date:  1992-11       Impact factor: 3.490

10.  Genomic DNA fingerprinting by pulsed-field gel electrophoresis as an epidemiological marker for study of nosocomial infections caused by methicillin-resistant Staphylococcus aureus.

Authors:  S Ichiyama; M Ohta; K Shimokata; N Kato; J Takeuchi
Journal:  J Clin Microbiol       Date:  1991-12       Impact factor: 5.948
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