Literature DB >> 1569006

Construction of physical and genetic maps of Chlamydia trachomatis serovar L2 by pulsed-field gel electrophoresis.

S Birkelund1, R S Stephens.   

Abstract

We constructed the physical map of Chlamydia trachomatis serovar L2 by using three restriction endonucleases, NotI (GC[GGCCGC), SgrAI (C(A/G)[CCGG(T/G)G), and Sse8387I (CCTGCA[GG), and we analyzed the fragments by pulsed-field gel electrophoresis. A total of 25 restriction endonuclease sites and 13 genes and/or operons were located on the map. The genome size was determined to be 1,045 kb. Neither highly transcribed chlamydia genes nor developmental cycle-specific genes were clustered on the genome.

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Year:  1992        PMID: 1569006      PMCID: PMC205923          DOI: 10.1128/jb.174.9.2742-2747.1992

Source DB:  PubMed          Journal:  J Bacteriol        ISSN: 0021-9193            Impact factor:   3.490


  30 in total

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Authors:  A G Lundemose; S Birkelund; P M Larsen; S J Fey; G Christiansen
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Authors:  B J Bachmann
Journal:  Microbiol Rev       Date:  1990-06

5.  The Chlamydia trachomatis hyp operon is homologous to the groE stress response operon of Escherichia coli.

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6.  Trachoma agent DNA.

Authors:  I Sarov; Y Becker
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8.  Physical map of the Brucella melitensis 16 M chromosome.

Authors:  A Allardet-Servent; M J Carles-Nurit; G Bourg; S Michaux; M Ramuz
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9.  Chlamydia trachomatis RNA polymerase major sigma subunit. Sequence and structural comparison of conserved and unique regions with Escherichia coli sigma 70 and Bacillus subtilis sigma 43.

Authors:  J E Koehler; R R Burgess; N E Thompson; R S Stephens
Journal:  J Biol Chem       Date:  1990-08-05       Impact factor: 5.157

10.  Cloning and sequence of the gene for heat shock protein 60 from Chlamydia trachomatis and immunological reactivity of the protein.

Authors:  M C Cerrone; J J Ma; R S Stephens
Journal:  Infect Immun       Date:  1991-01       Impact factor: 3.441

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  14 in total

1.  T lymphocyte immunity in host defence against Chlamydia trachomatis and its implication for vaccine development.

Authors:  X Yang; R Brunham
Journal:  Can J Infect Dis       Date:  1998-03

2.  Physical and genetic map of the obligate intracellular bacterium Coxiella burnetii.

Authors:  H Willems; C Jäger; G Baljer
Journal:  J Bacteriol       Date:  1998-08       Impact factor: 3.490

3.  Characterization of a new isolate of Chlamydia trachomatis which lacks the common plasmid and has properties of biovar trachoma.

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Review 4.  Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing.

Authors:  F C Tenover; R D Arbeit; R V Goering; P A Mickelsen; B E Murray; D H Persing; B Swaminathan
Journal:  J Clin Microbiol       Date:  1995-09       Impact factor: 5.948

5.  Physical map of the genome of Planctomyces limnophilus, a representative of the phylogenetically distinct planctomycete lineage.

Authors:  N Ward-Rainey; F A Rainey; E M Wellington; E Stackebrandt
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6.  Genotypic identification and phylogenetic analysis of the spotted fever group rickettsiae by pulsed-field gel electrophoresis.

Authors:  V Roux; D Raoult
Journal:  J Bacteriol       Date:  1993-08       Impact factor: 3.490

7.  Genetic variability among Chlamydia trachomatis reference and clinical strains analyzed by pulsed-field gel electrophoresis.

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8.  Characterization of late gene promoters of Chlamydia trachomatis.

Authors:  M J Fahr; A L Douglas; W Xia; T P Hatch
Journal:  J Bacteriol       Date:  1995-08       Impact factor: 3.490

9.  Induction of interferon-stimulated genes by Chlamydia pneumoniae in fibroblasts is mediated by intracellular nucleotide-sensing receptors.

Authors:  Srikanth Chiliveru; Svend Birkelund; Søren R Paludan
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10.  Determination of genome sizes of Rickettsia spp. within the spotted fever group, using pulsed-field gel electrophoresis.

Authors:  V Roux; M Drancourt; D Raoult
Journal:  J Bacteriol       Date:  1992-11       Impact factor: 3.490

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