| Literature DB >> 26154056 |
Lucile Garidou1, Céline Pomié2, Pascale Klopp2, Aurélie Waget2, Julie Charpentier2, Meryem Aloulou3, Anaïs Giry2, Matteo Serino2, Lotta Stenman4, Sampo Lahtinen4, Cedric Dray2, Jason S Iacovoni5, Michael Courtney6, Xavier Collet2, Jacques Amar7, Florence Servant6, Benjamin Lelouvier6, Philippe Valet2, Gérard Eberl8, Nicolas Fazilleau3, Victorine Douin-Echinard2, Christophe Heymes2, Rémy Burcelin9.
Abstract
A high-fat diet (HFD) induces metabolic disease and low-grade metabolic inflammation in response to changes in the intestinal microbiota through as-yet-unknown mechanisms. Here, we show that a HFD-derived ileum microbiota is responsible for a decrease in Th17 cells of the lamina propria in axenic colonized mice. The HFD also changed the expression profiles of intestinal antigen-presenting cells and their ability to generate Th17 cells in vitro. Consistent with these data, the metabolic phenotype was mimicked in RORγt-deficient mice, which lack IL17 and IL22 function, and in the adoptive transfer experiment of T cells from RORγt-deficient mice into Rag1-deficient mice. We conclude that the microbiota of the ileum regulates Th17 cell homeostasis in the small intestine and determines the outcome of metabolic disease.Entities:
Mesh：
Substances：
Year: 2015 PMID： 26154056 DOI： 10.1016/j.cmet.2015.06.001
Source DB: PubMed Journal: Cell Metab ISSN： 1550-4131 Impact factor: 27.287