Literature DB >> 27498349

Bead-beating artefacts in the Bacteroidetes to Firmicutes ratio of the human stool metagenome.

Heidi C Vebø1, Magdalena Kauczynska Karlsson1, Ekaterina Avershina2, Lene Finnby1, Knut Rudi3.   

Abstract

We evaluated bead-beating cell-lysis in analysing the human stool metagenome, since this is a key step. We observed that two different bead-beating instruments from the same producer gave a three-fold difference in the Bacteroidetes to Firmicutes ratio. This illustrates that bead-beating can have a major impact on downstream metagenome analyses.
Copyright © 2016 Elsevier B.V. All rights reserved.

Entities:  

Keywords:  Bead-beating; Human; Metagenome

Mesh：

Substances：

Year:  2016        PMID： 27498349     DOI： 10.1016/j.mimet.2016.08.005

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN： 0167-7012            Impact factor:   2.363


  12 in total

1.  Comparison of Gut Microbiota Diversity Between Captive and Wild Tokay Gecko (Gekko gecko).

Authors:  Sanqi Tang; Yuhui Li; Chengming Huang; Shufa Yan; Yongtai Li; Zening Chen; Zhengjun Wu
Journal:  Front Microbiol       Date:  2022-06-17       Impact factor: 6.064

Review 2.  New opportunities for managing acute and chronic lung infections.

Authors:  William O C M Cookson; Michael J Cox; Miriam F Moffatt
Journal:  Nat Rev Microbiol       Date:  2017-10-24       Impact factor: 60.633

3.  Comparison of Meconium DNA Extraction Methods for Use in Microbiome Studies.

Authors:  Lisa F Stinson; Jeffrey A Keelan; Matthew S Payne
Journal:  Front Microbiol       Date:  2018-02-20       Impact factor: 5.640

Review 4.  Geography, Ethnicity or Subsistence-Specific Variations in Human Microbiome Composition and Diversity.

Authors:  Vinod K Gupta; Sandip Paul; Chitra Dutta
Journal:  Front Microbiol       Date:  2017-06-23       Impact factor: 5.640

Review 5.  Changes of Colonic Bacterial Composition in Parkinson's Disease and Other Neurodegenerative Diseases.

Authors:  Sara Gerhardt; M Hasan Mohajeri
Journal:  Nutrients       Date:  2018-06-01       Impact factor: 5.717

6.  Pre- and Post-Race Intestinal Microbiota in Long-Distance Sled Dogs and Associations with Performance.

Authors:  Kristoffer Relling Tysnes; Inga Leena Angell; Iselin Fjellanger; Sigrid Drageset Larsen; Silje Rebekka Søfteland; Lucy J Robertson; Ellen Skancke; Knut Rudi
Journal:  Animals (Basel)       Date:  2020-01-25       Impact factor: 2.752

7.  Culture dependent and independent analyses suggest a low level of sharing of endospore-forming species between mothers and their children.

Authors:  Ekaterina Avershina; Marte Gro Larsen; Marina Aspholm; Toril Lindback; Ola Storrø; Torbjørn Øien; Roar Johnsen; Knut Rudi
Journal:  Sci Rep       Date:  2020-02-04       Impact factor: 4.379

Review 8.  Methods for exploring the faecal microbiome of premature infants: a review.

Authors:  Jacob A F Westaway; Roger Huerlimann; Catherine M Miller; Yoga Kandasamy; Robert Norton; Donna Rudd
Journal:  Matern Health Neonatol Perinatol       Date:  2021-03-08

9.  Measuring and mitigating PCR bias in microbiota datasets.

Authors:  Justin D Silverman; Rachael J Bloom; Sharon Jiang; Heather K Durand; Eric Dallow; Sayan Mukherjee; Lawrence A David
Journal:  PLoS Comput Biol       Date:  2021-07-06       Impact factor: 4.475

10.  Gut microbiota of provisioned and wild rhesus macaques (Macaca mulatta) living in a limestone forest in southwest Guangxi, China.

Authors:  Ting Chen; Yuhui Li; Jipeng Liang; Youbang Li; Zhonghao Huang
Journal:  Microbiologyopen       Date:  2019-12-26       Impact factor: 3.139
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.