Douglas I Walker1, Kurt D Pennell, Karan Uppal, Xiaoyan Xia, Philip K Hopke, Mark J Utell, Richard P Phipps, Patricia J Sime, Patricia Rohrbeck, Col Timothy M Mallon, Dean P Jones. 1. Division of Pulmonary, Allergy and Critical Care Medicine, Department of Medicine, Emory University, Atlanta, Georgia (Mr Walker, Drs Uppal and Jones); Department of Civil and Environmental Engineering, Tufts University, Medford, Massachusetts (Mr Walker, Dr Pennell); Center for Air Resources Engineering and Science, Clarkson University, Potsdam (Drs Xia, Hopke); Pulmonary Division, Department of Medicine (Drs Utell, Phipps, Sime), Department of Environmental Medicine, University of Rochester Medical Center, New York (Drs Utell, Phipps, Sime); 779th Aerospace Medicine Squadron, 79th Medical Wing, Joint Base Andrews, MD (Dr Rohrbeck); and Professor, Department of Preventive Medicine and Biostatistics, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, MD 20814 (Dr Mallon).
Abstract
OBJECTIVE: A pilot study was conducted to test the feasibility of using Department of Defense Serum Repository (DoDSR) samples to study health and exposure-related effects. METHODS: Thirty unidentified human serum samples were obtained from the DoDSR and analyzed for normal serum metabolites with high-resolution mass spectrometry and serum levels of free benzo(a)pyrene (BaP) by gas chromatography-mass spectrometry. Metabolic associations with BaP were determined using a metabolome-wide association study (MWAS) and metabolic pathway enrichment. RESULTS: The serum analysis detected normal ranges of glucose, selected amino acids, fatty acids, and creatinine. Free BaP was detected in a broad concentration range. MWAS of BaP showed associations with lipids, fatty acids, and sulfur amino acid metabolic pathways. CONCLUSION: The results show that the DoDSR samples are of sufficient quality for chemical profiling of DoD personnel.
OBJECTIVE: A pilot study was conducted to test the feasibility of using Department of Defense Serum Repository (DoDSR) samples to study health and exposure-related effects. METHODS: Thirty unidentified human serum samples were obtained from the DoDSR and analyzed for normal serum metabolites with high-resolution mass spectrometry and serum levels of free benzo(a)pyrene (BaP) by gas chromatography-mass spectrometry. Metabolic associations with BaP were determined using a metabolome-wide association study (MWAS) and metabolic pathway enrichment. RESULTS: The serum analysis detected normal ranges of glucose, selected amino acids, fatty acids, and creatinine. Free BaP was detected in a broad concentration range. MWAS of BaP showed associations with lipids, fatty acids, and sulfur amino acid metabolic pathways. CONCLUSION: The results show that the DoDSR samples are of sufficient quality for chemical profiling of DoD personnel.
Authors: J D Pleil; M A Stiegel; J R Sobus; S Tabucchi; A J Ghio; M C Madden Journal: J Chromatogr B Analyt Technol Biomed Life Sci Date: 2010-04-29 Impact factor: 3.205
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Authors: Xiaoyan Xia; Alesia Carroll-Haddad; Nicole Brown; Mark J Utell; Col Timothy M Mallon; Philip K Hopke Journal: J Occup Environ Med Date: 2016-08 Impact factor: 2.162
Authors: Melissa P Osborn; Youngja Park; Megan B Parks; L Goodwin Burgess; Karan Uppal; Kichun Lee; Dean P Jones; Milam A Brantley Journal: PLoS One Date: 2013-08-27 Impact factor: 3.240
Authors: Yunfeng Huang; Qin Hui; Douglas I Walker; Karan Uppal; Jack Goldberg; Dean P Jones; Viola Vaccarino; Yan V Sun Journal: Epigenomics Date: 2018-03-12 Impact factor: 4.778
Authors: Young-Mi Go; Matthew R Smith; Douglas I Walker; Karan Uppal; Patricia Rohrbeck; Pamela L Krahl; Philip K Hopke; Mark J Utell; Timothy M Mallon; Dean P Jones Journal: J Occup Environ Med Date: 2019-12 Impact factor: 2.162
Authors: Thomas H Thatcher; Collynn F Woeller; Juilee Thakar; Atif Khan; Philip K Hopke; Matthew Ryan Smith; Karan Uppal; Douglas I Walker; Young-Mi Go; Dean P Jones; Pamela L Krahl; Timothy M Mallon; Patricia J Sime; Richard P Phipps; Mark J Utell Journal: J Occup Environ Med Date: 2019-12 Impact factor: 2.162
Authors: Juilee Thakar; Thomas H Thatcher; Matthew Ryan Smith; Collynn F Woeller; Douglas I Walker; Mark J Utell; Philip K Hopke; Timothy M Mallon; Pamela L Krahl; Patricia Rohrbeck; Young-Mi Go; Dean P Jones; Karan Uppal Journal: J Occup Environ Med Date: 2019-12 Impact factor: 2.162
Authors: Matthew Ryan Smith; Douglas I Walker; Karan Uppal; Mark J Utell; Philip K Hopke; Timothy M Mallon; Pamela L Krahl; Patricia Rohrbeck; Young-Mi Go; Dean P Jones Journal: J Occup Environ Med Date: 2019-12 Impact factor: 2.162
Authors: Douglas I Walker; M Elizabeth Marder; Yukiko Yano; Metrecia Terrell; Yongliang Liang; Dana Boyd Barr; Gary W Miller; Dean P Jones; Michele Marcus; Kurt D Pennell Journal: Environ Res Date: 2019-02-13 Impact factor: 6.498
Authors: Dean P Jones; Douglas I Walker; Karan Uppal; Patricia Rohrbeck; Col Timothy M Mallon; Young-Mi Go Journal: J Occup Environ Med Date: 2016-08 Impact factor: 2.162
Authors: Douglas I Walker; Col Timothy M Mallon; Philip K Hopke; Karan Uppal; Young-Mi Go; Patricia Rohrbeck; Kurt D Pennell; Dean P Jones Journal: J Occup Environ Med Date: 2016-08 Impact factor: 2.162