Judit Bassols1, Matteo Serino2,3, Gemma Carreras-Badosa1, Rémy Burcelin2,3, Vincent Blasco-Baque2,3,4, Abel Lopez-Bermejo1, José-Manuel Fernandez-Real5. 1. Department of Pediatrics, Dr. Josep Trueta Hospital and Girona Institute for Biomedical Research, Girona, Spain. 2. Institut National de la Santé et de la Recherche Médicale (INSERM), Toulouse, France. 3. Institut de Maladies Métaboliques et Cardiovasculaires (I2MC), Université Paul Sabatier (UPS), Toulouse, France. 4. Faculté de Chirurgie Dentaire de Toulouse, Université Paul Sabatier, Toulouse, France. 5. Department of Endocrinology, Dr. Josep Trueta Hospital and Girona Institute for Biomedical Research and CIBERobn, Girona, Spain.
Abstract
BACKGROUND: The human microbiota is a modulator of the immune system. Variations in the placental microbiota could be related with pregnancy disorders. We profiled the placental microbiota and microbiome in women with gestational diabetes (GDM) and studied its relation to maternal metabolism and placental expression of anti-inflammatory cytokines. METHODS: Placental microbiota and microbiome and expression of anti-inflammatory cytokines (IL10, TIMP3, ITGAX, and MRC1MR) were analyzed in placentas from women with GDM and from control women. Fasting insulin, glucose, O'Sullivan glucose, lipids, and blood cell counts were assessed at second and third trimester of pregnancy. RESULTS: Bacteria belonging to the Pseudomonadales order and Acinetobacter genus showed lower relative abundance in women with GDM compared to control (P < 0.05). In GDM, lower abundance of placental Acinetobacter associated with a more adverse metabolic (higher O'Sullivan glucose) and inflammatory phenotype (lower blood eosinophil count and lower placental expression of IL10 and TIMP3) (P < 0.05 to P = 0.001). Calcium signaling pathway was increased in GDM placental microbiome. CONCLUSION: A distinct microbiota profile and microbiome is present in GDM. Acinetobacter has been recently shown to induce IL-10 in mice. GDM could constitute a state of placental microbiota-driven altered immunologic tolerance, making placental microbiota a new target for therapy in GDM.
BACKGROUND: The human microbiota is a modulator of the immune system. Variations in the placental microbiota could be related with pregnancy disorders. We profiled the placental microbiota and microbiome in women with gestational diabetes (GDM) and studied its relation to maternal metabolism and placental expression of anti-inflammatory cytokines. METHODS: Placental microbiota and microbiome and expression of anti-inflammatory cytokines (IL10, TIMP3, ITGAX, and MRC1MR) were analyzed in placentas from women with GDM and from control women. Fasting insulin, glucose, O'Sullivan glucose, lipids, and blood cell counts were assessed at second and third trimester of pregnancy. RESULTS: Bacteria belonging to the Pseudomonadales order and Acinetobacter genus showed lower relative abundance in women with GDM compared to control (P < 0.05). In GDM, lower abundance of placental Acinetobacter associated with a more adverse metabolic (higher O'Sullivan glucose) and inflammatory phenotype (lower blood eosinophil count and lower placental expression of IL10 and TIMP3) (P < 0.05 to P = 0.001). Calcium signaling pathway was increased in GDM placental microbiome. CONCLUSION: A distinct microbiota profile and microbiome is present in GDM. Acinetobacter has been recently shown to induce IL-10 in mice. GDM could constitute a state of placental microbiota-driven altered immunologic tolerance, making placental microbiota a new target for therapy in GDM.
Authors: Nanna Fyhrquist; Lasse Ruokolainen; Alina Suomalainen; Sari Lehtimäki; Ville Veckman; Johanna Vendelin; Piia Karisola; Maili Lehto; Terhi Savinko; Hanna Jarva; Timo U Kosunen; Jukka Corander; Petri Auvinen; Lars Paulin; Leena von Hertzen; Tiina Laatikainen; Mika Mäkelä; Tari Haahtela; Dario Greco; Ilkka Hanski; Harri Alenius Journal: J Allergy Clin Immunol Date: 2014-09-26 Impact factor: 10.793
Authors: Kathleen M Antony; Jun Ma; Kristen B Mitchell; Diana A Racusin; James Versalovic; Kjersti Aagaard Journal: Am J Obstet Gynecol Date: 2014-12-31 Impact factor: 8.661
Authors: Lisa A Spencer; Craig T Szela; Sandra A C Perez; Casey L Kirchhoffer; Josiane S Neves; Amy L Radke; Peter F Weller Journal: J Leukoc Biol Date: 2008-10-07 Impact factor: 4.962
Authors: Kristen E Boyle; Hyonson Hwang; Rachel C Janssen; James M DeVente; Linda A Barbour; Teri L Hernandez; Lawrence J Mandarino; Martha Lappas; Jacob E Friedman Journal: PLoS One Date: 2014-09-12 Impact factor: 3.240
Authors: Amanda L Prince; Ryan M Pace; Tyler Dean; Diana Takahashi; Paul Kievit; Jacob E Friedman; Kjersti M Aagaard Journal: Am J Primatol Date: 2019-05-07 Impact factor: 2.371
Authors: Shreyas V Kumbhare; Dhrati V V Patangia; Ravindra H Patil; Yogesh S Shouche; Nitinkumar P Patil Journal: J Biosci Date: 2019-06 Impact factor: 1.826
Authors: Joe Jongpyo Lim; Moumita Dutta; Joseph L Dempsey; Hans-Joachim Lehmler; James MacDonald; Theo Bammler; Cheryl Walker; Terrance J Kavanagh; Haiwei Gu; Sridhar Mani; Julia Yue Cui Journal: Toxicol Sci Date: 2021-10-27 Impact factor: 4.109
Authors: Derrick M Chu; Gregory C Valentine; Maxim D Seferovic; Kjersti M Aagaard Journal: Gastroenterol Clin North Am Date: 2019-07-02 Impact factor: 3.806
Authors: Kevin R Theis; Roberto Romero; Andrew D Winters; Jonathan M Greenberg; Nardhy Gomez-Lopez; Ali Alhousseini; Janine Bieda; Eli Maymon; Percy Pacora; Jennifer M Fettweis; Gregory A Buck; Kimberly K Jefferson; Jerome F Strauss; Offer Erez; Sonia S Hassan Journal: Am J Obstet Gynecol Date: 2019-03 Impact factor: 10.693